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Imobilization of β-glucosidase using the cellulose-binding domain of Bacillus subtilis endo-β-1,4-glucanase

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Abstract

A recombinant plasmid pβCBD was constructed for immobilization of Cellulomonas fimi β-glucosidase (Cbg) using the cellulose-binding domain (CBD) of Bacillus subtilis BSE 616 endo-β-1,4-glucanase (Beg). The Cbg-CBD Beg fusion protein, 80 kDa, was expressed in Escherichia coli and immobilized to Avicel. Cellobiose was completely hydrolyzed with the immobilized fusion protein. The fusion protein bound to Avicel retained full activity during continuous operation for 24 h at 4°C.

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Authors and Affiliations

  1. R Center, Cheiljedang Cooperation, Ichon, Korea

    Dong Ho Ahn

  2. Department of Agricultural Chemistry, Sunchon National University, Sunchon, Korea

    Hoon Kim

  3. Department of Biotechnology, KAIST, Taejon, Korea

    Moo Young Pack

Authors
  1. Dong Ho Ahn
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  2. Hoon Kim
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  3. Moo Young Pack
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Ahn, D.H., Kim, H. & Young Pack, M. Imobilization of β-glucosidase using the cellulose-binding domain of Bacillus subtilis endo-β-1,4-glucanase. Biotechnology Letters 19, 483–486 (1997). https://doi.org/10.1023/A:1018360530691

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  • DOI: https://doi.org/10.1023/A:1018360530691

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