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Production of alkaline protease by an alkaliphilic bacteria isolated from an alkaline soda lake

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Abstract

A new alkaliphilic strain of Microbacterium producing an alkaline protease was isolated from an alkaline soda lake in Ethiopia. High level of protease activity was produced in the presence of glucose and sucrose as carbon sources. The optimum temperature and pH for activity were 65°C and 9.5-11.5 respectively. Above 50°C, Ca2+ was required for enzyme activity and stability. At 55 and 60°C it retained 100 and 85% of its original activity respectively after 1 h incubation. The enzyme was stable over the pH range of 5-12.

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Author notes

  1. Berhanu A. Gashe

    Present address: Department of Biological Sciences, University of Botswana, Private Bag 0022, Gaborone, Botswana

Authors and Affiliations

  1. Department of Biology, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia

    Amare Gessesse

Authors
  1. Amare Gessesse
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  2. Berhanu A. Gashe
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Gessesse, A., Gashe, B.A. Production of alkaline protease by an alkaliphilic bacteria isolated from an alkaline soda lake. Biotechnology Letters 19, 479–481 (1997). https://doi.org/10.1023/A:1018308513853

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