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Effect of Some Antibiotics on the In Vitro Morphogenetic Response from Callus Cultures of Coryphantha Elephantidens

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Biologia Plantarum

Abstract

The effect of five antibiotics: carbenicillin, chloramphenicol, cefotaxime, kanamycin and hygromycin on the organogenesis from callus cultures of Coryphantha elphantidens (Lem.) Lem. have been studied. Carbenicillin and cefotaxime stimulated shoot regeneration from callus. All antibiotics under study suppressed rooting of in vitro formed shoots. After five sequential subcultures on kanamycin supplemented medium, antibiotic resistant callus was obtained. To study the impact of kanamycin on resistant callus, total protein content was also studied. Selected callus showed a remarkable increase in callus mass. Antibiotic resistant plants have been selected by screening callus pieces on kanamycin supplemented media. Total protein content increased with subsequent subcultures in kanamycin resistant callus. The kanamycin selected shoots withstood the stability test after 2 months on antibiotic free medium. Plants were raised from the callus, which formed roots in 20 mg dm−3 kanamycin, which was under study.

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References

  • Barrett, C., Cassells, A.C.: An evaluation of antibiotics for the elimination of Xanthomonas campestris pv. pelargonii (Brown) from Pelargonium × domesticum cv. "Grand Slam".-Plant Cell Tissue Organ Cult. 36: 169–175, 1994.

    Google Scholar 

  • Berdy, J.: Handbook of Antibiotics.-CRC Press, Boca Raton 1982.

    Google Scholar 

  • Bhau, B.S.: Micropropagation of cacti through tissue culture.-J. indian Soc. Cactus Succulents 1: 12–14, 1999.

    Google Scholar 

  • Borrelli, G.M., DiFonzo, N., Lupotto, E.: Effect of cefotaxime on callus culture and plant regeneration in durum wheat.-J. Plant Physiol. 140: 372–374, 1992.

    Google Scholar 

  • Chang, C.C., Schmidt, D.R.: Initiation and proliferation of carrot callus using a combination of antibiotics.-Planta 185: 523–526, 1991.

    Google Scholar 

  • Cornu, D., Michel, M.F.: Bacteria contaminants in shoot cultures of Prunus avium L.: choice and phytotoxicity of antibiotics.-Acta Hort. 212: 83–86, 1987.

    Google Scholar 

  • Dodds, J.H., Roberts, L.W.: Some inhibitory effects of gentamycin on plant tissue cultures.-In Vitro cell. develop. Biol. 17: 467–470, 1981.

    Google Scholar 

  • Duncan, D.B.: Multiple range and multiple F-test.-Biometrics 11: 1–42, 1955.

    Google Scholar 

  • Eapen, S., George, L.: Influence of phytohormones, carbohydrates, amino acids, growth supplements and antibiotics on somatic embryogenesis and plant differentiation in finger millet.-Plant Cell Tissue Organ Cult. 22: 87–93, 1990.

    Google Scholar 

  • Falkiner, F.R.: Strategy for the selection of antibiotics for use against common bacterial pathogens and endophytes of plants.-Acta Hort. 225: 53–57, 1988.

    Google Scholar 

  • Falkiner, F.R.: The criteria for choosing an antibiotic for control of bacteria in plant tissue culture.-Int. Assoc. Plant Tissue Cult. Newslett. 60: 13–23, 1990.

    Google Scholar 

  • Fiola, J.A., Hassan, M.A., Swartz, H.J., Bors, R.H., McNicols, R.: Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves.-Plant Cell Tissue Organ Cult. 20: 223–228, 1990.

    Google Scholar 

  • Gengenbach, B.G., Donovan, C.M.: Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures.-Proc. nat. Acad. Sci. USA 74: 5113–5117, 1977.

    Google Scholar 

  • Holford, P., Newbury, H.J.: The effects of antibiotics and their breakdown products on the in-vitro growth of Antirrhinum majus.-Plant Cell Rep. 11: 93–96, 1992.

    Google Scholar 

  • Johnson, J.L., Emino, E.R.: Tissue culture propagation in the cactaceae.-Cactus Succulent J. 51: 275–277, 1979.

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.

    Google Scholar 

  • Maliga, P.S., Breznovits, A., Martion, L.: Streptomycin resistant plants from callus culture of haploid tobacco.-Nature 244: 29–30, 1973.

    Google Scholar 

  • Mathews, H.: In vitro responses of Brassica juncea and Vigna radiata to the antibiotic kanamycin.-Ann. Bot. 62: 671–675, 1988.

    Google Scholar 

  • Mathias, R.J., Boyd, L.A.: Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L. EM. Thell).-Plant Sci. 46: 217–223, 1986.

    Google Scholar 

  • Mathias, R.J., Mukasa, C.: The effect of cefotaxime on the growth and regeneration of callus from four varieties of barley (Hordeum vulgare L.).-Plant Cell Rep. 6: 454–457, 1987.

    Google Scholar 

  • Meredith, C.P.: Response of cultured tomato cells to aluminium.-Plant Sci. Lett. 12: 17–24, 1978.

    Google Scholar 

  • Minocha, S.C., Mehra, P.N.: Nutritional and morphogenetic investigations on callus cultures of Neomammillaria prolifera Miller (Cactaceae).-Amer. J. Bot. 61: 168–173, 1974.

    Google Scholar 

  • Mukherji, S., Biswas, A.K.: Penicillin stimulating growth and metabolism in seedling of rice (Oryza sativa).-Can. J. Bot. 63: 1150–1156, 1985.

    Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Google Scholar 

  • Nakano, M., Mii, M.: Antibiotics stimulate somatic embryogenesis with plant growth regulators in several Dianthus cultivars.-J. Plant Physiol. 141: 721–725, 1993.

    Google Scholar 

  • Owens, L.D.: Kanamycin promoted morphogenesis of plant tissues.-Plant Sci. Lett. 16: 225–230, 1979.

    Google Scholar 

  • Pius, J., George, G, Eapen, S., Rao, P.S.: Enhanced plant regeneration in pearl millet (Pennisetum americanum) by ethylene inhibitors and cefotaxime.-Plant Cell Tissue Organ Cult. 32: 91–96, 1993.

    Google Scholar 

  • Pollock, K., Barfield, D.G., Shields, R.: The toxicity of antibiotics to plant cell cultures.-Plant Cell Rep. 2: 36–39, 1983.

    Google Scholar 

  • Reed, B,M., Buckley, P.M., De Wilde, T.N.: Detection and eradication of endophytic bacteria from micropropagated mint plants.-In Vitro cell. develop. Biol. 31: 53–57, 1995.

    Google Scholar 

  • Sarma, K.S., Evans, N.E., Selby, C.: Effect of carbenicillin and cefotaxime on somatic embryogensis of Sitka spruce (Picea sitchensis (Bong.) Carr.).-J. exp. Bot. 46: 1779–1781, 1995.

    Google Scholar 

  • Teng, W.L., Nicholson, L.: Pulse treatment of penicillin-G and streptomycin minimise internal infections and have post-treatment effects on the morphogenesis of ginseng root culture.-Plant Cell Rep. 16: 531–535, 1997.

    Google Scholar 

  • Thurston, K.C., Spencer, S.J., Arditti, J.: Phytotoxicity of fungicides and bacteriosides in orchid culture media.-Amer. J. Bot. 66: 825–835, 1979.

    Google Scholar 

  • Umbeck, P.F., Gengenbach, B.G.: Treptomycin and other inhibitors as selection agents in corn tissue cultures.-Crop Sci. 23: 717–719, 1983.

    Google Scholar 

  • Valobra, C.P., James, D.J.: In vitro shoot regeneration from leaf discs of Betula pendula "Dalecarlica" EM 85.-Plant Cell Tissue Organ Cult. 21: 51–54, 1990.

    Google Scholar 

  • Wakhlu, A.K., Bhau, B.S.: Callus formation and regeneration of Coryphantha elephantidens (Lem.) Lem. from callus cultures raised from tubercles.-In Vitro cell. develop. Biol. Plant. 36: 211–214, 2000.

    Google Scholar 

  • Yepes, L.M., Aldwinckle, H.S.: Factors that affect leaf regeneration efficiency in apple, and effect of antibiotics in morphogenesis.-Plant Cell Tissue Organ Cult. 37: 257–296, 1994.

    Google Scholar 

  • Young, P.M., Hutchins, A.S., Canfield, M.L.: Use of antibiotics to control bacteria in shoot cultures of woody plants.-Plant Sci. Lett. 34: 203–209, 1984.

    Google Scholar 

  • Zhang, Q., Wiskich, J.T., Woole, K.I.: Respiratory activities in chloromphenicol-treated tobacco cells.-Physiol. Plant. 105: 224–232, 1999.

    Google Scholar 

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Botany, University of Jammu, Jammu -, 180 006, Jammu & Kashmir, India

    B.S. Bhau & A.K. Wakhlu

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  1. B.S. Bhau
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  2. A.K. Wakhlu
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Bhau, B., Wakhlu, A. Effect of Some Antibiotics on the In Vitro Morphogenetic Response from Callus Cultures of Coryphantha Elephantidens. Biologia Plantarum 44, 19–24 (2001). https://doi.org/10.1023/A:1017905917971

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