Abstract
The use of recombinant gonadotropin-releasing hormone (rGnRH) has very rarely been tested in fish to promote spawning. This study evaluated the impact of recombinant chicken gonadotropin-releasing hormone (rcGnRH) with metoclopramide on the release of sex steroids and final maturation induction in goldfish (Carassius auratus) broodstock. For this purpose, goldfish broodstock was divided into four groups and treated with 0.9% NaCl with 20 mg/kg metoclopramide (Met) (C); 10 μg/kg body weight (BW) rcGnRH with 20 mg/kg metoclopramide (rcGn10); 15 μg/kg BW rcGnRH with 20 mg/kg metoclopramide (rcGn15); and 20 μg/kg BW rcGnRH with 20 mg/kg metoclopramide (rcGn20). The capability of the rcGnRH for eliciting biological response was tested in vivo by evaluating the changes of 17β estradiol (E2), testosterone (T), and 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) and the induced spawning. Blood samples were obtained at 0 h, 12 h, and 24 h after injection. The rcGn10, rcGn15, and rcGn20 treatments induced lower E2 concentration, especially 24 h post-injection. T levels were significantly higher in rcGn10, rcGn15, and rcGn20 treatments 12 h post-injection than at 0 h and then decreased at 24 h post-injection. Furthermore, the rcGnRH tested significantly enhanced DHP secretion in rcGn10, rcGn15, and rcGn20 treatments 12 h post-injection before a decline at 24 h post-injection. No significant difference between the sampling times was found in the C treatment for the 3 sex steroids tested. The results also displayed that rcGnRH at 10–20 µg/kg of body weight can trigger spawning with the highest speed and efficiency of spawning at 20 µg/kg. The obtained results represent a possible strategy for enhancing the artificial reproduction and ovulation of broodstock fish by rGnRH and further support the use of recombinant hormones to promote reproduction in aquaculture.
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Data availability
The data and materials that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors thank the Fisheries Labs, Sari Agricultural Sciences, and Natural Resources University for its support.
Funding
The Iran Biotechnology Development Council under project No. 970202 funded this work. Moreover, this research was supported by University of Zabol (Iran) with grant code (UOZ-GR.9718:51).
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Sedigheh Mohammadzadeh: conceptualization, methodology, software, data curation, and writing- original draft preparation; Sylvain Milla: reviewing and editing; Ehsan Ahmadifar: reviewing and editing; Masoumeh Karimi: software and editing; Mahmoud A.O. Dawood: reviewing and editing.
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Mohammadzadeh, S., Milla, S., Ahmadifar, E. et al. Is the use of recombinant cGnRH may be a future alternative to control the fish spawning? Let us go with the goldfish example. Fish Physiol Biochem 47, 951–960 (2021). https://doi.org/10.1007/s10695-021-00953-6
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DOI: https://doi.org/10.1007/s10695-021-00953-6