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The Lower-Generation Polypropylenimine Dendrimers Are Effective Gene-Transfer Agents

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Abstract

Objective. To evaluate polypropylenimine dendrimers (generations 1-5: DAB 4, DAB 8, DAB 16, DAB 32, and DAB 64) as gene delivery systems.

Methods. DNA binding was evaluated by measuring the reduced fluorescence of ethidium bromide, and molecular modelling of dendrimer-DNA complexes also was performed. Cell cytotoxicity was evaluated against the A431 cell line using the MTT assay. In vitro transfection was evaluated against the A431 cell line using the β-galactosidase reporter gene and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulphate (DOTAP) served as a positive control.

Results. Molecular modeling and experimental data revealed that DNA binding increased with dendrimer generation. Cell cytotoxicity was largely generation dependent, and cytotoxicity followed the trend DAB 64 > DAB 32 > DAB 16 > DOTAP > DAB 4 > DAB 8, whereas transfection efficacy followed the trend DAB 8 = DOTAP = DAB 16 > DAB 4 > DAB 32 = DAB 64.

Conclusion. The generation 2 polypropylenimine dendrimer combines a sufficient level of DNA binding with a low level of cell cytoxicity to give it optimum in vitro gene transfer activity.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, G4 0NR, Scotland

    Bernd H. Zinselmeyer, Simon P. Mackay & Ijeoma F. Uchegbu

  2. Cancer Research Campaign Department of Medical Oncology, University of Glasgow, Switchback Road, Glasgow, G61 1BD, Scotland

    Bernd H. Zinselmeyer & Andreas G. Schatzlein

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  1. Bernd H. Zinselmeyer
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  2. Simon P. Mackay
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  3. Andreas G. Schatzlein
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  4. Ijeoma F. Uchegbu
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Correspondence to Ijeoma F. Uchegbu.

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Zinselmeyer, B.H., Mackay, S.P., Schatzlein, A.G. et al. The Lower-Generation Polypropylenimine Dendrimers Are Effective Gene-Transfer Agents. Pharm Res 19, 960–967 (2002). https://doi.org/10.1023/A:1016458104359

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