Abstract
In order to search tissue-specific elements in the 5′-upstream promoter region, gene gun was used to transfect in vivo plasmid DNAs with varying lengths of truncated ovalbumin promoter fused to the CAT reporter gene to the oviduct and liver of laying hens. The results indicated that in the oviduct, consistently high reporter gene expression was observed irrespective of the length of the truncated ovalbumin gene promoters, whereas in the liver the ovalbumin promoter extending from -3200 to +8 bp suppressed substantially the reporter gene expression compared with consistently high gene expression obtained by the ovalbumin promoters from -2800 to +8 bp or shorter length. It was concluded, therefore, that a tissue-specific silencer-like element might reside most likely in the ovalbumin gene promoter region between -3200 and -2800 bp which represses the ovalbumin gene transcription in the liver, but not in the oviduct of laying hens.
Similar content being viewed by others
References
Muramatsu T, Sanders MM: Regulation of ovalbumin gene expression. Poult Avian Biol Rev 6: 107–123, 1995
Ciejek EM, Tsai M-J, O'Malley BW: Actively transcribed genes are associated with the nuclear matrix. Nature 306: 607–609, 1983
Kaye JS, Pratt-Kaye S, Bellard M, Dretzen G, Bellard F, Chambon P: Steroid hormone dependence of four DNase I-hypersensitive regions located within the 7000 bp 57-flanking segment of the ovalbumin gene. EMBO J 5: 277–285, 1986
Dierich A, Gaub P-P, LePennec J-P, Astinotti D, Chambon P: Cell-specificity of the chicken ovalbumin and conalbumin promoters. EMBO J 6: 2305–2312, 1987
Sanders MM, McKnight GS: Positive and negative regulatory elements control the steroid-responsive ovalbumin promoter. Biochemistry 27: 6550–6557, 1988
Haecker SA, Muramatsu T, Sensenbaugh KR, Sanders MM: Repression of the ovalbumin gene involves multiple negative elements including a ubiquitous transcriptional silencer. Mol Endocrinol 9: 1113–1126, 1995
Love J, Gribbin C, Mather C, Sang H: Transgenic birds by DNA microinjection. Bio/Technology 12: 60–63, 1994
Dzau VJ, Morishita R, Gibbons GH: Gene therapy for cardiovascular disease. Trends Biotechnol 11: 205–210, 1993
Findeis MA, Merwin JR, Spitalny GL, Chiou HC: Targeted delivery of DNA for gene therapy via receptors. Trends Biotechnol 11: 202–205, 1993
Rosenblum CI, Chen HY: In ovo transfection of chicken embryos embryos using cationic liposomes. Transgenic Res 4: 192–198, 1995
Muramatsu T, Shibata O, Ohmori Y, Okumura J: In vivo electroporation: A convenient method for gene transfer to testicular cells in mice. Anim Sci Technol (Jpn) 67: 975–982, 1996
Muramatsu T, Shibata O, Okumura J: Effect of self-replication DNA sequences of Epstein-Barr virus on the expression of a foreign gene transfected in vivo to the mouse testis. Anim Sci Technol (Jpn) 68: 650–653, 1997
Klein TM, Arentzen R, Lewis PA, Fitzpatrick-McElligott S: Transformation of microbes, plants and animals by particle bombardment. Bio/Technology 10: 286–291, 1992
Sambrook J, Fritsch EF, Maniatis T: In: Molecular Cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989, vol 3, pp 16.66–16.67
Pothier F, Ouellet M, Julien J-P, Guerin SL: An improved CAT assay for promoter analysis in either transgenic mice or tissue culture cells. DNA Cell Biol 11: 83–90, 1992
SAS-Institute (1985) SAS User's Guide: Statistics. Version 5 ed., SAS Institute, Cary, NC
Stumph WE, Batz M, Beattie WG, Tsai M-J, O'Malley BW: Characterization of deoxyribonucleic acid sequences at the 5′ ?and 3′ borders of the 100 kilobase pair ovalbumin gene domain. Biochemistry 22: 306–315, 1983
Gaub M-P, Dierich A, Astinotti D, Touitou I, Chambon P: The chicken ovalbumin promoter is under negative control which is relieved by steroid hormones. EMBO J 6: 2313–2320, 1990
Muramatsu T, Shibata O, Ryoki S, Okumura J: Foreign gene expression in the mouse testis by localized in vivo gene transfer. Biochem Biophys Res Commun 233: 45–49, 1997
Kato S, Tora L, Yamauchi J, Masushige S, Bellard M, Chambon P: A far upstream estrogen response element of the ovalbumin gene contains several half-palindromic 5′-TGACC-3′ ?motifs acting synergistically. Cell 68: 731–742, 1992
Luckow B: CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucl Acids Res 15: 5490, 1987
Williams RS, Johnston SA, Reidy M, de Vit MJ, McElligott SG, Sanford JC: Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. Proc Natl Acad Sci USA 88: 2726–2730, 1991
Foster DO, Frydman ML: Comparison of microspheres and 86Rb+ as tracers of the distribution of cardiac output in rats indicates invalidity of 86Rb+-based measurements. Can J Physiol Pharmacol 56: 97–109, 1978
Alam J, Cook JL: Reporter genes: Application to the study of mammalian gene transcription. Anal Biochem 188: 245–254, 1990
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Muramatsu, T., Imai, T., Park, HM. et al. Gene gun-mediated in vivo analysis of tissue-specific repression of gene transcription driven by the chicken ovalbumin promoter in the liver and oviduct of laying Hens. Mol Cell Biochem 185, 27–32 (1998). https://doi.org/10.1023/A:1016507900718
Issue Date:
DOI: https://doi.org/10.1023/A:1016507900718