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Genetic analysis of resistance to barley scald (Rhynchosporium secalis) in the Ethiopian line `Abyssinian' (CI668)

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Abstract

A doubled haploid barley (Hordeum vulgare L.) population from a cross between the cultivar `Ingrid' and the Ethiopian landrace `Abyssinian' was mapped by AFLP, RFLP, SSR and STS markers and tested for resistance to isolates`4004', `2', `16-6', `17', `22' and `WRS 1872' of Rhynchosporium secalis (Oudem.) J.J. Davis, the causal agent of leaf scald. Resistance tests were conducted on parents, DH-lines, a near-isogenic line of `Abyssinian' (NIL) into `Ingrid', and an F2 population descended from the same F1 plants as the DHs. The DH population segregated for at least two major R. secalis resistance QTL. All isolates tested identified a major QTL on chromosome 3 (3H) associated with R. secalis resistance, in a 4 cM support interval between the co-segregating markers Bmac0209/Falc666 and MWG680. The QTL was linked with the markers Falc666 (2.3 cM), YLM/ylp (0.3 cM), MWG680 (1.7 cM), cttaca2 (2.5 cM) and agtc17 (9.8 cM). The second QTL was located on chromosome 1 (7H).However, this QTL was only detected by one isolate and was located in an interval of 16 cM in the distal part of the chromosome. At this QTL the allele for improved scald resistance originated from the parent `Ingrid'. There were a number of minor QTL on chromosomes 2 (2H), 4 (4H) and 6 (6H) that were not repeatable either across replications or analysis methods. The importance of checking QTL-models by cross-validation is stressed.

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Authors and Affiliations

  1. Department of Horticulture and Crop Sciences, Agricultural University of Norway, P.O. Box 5022, N-1432, Ås, Norway

    S. Grønnerød, A.G. Marøy & A. Bjørnstad

  2. Department of Plant Breeding Research, Swedish University of Agricultural Sciences, S-75007, Uppsala, Sweden

    J. MacKey

  3. Cereal Research Centre, Agriculture Canada, Winnipeg, Canada

    A. Tekauz & G.A. Penner

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  1. S. Grønnerød
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  2. A.G. Marøy
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  3. J. MacKey
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  4. A. Tekauz
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  5. G.A. Penner
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  6. A. Bjørnstad
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Grønnerød, S., Marøy, A., MacKey, J. et al. Genetic analysis of resistance to barley scald (Rhynchosporium secalis) in the Ethiopian line `Abyssinian' (CI668). Euphytica 126, 235–250 (2002). https://doi.org/10.1023/A:1016368503273

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