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Induced final maturation and ovulation in a small anabantoid teleost, the dwarf gourami, Colisa lalia. I. The effects of gonadotrophic preparations, LHRHa, steroids and thyroid hormones

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Aquarium Sciences and Conservation

Abstract

Colisa lalia (Ham.) has been used as a model for the development of techniques for induced spawning that are applicable to small teleosts where ovulation requires prolonged exposure to suitable breeding conditions.

Human chorionic gonadotrophin (hCG; ≥ 4 IU per fish) induced ovulation within 24 hours, whereas homologous pituitary extracts were relatively ineffective. When administered in saline (≤ 20 μg per fish per injection), des-Gly10,[D-Ala6]- LHRH N-ethylamide (LHRHa) was ineffective, but it stimulated ovulation in a proportion of fish when administered (≥ 1.6 μg per fish) as an emulsion in Freund's incomplete adjuvant (FIA). Together, these results suggest that ovulation requires the synthesis as well as the secretion of gonadotrophin in C. lalia.

Long-term treatment with thyroid hormones appeared to enhance the ovulatory response to LHRHa in FIA, possibly by effects on the ovary; whereas the various steroids tested were ineffective at the dosages used

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  1. Fish Neurobiology Lab, School of Biological Sciences, The National University, Lower Kent Ridge Rd, Singapore, Singapore

    N.K. Ng , D. Tsi  & A.D. Munro

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Ng , N., Tsi , D. & Munro , A. Induced final maturation and ovulation in a small anabantoid teleost, the dwarf gourami, Colisa lalia. I. The effects of gonadotrophic preparations, LHRHa, steroids and thyroid hormones. Aquarium Sciences and Conservation 1, 169–188 (1997). https://doi.org/10.1023/A:1018352121469

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