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Adhesion and morphology of fibroblastic cells cultured on different polymeric biomaterials

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Abstract

Cell adhesion is influenced by the physical and chemical characteristics of the materials used as substrate for cell culturing. In this work, we evaluated the influence of the morphological and chemical characteristics of different polymeric substrates on the adhesion and morphology of fibroblastic cells. Cell growth on poly (L-lactic acid) [PLLA] membranes and poly(2-hydroxy ethyl methacrylate) [polyHEMA], poly(2-hydroxy ethyl methacrylate)-cellulose acetate [polyHEMA-CA] and poly(2-hydroxy ethyl methacrylate)-poly(methyl methacrylate-co-acrylic acid) [polyHEMA-poly(MMA-co-AA)] hydrogels of different densities and pore diameters was examined. Cells adhered preferentially to more negativaly charged substrates, with polyHEMA hydrogels being more adhesive than the other substractes. The pores present in PLLA membranes did not interfere with adhesion, but the cells showed a distinctive morphology on each membrane.

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References

  1. P. TÖrmÄlÄ, T. Pohjonen and P. Rokkanen, Proc. Instn. Mech. Engrs. 212(H) (1997) 101-111.

    Google Scholar 

  2. J. A. Hubbell, Biotechnology 13 (1995) 565-576.

    PubMed  Google Scholar 

  3. M. J. Lyndon, T. W. Minett and B. J. Tighe, Biomaterials 6 (1985) 396-402.

    PubMed  Google Scholar 

  4. A. Van Sliedregt, A. M. Radder, K. De Groot and C. A. Van Blitterswijk, J. Mater. Sci. Mater. Med. 3 (1992) 365-370.

    Google Scholar 

  5. A. Van Sliedregt, K. De Groot and C. A. Van Blitterswijk, J. Mater. Sci. Mater. Med. 4 (1993) 213-218.

    Google Scholar 

  6. P. Robert, J. Mauduit, R. M. Frank and M. Vert, Biomaterials 14 (1993) 353-358.

    PubMed  Google Scholar 

  7. A. Van Sliedregt, J. A. Van Loon, J. Van Der Brink, K. De Groot K and C. A. Van Blitterswijk, ibid. 15 (1994) 251-256.

    PubMed  Google Scholar 

  8. R. E. Kesting, “Synthetic Polymeric Membranes: A Strutural Perspective”, 2nd edn, (John Wiley and Sons, 1985).

  9. K. H. Lam, I. Nieuwenhuis, I. Molenaar, H. Esselbrugge, J. Feijen, J. Dijkstra and J. M. Schakenrrad, J. Mater. Sci. Mater. Med. 5 (1994) 181-189.

    Google Scholar 

  10. C. H. Schugens, C. Grandfils, R. Jerome, P. H. Teyssie, P. Delree, D. Martin, B. Malgrange and G. Moonen, J. Biomed. Mater. Res. 29 (1995) 1349-1362.

    PubMed  Google Scholar 

  11. D. J. Mooney, D. F. Baldwin, P. S. Suh, J. P. Vacanti and R. Langer, Biomaterials 17 (1996) 1417-1422.

    PubMed  Google Scholar 

  12. A. G. A. Coombes and J. D. Heckman, ibid. 13 (1992) 297-307.

    PubMed  Google Scholar 

  13. V. Kudela, in “Encyclopedia of Polymer Science and Engineering”, edited by I. K. Jacqueline 2nd edn. (Wiley Interscience, 1990) pp. 783-807.

  14. J. Folkman and A. Moscon, Nature 273 (1978) 345-349.

    PubMed  Google Scholar 

  15. T. A. Horbett, J. J. Waldburger, B. D. Ratner and A. S. Hoffman, J. Biomed. Mater. Res. 22 (1998) 383-404.

    Google Scholar 

  16. K. Smetana, ibid. 24 (1990) 463-470.

    PubMed  Google Scholar 

  17. J. H. Lee, H. W. Jung, I. K. Kang and H. B. Lee, Biomaterials 15 (1994a) 705-711.

    PubMed  Google Scholar 

  18. J. H. Lee, J. W. Lee, G. Khang and H. B. Lee, ibid. 18 (1997) 351-358.

    PubMed  Google Scholar 

  19. S. M. Malmonge, C. A. C. Zavaglia, A. R. Santos Jr. and M. L. F. Wada, Rev. Bras. Eng. Biomed. 15 (1999) 49-54.

    Google Scholar 

  20. S. M. Malmonge, C. A. C. Zavaglia and W. D. Belangero, Braz. J. Med. Biol. Res. 33 (2000) 307-312.

    PubMed  Google Scholar 

  21. C. J. Kirkpatric, Regulatory Affair 4 (1992) 13-32.

    Google Scholar 

  22. ISO 10993-5:1992 (E) Biological evaluation of medical devices — Part 5 — tests for cytotoxicity: in vitro methods.

  23. T. Mosmann, J. Immunol. Meth. 65 (1993) 55-65.

    Google Scholar 

  24. R. J. Pelham, Y. L. Wang, Proc. Natl. Acad. Sci. USA 94 (1997) 13661-13665.

    PubMed  Google Scholar 

  25. S. C. Lee, M. Shea, M. A. Battle, K. Kozitza, E. Ron, T. Turek, R. G. Schaub and W. C. Hayesh, J. Biomed. Mater. Res. 28 (1994b) 1149-1156.

    PubMed  Google Scholar 

  26. R. C. Culp, Curr. Top. Membr. Transp. 2 (1978) 327-396.

    Google Scholar 

  27. F. Grinnell and M. K. Feld, J. Biomed. Mater. Res. 15 (1981) 363-381.

    PubMed  Google Scholar 

  28. C. B. Lombello, S. M. Malmonge and M. L. F. Wada, J. Mater. Sci. Mater. Med. 11 (2000b) 541-546.

    PubMed  Google Scholar 

  29. J. Hambleton, Z. Schwartz, A. Khare, S. W. Windeler, M. Luna, B. P. Brooks, D. D. Dean and B. D. Boyan, J. Orthop. Res. 12 (1994) 542-552.

    PubMed  Google Scholar 

  30. G. A. Hutcheon, S. Downes, M. C. Davies, J. Mater. Sci. Mater. Med. 9 (1998) 815-818.

    PubMed  Google Scholar 

  31. K. Smetana, Biomaterials 14 (1993) 1046-1050.

    PubMed  Google Scholar 

  32. B. K. Mann, A. T. Tsai, T. Scott-Burden and J. L. West, Biomaterials 20 (1999) 2281-2286.

    PubMed  Google Scholar 

  33. A. R. Jr Santos, S. H. Barbanti, E. A. R. Duek, H. Dolder, R. S. Wada and M. L. F. Wada, Artif. Organs 25 (2001) 7-13.

    PubMed  Google Scholar 

  34. R. A. Zoppi, S. Contant, E. A. R. Duek, F. R. Marques, M. L. F. Wada and S. P. Nunes, Polymer 40 (1999) 3275-3289.

    Google Scholar 

  35. C. B. Lombello, S. M. Malmonge and M. L. F. Wada, Cytobios 101 (2000a) 115-122.

    PubMed  Google Scholar 

  36. K. D. Chesmel, J. Black, J. Biomed. Mater. Res. 29 (1995) 1089-1099.

    PubMed  Google Scholar 

  37. A. R. Jr Santos, M. L. F. Wada, Cytobios 99 (1999) 159-171.

    PubMed  Google Scholar 

  38. S. S. Maria and M. L. F. Wada, In vitro Cell Dev. Biol. 33 (1997) 748-750.

    Google Scholar 

  39. M. L. F. Wada and B. C. Vidal, Cytobios 67 (1991) 101-109.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Cell Biology, Institute of Biology, Campinas, SP, Brazil

    C. B. Lombello, A. R. Santos Jr. & M. L. F. Wada

  2. Labiomec, DDPP/Center of Technology, Campinas, SP, Brazil

    S. M. Malmonge

  3. Department of Materials Engineering, Faculty of Mechanical Engineering, State University of Campinas (UNICAMP), Campinas, SP, Brazil

    S. H. Barbanti

  4. Department of Physiological Sciences, College of Biological Sciences, Pontificial Catholic University of São Paulo (PUC-SP), Sorocaba, SP, Brazil

    E. A. R. Duek

Authors
  1. C. B. Lombello
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  3. S. M. Malmonge
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  5. M. L. F. Wada
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  6. E. A. R. Duek
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Lombello, C.B., Santos, A.R., Malmonge, S.M. et al. Adhesion and morphology of fibroblastic cells cultured on different polymeric biomaterials. Journal of Materials Science: Materials in Medicine 13, 867–874 (2002). https://doi.org/10.1023/A:1016552413295

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