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Protein concentration effect on protein-lipopolysaccharide (LPS) binding and endotoxin removal

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Abstract

It is known some proteins can disaggregate endotoxins and form complexes with lipopolysaccharide (LPS). Nevertheless, how protein concentration affects protein-LPS binding and endotoxin removal is unknown. In this study, protein samples at various concentrations were incubated with endotoxin samples at a fixed concentration. The mixtures were filtered by ultrafiltration membranes. As protein concentration increased, the amount of endotoxin detected in the filtrates increased too. This result indicates protein concentration has significant effect on protein-LPS binding and the amount of endotoxin disaggregated.

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Authors and Affiliations

  1. Department of Chemical Engineering, Chemistry and Environmental Science, New Jersey Institute of Technology, University Heights, Newark, New Jersey, 07102, USA

    Liping Li & Robert G. Luo

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  1. Liping Li
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  2. Robert G. Luo
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Li, L., Luo, R.G. Protein concentration effect on protein-lipopolysaccharide (LPS) binding and endotoxin removal. Biotechnology Letters 19, 135–138 (1997). https://doi.org/10.1023/A:1018304128932

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  • DOI: https://doi.org/10.1023/A:1018304128932

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