Abstract
Illumination of the dark-incubated cells of Synechocystis PCC6803 caused recovery of both respiratory activity of oxygen uptake and PS I-cyclic electron flow, which was monitored by the dark reduction of P700+ in the presence of DCMU after a 50 ms pulse light (MT) under background far-red light, but the effects were much smaller in those of the mutant M55, which has an ndh-B defective gene. Activity of an NADPH-NBT oxidoreductase with a higher molecular mass (around 380 kDa), which was only found in wild type but not in M55, became evident after the dark-incubated cells were exposed to the light. Immuno-blotting analysis indicated that the NADPH-NBT oxidoreductase contains the NdhB subunit of NDH. The expression of NdhB decreased in dark-incubated cells and increased upon transfer of the cells back to light. These results indicate that an NADPH-specific NDH participates in the light-regulated cyclic electron transport around Photosystem I as well as in respiratory electron transport to the intersystem chain in Synechocystis 6803.
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Mi, H., Deng, Y., Tanaka, Y. et al. Photo-induction of an NADPH dehydrogenase which functions as a mediator of electron transport to the intersystem chain in the cyanobacterium Synechocystis PCC6803. Photosynthesis Research 70, 167–173 (2001). https://doi.org/10.1023/A:1017946524199
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DOI: https://doi.org/10.1023/A:1017946524199