Skip to main content
SpringerLink
Log in

Efficient in vitro shoot regeneration responses of Phaseolus vulgaris and P. coccineus

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Phaseolus vulgaris L. is the most important economic species within the genus Phaseolus, and it is grown in all parts of the world. Genetic improvement by conventional breeding has met considerable success, although production of hybrids between species within the genus has been limited due to sexual incompatibility or other evolutionary lethalities. Recent advances in tissue culture have offered the opportunity to produce cultivars which could not be obtained by conventional breeding methods, but regeneration protocols are influenced by the genotype. A standard regeneration procedure was assessed for its applicability to elite breeding lines of P. vulgaris L. and landraces of P. coccineus L. from seedling explants containing a cotyledon and a small portion of the split embryonic axis. In vitro culture response and regeneration ability varied significantly between species and amongst genotypes. P. coccineus produced more shoots per explant with a higher rooting efficiency than P. vulgaris. These significant genotype effects suggest that genetic factors are important in the response to in vitro tissue culture. Different genotypes were identified, which were more competent to in vitro culture and could produce highly responsible hybrids. This in vitro culture system carry a high potential for propagation of P. vulgaris and P. coccineus, and subsequent exploitation of hybrid forms, which could also be incorporated into somatic cell approaches to improve these species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abawi, G.S., R. Providenti, D.C. Crosier & J.E. Hunter, 1978. Inheritance of resistance to white mold disease in Phaseolus coccineus. J Her 69: 200–205.

    Google Scholar 

  • Anderson, N.O., P.D. Ascher & K. Haghighi, 1996. Congruity back-crossing as a means of creating genetic variability in self pollinated crops: seed morphology of Phaseolus vulgaris L. and P. acutifolius A. Gray hybrids. Euphytica 87: 211–224.

    Article  Google Scholar 

  • Angelini, R.R. & A. Allavena, 1989. Plant regeneration from immature cotyledon explant cultures of bean (P. coccineus L.). Plant Cell, Tiss Org Cult 19: 167–174.

    Article  Google Scholar 

  • Becraft, P.W. & G.A. Taylor, 1992. Genetic variation for anther culture inducibility in crosses of highly culturable winter wheats. Plant Breed 108: 19–25.

    Article  Google Scholar 

  • Birhman, R.K., G. Laublin & M. Cappadocia, 1994. Genetic control of in vitro shoot regeneration from leaf explants in Solanum chacoense Bitt. Theor Appl Genet 88: 535–540.

    Article  Google Scholar 

  • Coyne, D.P., M.L. Schuster & S. Al-Yasiri, 1963. Reaction studies of bean species and varieties to common blight and bacterial wilt. Plant Dis Rep 47: 534–537.

    Google Scholar 

  • De Buyser, J., J.L. Marcotte & Y. Henry, 1992. Genetic analysis of in vitro wheat somatic embryogenesis. Euphytica 63: 265–270.

    Google Scholar 

  • Fernandez-Caso, M., M.I. Pelaez & M.L. Ruiz, 1996. Onset of in vitro morphogenetic responses and protein pattern changes in Phaseolus vulgaris L. J Plant Physiol 149: 757–761.

    CAS  Google Scholar 

  • Franklin, C.I., T.N. Trieu, R.A. Gonzalez & R.A. Dixon, 1991. Plant regeneration from seedling explants of green bean (Phaseolus vulgaris L.) via organogenesis. Plant Cell, Tiss Org Cult 24: 199–206.

    Article  Google Scholar 

  • Freytag, G.F., M.J. Basset & M. Zapata, 1982. Registration of XR, 235-1-1 bean germplasm. Crop Sci 22: 1268.

    Article  Google Scholar 

  • Gomez, K.A. & A.A. Gomez, 1984. Statistical procedures for agricultural research. John Wiley and Sons (2nd ed.), New York.

    Google Scholar 

  • Homma, S. & O. Heecket, 1958. Bean interspecific hybrid involving P. coccineus and P. lunatus. Proc Amer Soc Hort Sci 72: 360–365.

    Google Scholar 

  • Henry, Y., P. Vain & De Buyser, 1994. Genetic analysis of in vitro plant tissue culture responses and regeneration capacities. Euphytica 79: 45–48.

    Article  Google Scholar 

  • Hunter, J.E., M.H. Dickson & M.A. Boettger, 1982. Evaluation of plant introduction of Phaseolus spp. for resistance to white mold. Plant Dis 66: 320–324.

    Article  Google Scholar 

  • Keyes, G.J., G.B. Collins & N.L. Taylor, 1980. Genetic variation in tissue cultures of red clover. Theor Appl Genet 58: 266–271.

    Article  Google Scholar 

  • Kim, J.W. & T. Minamikawa, 1997. Stable delivery of a canavalin promoter-β-glucoronidase gene fusion into French bean by particle bombardment. Plant Cell Physiol 38(1): 70–75.

    CAS  Google Scholar 

  • Malik, K.A. & R.K. Saxena, 1991. Regeneration in Phaseolus vulgaris. Planta 184: 148–150.

    Article  CAS  Google Scholar 

  • Malik, K.A. & R.K. Saxena, 1992. Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius A., P. aureus (L.) Wilczek, P. coccineus L., and P. wrightii L. Plant Cell Rep 11: 163–168.

    Article  CAS  Google Scholar 

  • Masson, J. & J.P. Paskowski, 1992. The culture response of Arabidopsis thaliana protoplasts is determined by the growth conditions of donor plants. The Plant J 2: 829–833.

    Google Scholar 

  • McClean, P. & K.F. Grafton, 1989, Regeneration of dry bean (Phaseolus vulgaris L.) via organogenesis. Plant Sci 60: 177–122.

    Article  Google Scholar 

  • Miah, M.A.A., E.D. Earle & G.S. Kush, 1985. Inheritance of callus formation ability in anther cultures of rice, Oryza sativa L. Theor Appl Genet 70: 113–116.

    Google Scholar 

  • Mohamed, M.F., P.E. Read & D.P. Coyne, 1991. Plant regeneration in vitro from the embryonic axes of common and tepary beans. Annu Rep Bean Improv Coop (Bic) 34: 150–151.

    Google Scholar 

  • Mohamed, F.M., P.E. Read & D.P. Coyne, 1992. Dark preconditioning, CPPU, and Thidiazuron promote shoot organogenesis on seedling node explants of common and faba beans. J Amer Soc Hort Sci 177(4): 668–672.

    Google Scholar 

  • Mohamed, F.M., D.P. Coyne & P.E. Read, 1993. Shoot organogenesis in callus induced from pedicel explants of common bean (Phaseolus vulgaris L.). J Amer Soc Hort Sci 188 10: 158–162.

    Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.

    Article  CAS  Google Scholar 

  • Nagl, W., S. Ignacimuthu & J. Becker, 1997. Genetic engineering and regeneration of Phaseolus and Vigna. State of the art and new attempts. J Plant Physiol 150: 625–644.

    CAS  Google Scholar 

  • Rabakoarihanta, A. M.C. Mok & D.W.S. Mok, 1982. Embryo development in reciprocal crosses of Phaseolus vulgaris L. and P. coccineus Lam. Theor Appl Genet 62: 59–64.

    Google Scholar 

  • Ramsay, G., 1993. Regeneration in grain legume tissue culture. Grain Legumes (Paris) 2: 16–17.

    Google Scholar 

  • SAS, Institute, 1982. SAS user's guide, Statistics, SAS Institute, Cary, NC.

    Google Scholar 

  • Schmidt, E.D.L., A.J. De Jong & S.C. De Vries, 1994. Signal molecules involved in plant embryogenesis. Plant Molec Biol 26: 1305–1313.

    Article  CAS  Google Scholar 

  • Smartt, J., 1970. Interspecific hybridization between cultivated American species of the genus Phaseolus. Euphytica 19: 480–489.

    Article  Google Scholar 

  • Smartt, J., 1981. Gene pools in Phaseolus and Vigna cultigens. Euphytica 30: 445–449.

    Article  Google Scholar 

  • Singh, S.P., 1992. Common bean improvement in the tropics. Plant Breed Rev 10: 199–269.

    Google Scholar 

  • Taylor, T.E. & R.E. Veilleux, 1992. Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and correlations among them. Plant Cell, Tiss Org Cult 31: 95–103.

    Article  Google Scholar 

  • Thomas, H., 1964. Investigations into the inter-relationships of Phaseolus vulgaris L. and P. coccineus Lam. Genetica 35: 59–74.

    Article  Google Scholar 

  • Vaquero, F., C. Robles & M.L. Ruiz, 1993. A method for long-term micropropagation of Phaseolus coccineus. Plant Cell Rep 12: 395–398.

    Article  CAS  Google Scholar 

  • Wan, Y., T.R. Rocheford & J.M. Widholm, 1992. RFLP analysis to identify putative chromosomal regions involved in the anther culture response and callus formation of maize. Theor Appl Genet 86: 360–365.

    Google Scholar 

  • Yerkes, W.D. & G.F. Freytag, 1956. Phaseolus coccineus as a source of root resistance for the common bean. Phytopathology 46: 32–36.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Breeding Department, Mision Biologica de Galicia, CSIC, 36080, Pontevedra, Spain

    Marta Santalla

  2. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, Nottingham, NG7 2RD, U.K

    J. Brian Power & Michael R. Davey

Authors
  1. Marta Santalla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Brian Power
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael R. Davey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Santalla, M., Power, J.B. & Davey, M.R. Efficient in vitro shoot regeneration responses of Phaseolus vulgaris and P. coccineus. Euphytica 102, 195–202 (1998). https://doi.org/10.1023/A:1018317327302

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018317327302

Search

Navigation