Skip to main content
Log in

An Automated Approach to Luminescence Lifetime and Intensity Titrations

  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

An apparatus is described for the automated collection of luminescence emission decay curves over a wide range of analyte concentrations. The decay curves allow for determination of the excited-state lifetime or calculated steady-state intensity of a luminophore as a function of the analyte concentration. The data presented here demonstrate the use of the apparatus for pH titrations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  1. J. E. Whitaker, R. P. Haugland, and F. G. Prendergast (1991) Anal. Biochem. 194, 330-334.

    Google Scholar 

  2. J. Price, W. Xu, and J. N. Demas (1998) Anal. Chem. 70, 265-270.

    Google Scholar 

  3. H. Szmacinski and J. R. Lakowicz (1993) Sensors Actuat. B 11, 133-143.

    Google Scholar 

  4. R. B. Thompson and J. R. Lakowicz (1993) Anal. Chem. 65, 853-856.

    Google Scholar 

  5. G. Liebch, I. Klimant, B. Frank, G. Holst, and O. S. Wolfbeis (2000) Appl. Spectrosc. 54, 548-559.

    Google Scholar 

  6. U. Kosch, I. Klimant, and O. S. Wolfbeis (1999) Anal. Chem. 70, 3892-3897.

    Google Scholar 

  7. G. Neurauter, I. Klimant, and O. S. Wolfbeis (1999) Anal. Chim. Acta 382, 67-75.

    Google Scholar 

  8. J. R. Lakowicz, H. Szmacinski, and M. L. Johnson (1992) J. Fluoresc. 2, 47-62.

    Google Scholar 

  9. G. Grynkiewicz, M. Poenie, and R. Y. Tsien (1985) J. Biol. Chem. 260, 3440-3450.

    Google Scholar 

  10. E. U. Akkaya and J. R. Lakowicz (1993) Anal. Biochem. 213, 285-289.

    Google Scholar 

  11. M. Shortreed, R. Kopelman, and M. Kuhn (1996) Anal. Chem. 68, 1414-1418.

    Google Scholar 

  12. O. J. Rolinski and D. J. S. Birch (1999) Measure. Sci. Tech. 10, 127-137.

    Google Scholar 

  13. D. J. S. Birch, O. J. Rolinski, and D. Hatrick (1996) Rev. Sci. Instrum. 67, 2732-2737.

    Google Scholar 

  14. Labview 4.0 (1996) National Instruments Corp., Austin, TX.

  15. L. Sacksteder, A. P. Zipp, E. A. Brown, J. Streich, J. N. Demas, and B. A. DeGraff (1990) Inorg. Chem. 29, 4335-4340.

    Google Scholar 

  16. L. Sacksteder, J. N. Demas, and B. A. DeGraff (1989) Inorg. Chem. 28, 1787-1792.

    Google Scholar 

  17. Y. Clarke, W.-Y. Xu, J. N. Demas, and B. A. DeGraff (2000) Anal. Chem. 72, 3468-3475.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to J. N. Demas.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mack, N.H., Bare, W.D., Xu, W. et al. An Automated Approach to Luminescence Lifetime and Intensity Titrations. Journal of Fluorescence 11, 113–118 (2001). https://doi.org/10.1023/A:1016677401822

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1016677401822

Navigation