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Respiratory terminal oxidases alleviate photo-oxidative damage in photosystem I during repetitive short-pulse illumination in Synechocystis sp. PCC 6803

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Abstract

Oxygenic phototrophs are vulnerable to damage by reactive oxygen species (ROS) that are produced in photosystem I (PSI) by excess photon energy over the demand of photosynthetic CO2 assimilation. In plant leaves, repetitive short-pulse (rSP) illumination produces ROS to inactivate PSI. The production of ROS is alleviated by oxidation of the reaction center chlorophyll in PSI, P700, during the illumination with the short-pulse light, which is supported by flavodiiron protein (FLV). In this study, we found that in the cyanobacterium Synechocystis sp. PCC 6803 P700 was oxidized and PSI was not inactivated during rSP illumination even in the absence of FLV. Conversely, the mutant deficient in respiratory terminal oxidases was impaired in P700 oxidation during the illumination with the short-pulse light to suffer from photo-oxidative damage in PSI. Interestingly, the other cyanobacterium Synechococcus sp. PCC 7002 could not oxidize P700 without FLV during rSP illumination. These data indicate that respiratory terminal oxidases are critical to protect PSI from ROS damage during rSP illumination in Synechocystis sp. PCC 6803 but not Synechococcus sp. PCC 7002.

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Acknowledgements

The authors thank Prof. Akihiko Kondo, Prof. Tomohisa Hasunuma, and Dr. Shimpei Aikawa (Kobe University) for supplying the Synechococcus sp. PCC 7002 wild type. This work was supported by the Japan Society for the Promotion of Science (JSPS; Grant No. 26450079 to C.M.) and by the Core Research for Evolutional Science and Technology (CREST) division of the Japan Science and Technology Agency (Grant No. AL65D21010 to C.M.). G.S. is supported by a JSPS research fellowship (Grant No. 16J03443). We would like to thank Editage (http://www.editage.jp) for English language editing.

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  1. Department of Biological and Environmental Science, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan

    Ginga Shimakawa & Chikahiro Miyake

  2. Core Research for Environmental Science and Technology, Japan Science and Technology Agency, 7 Goban-cho, Chiyoda-ku, Tokyo, 102-0076, Japan

    Chikahiro Miyake

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  1. Ginga Shimakawa
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  2. Chikahiro Miyake
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CM conceived the original screening and research plans; CM supervised the experiments; GS performed all of the experiments; CM and GS designed the experiments and analyzed the data; CM and GS conceived the project and wrote the article.

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Correspondence to Chikahiro Miyake.

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Shimakawa, G., Miyake, C. Respiratory terminal oxidases alleviate photo-oxidative damage in photosystem I during repetitive short-pulse illumination in Synechocystis sp. PCC 6803. Photosynth Res 137, 241–250 (2018). https://doi.org/10.1007/s11120-018-0495-y

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