Skip to main content
Log in

Inheritance and linkage study of isozyme loci and morphological traits in red clover

  • Published:
Euphytica Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Information on inheritance of isozymes and linkage maps in Trifolium pratense L is limited. Genetic analysis of 18 isozyme systems and three morphological traits (white vs. pink flower, present vs. absent leaf marker and green vs. sun-red stem) was conducted. Six loci of four isozymes had simple Mendelian inheritance. Joint segregation analyses among 8 isozyme loci and 3 morphological gene markers (55 pair-wise combinations) resulted in the assignment of one linkage group. Two morphological markers coding for flower (c) and stem (gs) color were found to be in the same linkage group based on the analyses of two segregating families. The recombination values of the two progenies for c-gs were 0.079 and 0.113. No linkage was found between any of the isozyme loci and the morphological traits.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Acquaah, G, 1992. Practical Protein Electrophoresis for Genetic Research. Dioscorides Press. Portland, Oregon.

    Google Scholar 

  • Frei, O.M., C.W. Stuber & M.M. Goodman, 1986. Yield manipulation from selection on allozyme genotypes in a composite of elite corn lines. Crop Sci 26: 917–927.

    Article  Google Scholar 

  • Hagen, M.J. & J.L. Hamrick, 1998. Genetic variation and population genetic structure in Trifolium pratense. J Hered 89: 178–181.

    Article  PubMed  CAS  Google Scholar 

  • Henn, G., A.W.H. Neitz & A.I. Louw, 1992. Identification of tomato cultivars (Lycopersicon esculentum) by polyacrylamide isoelectric focusing. Euphytica 62: 77–82.

    Article  CAS  Google Scholar 

  • Hickey, R.J., M.A. Vincent & S.I. Guttman, 1991. Genetic variation in running Buffalo clover (Trifolium stoloniferum, Fabaceae). Conservation Biology 5: 309–316.

    Article  Google Scholar 

  • Huang, H., F. Dane & J.D. Norton, 1994. Genetic analysis of 11 polymorphic isozyme loci in chestnut species and characterization of chestnut cultivars by multi-locus allozyme genotypes. J Amer Soc Hort Sci 119: 840–849.

    CAS  Google Scholar 

  • Jones, T.W.A, 1991. Inheritance of a mutation which suppresses flowering in red clover. J Exp Bot 42: 1589–1594.

    Google Scholar 

  • Knerr, L.D. & J.E. Staub, 1992. Inheritance and linkage relationships of isozyme loci in cucumber (Cucumis sativus L.). Theor Appl Genet 84: 217–224.

    Article  CAS  Google Scholar 

  • Mulcahy, D.L., R.W. Robinson, M. Ihara & R. Kesseli, 1981. Gametophytic transcription for acid phosphatases in pollen of Cucurbita species hybrids. J Hered 72: 353–354.

    CAS  Google Scholar 

  • Murphy, R.W., J.W. Sites, D.G. Buth & C.H. Haufler, 1996. Protein: isozyme electrophoresis. In: D.M. Hillis, C. Moritz & B.K. Mable (Eds.), Molecular Systematics. Second edition, pp. 51–120. Sinauer Associates, Inc, Sunderland, MA, USA.

    Google Scholar 

  • Quesenberry K.H., R.R. Smith, N.L. Taylor, D.D. Baltensperger & W.A. Parrott, 1991. Genetic nomenclature in clovers and specialpurpose legumes I. Red and white clover. Crop Sci 31: 861–867.

    Article  Google Scholar 

  • Stuber, C.W., M.D. Edwards & J.F. Wendel, 1987. Molecular marker-facilitated investigations of quantitative trait loci in maize. II. Factors influencing yield and its component traits. Crop Sci 27: 639–648.

    Article  Google Scholar 

  • Stuber, C.W., J.F. Wendel, M.M. Goodman & J.S.C. Smith, 1988, Technique and Scoring Procedures for Starch Gel Electrophoresis of Enzymes from Maize (Zea mays L.). Technical Bulletin 286. N. Carolina State Univ., Raleigh, NC.

    Google Scholar 

  • Suiter, K.A., J.F. Wendel & J.S. Case, 1983. LINKAGE-1: A PASCAL computer program for the detection and analysis of genetic linkage. J Hered 74: 203–204.

    PubMed  CAS  Google Scholar 

  • Taylor, N.L., 1980, Clovers. In W.R. Fehr & H.H. Hadley (Eds.), Hybridization of Crop Plants, pp. 261–272. ASA, CSSA, Madison, WI.

    Google Scholar 

  • Taylor, N.L., 1982, Registration of gene marker germplasm for red clover (Reg. no. GP1 to GP11). Crop Sci 22: 1269.

    Article  Google Scholar 

  • Taylor, N.L. & R.R. Smith, 1979. Red clover breeding and genetics. Adv. in Agronomy 31: 125–155.

    Article  Google Scholar 

  • Weeden, N.F, 1989. Applications of isozymes in plant breeding. Plant Breeding Reviews 6: 11–50.

    CAS  Google Scholar 

  • Wendel, J.F. & N.F. Weeden, 1989. Visualization and interpretation of plant isozymes. In: D.E. Soltis & P.S. Soltis (Eds.), Isozymes in Plant Biology, pp. 5–45. Dioscorides Press, Portland, Oregon.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xie, C., Mosjidis, J. Inheritance and linkage study of isozyme loci and morphological traits in red clover. Euphytica 119, 253–257 (2001). https://doi.org/10.1023/A:1017599612114

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1017599612114

Navigation