Skip to main content
SpringerLink
Log in

Genetic analysis of acetan biosynthesis in Acetobacter xylinum: DNA sequence analysis of the aceM gene encoding an UDP-glucose dehydrogenase

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Analysis of the nucleotide sequence of a 1592 bp region of Acetobacter xylinum genomic DNA involved in acetan biosynthesis revealed the presence of an open-reading frame (aceM) encoding a protein of 449 amino acids with a molecular weight of 48.5 kDa. The deduced amino acid sequence of aceM displayed high homology to the protein sequences of genes encoding UDP-glucose dehydrogenase (UGDH) activities from other organisms. AceM is likely to encode the UGDH involved in the biosynthesis of UDP-glucuronic acid required for acetanbiosynthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altschul S.F., Gish W., Miller W., Myers E.W. and Lipman D.J. (1990). J. Mol. Biol. 215, 403–410.

    Article  PubMed  CAS  Google Scholar 

  • Arrecubieta C., Garcia E. and Lopez R. (1995). Gene 167, 1–7.

    Article  PubMed  CAS  Google Scholar 

  • Arrecubieta C., Lopez R. and Garcia E. (1994). J. Bactiol. 176, 6375–6383.

    CAS  Google Scholar 

  • Bastin D.A., Stevenson G., Brown P.K., Haase A. and Reeves P.R. (1993). Mol. Micro. 7, 725–734.

    Article  CAS  Google Scholar 

  • Brown R.M. Jr. (1989). Bacterial Cellulose. In: Cellulose: Structural and Functional Aspects, I.F. Kennedy, G.O. Phillips and P.A. Williams, eds. pp. 145–151, Chichester: Ellis Horwood Ltd.

    Google Scholar 

  • Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D. et al. (1996). Sci. 273, 1058–1073.

    Article  CAS  Google Scholar 

  • Campos M.-E., Martinez-Salazar J.M., Lloret L., Moreno S., Nunez C., Espin G. and Soberon-Chavez G. (1996). J. Bactiol. 178, 1793–1799.

    CAS  Google Scholar 

  • Colquhoun I.J., Defernez M. and Morris V.J. (1995). Carb. Res. 269, 319–331.

    Article  CAS  Google Scholar 

  • Comstock L.E., Michalski J.M., Johnson J.A., Morris J.G. Jr. and Kaper J.B. (1996). Mol. Micro. 19, 815–826.

    Article  CAS  Google Scholar 

  • de Iannino N.I., Couso R.O. and Dankert M.A. (1988). J. Gen. Micro. 134, 1731–1736.

    CAS  Google Scholar 

  • Devereux J., Haeberli P. and Smithies O. (1984). N.A.R. 12, 216–223.

    Google Scholar 

  • Dougherty B.A. and van de Rijn I. (1993). J. Biol. Chem. 268, 7118–7124.

    PubMed  CAS  Google Scholar 

  • Gasser C.S., Winters J.A., Hironaka C.M. and Shah D.M. (1988). J. Biol. Chem. 263, 4280–4289.

    PubMed  CAS  Google Scholar 

  • Gold L. and Stormo G. (1987). Translational Initiation. In: Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, vol 2, F.C. Neidhardt, J.L. Ingraham, K.B. Low, B. Magasanik, M. Schaechter and H.E. Umbarger, eds. pp. 1302–1307, Washington, D.C.: American Society for Microbiology.

    Google Scholar 

  • Griffin A.M., Morris V.J. and Gasson M.J. (1994). Int. J. Biol. Macromol. 116, 287–289.

    Article  Google Scholar 

  • Griffin A.M., Morris V.J. and Gasson M.J. (1996a). FEMS Microb. Letts. 137, 115–121.

    Article  CAS  Google Scholar 

  • Griffin A.M., Morris V.J. and Gasson M.J. (1996b). DNA Seq.: J. DNA. Seq. Map. 6, 275–284.

    CAS  Google Scholar 

  • Harley C.B. and Reynolds P.R. (1987). N.A.R. 15, 2343–2361.

    CAS  Google Scholar 

  • Hempel J., Perozich J., Romovacek H., Hinich A., Kuo I. and Feingold D.S. (1994). Protein Sci. 3, 1074–1080.

    Article  PubMed  CAS  Google Scholar 

  • Higgins D.G. (1994). CLUSTAL V: Multiple Alignment of DNA and Protein Sequences. In: Computer Analysis of Sequence Data. Part 2, A.M. Griffin and H.G. Griffin, eds. pp. 307–318, Totowa, New Jersey: Humana Press.

    Chapter  Google Scholar 

  • Huang J. and Schell M. (1995). Mol. Micro. 16, 977–989.

    Article  CAS  Google Scholar 

  • Jelesko J.G. and Leigh J.A. (1994). Mol. Micro. 11, 165–173.

    Article  CAS  Google Scholar 

  • Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kirura T., Hosouchi T. and et al., (1996). DNA Res. 3, 109–136.

    Article  PubMed  CAS  Google Scholar 

  • Kiino D.R., Licudine R., Wilt K., Yang D.H.C. and Rothman-Denes L.B. (1993). J. Bactiol. 175, 7074–7080.

    CAS  Google Scholar 

  • Lin C.S., Lin N.T., Yang B.Y., Weng S.F. and Tseng Y.H. (1995). Biochem. Biophys. Res. Commun. 207, 223–230.

    Article  PubMed  CAS  Google Scholar 

  • Margulis L. (1970). Eucaryotic plants. In: Origin of Eucaryotic Cells, L. Margulis, ed. pp. 276–293, New Haven, CT: Yale University Press.

    Google Scholar 

  • Miller J.H. (1972). Experiments in Molecular Genetics, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Morona R., Van den Bosh L. and Manning P.A. (1995). J. Bactiol. 177, 1059–1068.

    CAS  Google Scholar 

  • Pearson W.R. (1994). Using the FASTA program to search protein and DNA sequence databases. In: Computer Analysis of Sequence Data. Part 1, A.M. Griffin and H.G. Griffin, eds. pp. 307–331, Totowa, New Jersey: Humana Press.

    Chapter  Google Scholar 

  • Petit C., Rigg G.P., Pazzani C., Smith A., Sieberth V., Stevens M., Boulnois G., Jann K. and Roberts I.S. (1995). Mol. Micro. 17, 611–620.

    Article  CAS  Google Scholar 

  • Roller S. and Dea I.C.M. (1992). Critical Rev. Biotech. 12, 261–277.

    CAS  Google Scholar 

  • Roychoudhury S., May T.B., Gill J.F., Singh S.K., Feingold D.S. and Chakrabarty A.M. (1989). J. Biol. Chem. 264, 9380–9385.

    PubMed  CAS  Google Scholar 

  • Sambrook J., Fritsch E.F. and Maniatis T. (1989). Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Scrutton N.S., Berry A. and Perham R.N. (1990). Nature 343, 38–43.

    Article  PubMed  CAS  Google Scholar 

  • Sievers M., Ludwig W. and Teuber M. (1994). System. Appl. Microbiol. 17, 189–196.

    CAS  Google Scholar 

  • Standal R., Iversen T.G., Coucheron D.H., Fjaervik E., Blatny J.M. and Valla S. (1994). J. Bactiol. 176, 665–672.

    CAS  Google Scholar 

  • Sutherland I.W. (1990). Biotechnology of Microbial Exopolysaccharides, Cambridge University Press.

  • Weeden N.F. (1981). J. Mol. Evol. 17, 133–139.

    Article  PubMed  CAS  Google Scholar 

  • Wierenga R.K., Terpstra P. and Hol W.G.J. (1986). J. Mol. Biol. 187, 101–107.

    Article  PubMed  CAS  Google Scholar 

  • Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., et al. (1994). Nature. 368, 32–38.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Norwich Laboratory, Institute of Food Research, Norwich Research Park, Colney Norwich, NR4 7UA, UK

    Annette M. Griffin, Kirstin J. Edwards, Victor J. Morris & Michael J. Gasson

Authors
  1. Annette M. Griffin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kirstin J. Edwards
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Victor J. Morris
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael J. Gasson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Griffin, A.M., Edwards, K.J., Morris, V.J. et al. Genetic analysis of acetan biosynthesis in Acetobacter xylinum: DNA sequence analysis of the aceM gene encoding an UDP-glucose dehydrogenase. Biotechnology Letters 19, 469–474 (1997). https://doi.org/10.1023/A:1018304412944

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018304412944

Keywords

Search

Navigation