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Chromosomal regions controlling anther culturability in rice (Oryza sativa L.)

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Abstract

Diallel analysis has revealed that anther culturability in rice (Oryza sativa L.) is a quantitative trait controlled by the nuclear genome. Mapping of anther culturability is important to increase the efficiency for green plant regeneration from microspores. In the previous study, we detected distorted segregation of RFLP markers in rice populations derived from the anther culture of an F1 hybrid between a japonica cultivar ‘Nipponbare’ and an indica cultivar ‘Milyang 23’. To clarify the association between chromosomal regions showing distorted segregation and anther culturability, the anther culturability of doubled haploid lines derived from the same cross combination was examined, and the association between alleles of the RFLP markers, which exhibiting the most distorted segregation on chromosomes 1, 3, 7, 10 and 11, and the anther culturability was evaluated. One region on chromosome 1 was found to control callus formation from microspores, and one region on chromosome 10 appeared to control the ratio of green to albino regenerated plants. In both regions, the Nipponbare allele had positive effects. Three regions on chromosomes 3, 7 and 11, however, showed no significant effect on anther culturability.

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Author notes

  1. Masumi Yamagishi

    Present address: Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, 690-0823, J, apan

Authors and Affiliations

  1. Nonoichi-machi, Research Institute of Agricultural Resources, Ishikawa Agricultural College, Ishikawa, 921, Japan

    Masumi Yamagishi, Motoyasu Otani & Mariko Higashi

  2. Kaga Agricultural Extension Center, Saiwai, Kaga, Ishikawa, 922, Japan

    Mariko Higashi

  3. Hokuriku National Agricultural Experiment Station, MAFF, Inada, Joetsu, Niigata, 943-01, Japan

    Yoshimichi Fukuta & Kiichi Fukui

  4. Rice Genome Research Program, National Institute of Agrobiological Resources, MAFF, Kannondai, Tsukuba, Ibaraki, 305, Japan

    Takiko Shimada

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  1. Masumi Yamagishi
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  4. Yoshimichi Fukuta
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Yamagishi, M., Otani, M., Higashi, M. et al. Chromosomal regions controlling anther culturability in rice (Oryza sativa L.). Euphytica 103, 227–234 (1998). https://doi.org/10.1023/A:1018328708322

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