Abstract
The effect of multiple somatostatin (SRIH-14) treatment on the pituitary gonadotrophs, follicle stimulating harmone (FSH) and luteinizing harmone (LH), and ovaries of adult female Wistar rats was examined. Females received two 20 μg/100 g body wt. doses daily subcutaneously, for five consecutive days. FSH and LH cells were studied using a peroxidase–antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate changes in the number per unit area (mm2), cell volume and volume densities of LH- and FSH-immunoreactive cells. Ovaries were analysed by simple point counting of follicles and corpora lutea. Follicles were divided by size according to the classification of Gaytán and Osman. Morphometric and stereological analysis of the pituitary showed that the number, volume and the volume density of FSH- and LH-immunoreactive cells were decreased after multiple SRIH-14 treatment, particularly in the latter. In the ovary, SRIH-14 induced decreases in the number of healthy follicles in all phases of folliculogenesis and corpora lutea, but the large antral follicle stage was most affected. The number of atretic follicles was increased. It can be concluded that multiple SRIH-14 treatment markedly inhibited LH cells, but affected FSH cells as well. In the ovary, SRIH-14 acted by inhibiting folliculogenesis and enhancing atretic processes.
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Adashi EY (1993) Growth hormone as a gonadotropin. In: Bouchard P, Caraty A, Coeling Bennink HJT, Pavlou SN, eds. GnRH, GnRH Analogs Gonadotropins and Gonadal Peptides. New York: Parthenon Publishing Group, pp. 569–591.
Andreani CL, Lazzarin N, Peirro E, Lanzone A, Mancuso S (1995) Somatostatin action on rat ovarian steroidogenesis. Human Reprod 10: 1968–1973.
Artini PG, De Micheroux AA, D'Ambrogio G (1996) Growth hormone with gonadotropins in ovulation induction. J Endicrinol Invest 19:763–779.
Baumeister H, Kreuzer OJ, Roosterman D, Schafer J, Meyerhof W (1998) Cloning, expression, pharmacology and tissue distribution of the mouse somatostatin receptor subtype 5. J Neuroendocrinol 10:283–290.
Brazeau P, Vale W, Burgus R, Ling N, Butcher M, Rivier J, Guillemin R (1973) Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science 179:77–83.
Eckery DC, Moeller CL, Nett TM, Sawyer HR (1997) Localization and quantification of binding sites for follicle-stimulating hormone, luteinizing hormone, growth hormone, and insulin-like growth factor I in sheep ovarian follicles. Biol Reprod 51: 507–513.
Gaytán F, Morales C, Bellido C, Aguilar E, Sánchez-Criado JE (1996) Proliferative activity of different ovarian compartments in cycling rats estimated by the 5–bromo-deoxyuridine technique. Biol Reprod 54: 1356–1365.
Greenwald GS, Roy SK(1994) Follicular development and its control. In: Knobil E, Neill JD, eds. The Physiology of Reproduction. New York: Raven Press, pp. 629–724.
Holst N, Jacobsen MB, Haug E, Tanbo T, Åbyholm T (1995) Somatostatin in physiological concentrations inhibits basal and enhances luteinizing hormone stimulated progesterone release from human granulosa luteal cells. Human Reprod 10: 1363–1366.
Kobayashi J, Mizunuma H, Kikuchi N, Liu X, Andoh K, Abe Y, Yokota H, Yamada K, Ibuki Y, Hagiwara H (2000) Morphological assessment of the effects of growth hormone on preantral follicles from 11–day-old mice in an in vitro culture system. Biochem Biophys Res Commun 268: 36–41.
Krulich L, Dhariwal APS, McCann SM(1968) Stimulatory and inhibitoryeffects of purified hypothalamic extracts on growth hormone release from rat pituitary in vitro. Endocrinology 83: 783–790.
Lamberts SWJ (1988) The role of somatostatin in the regulation of anterior pituitary hormone secretion and use of its analogs in the treatment of human pituitary tumors. Endocrine Rev 9: 417–436.
Lovren M (1986) Regulation of ovaries development in animals treated with gonadal steroids. Acta Vet 36: 236–276.
Lovren M, Pantić V (l987) Ovarian follicle development and atresia in rats neonatally treated with estrogen. Acta Vet 37: 81–92.
Lovren M, Pantić V (1989) Reaction of the ovarian tissues in rats treated with estrogen during postnatal development. Acta Vet 39:71–80.
Lovren M, Sekulić M, MiloŠević V, Radulović N (1998) Effects of somatostatins on gonadotrophic cells in female rats. Acta Histochem 100: 329–335.
Masini MA, Sturla M, Uva B (1999) Somatostatin in the ovary of an African lungfish (Protopterus annectens): An in situ hybridisation, immunohistochemical, and autoradiographical study. Gen Comp Endocrinol 114: 287–292.
McCann SM (1982) Physiology and pharmacology of LHRH and somatostatin. Annu Rev Pharmacol Toxicol 22: 491–515.
McIntyre HD, Maréchal DJ, Dérby GP, Mathieu AG, Hézée-Hagelstein MT, Franchimont PP (1992) Immunoreactive somatostatin in the rat ovary. Acta Endocrinol 126: 553–558.
Monniaux D, Huet C, Besnard N, Clément F, Bosc M, Pisselet C, Monget P, Mariana JC (1997) Follicular growth and ovarian dynamics in mammals. J Reprod Fertil 51: 3–23.
Mori T, Saito H, Ohno Y, Irahara M, Hosoi E, Saito S (1984) Evidence for existence of somatostatin like immunoreactivity with molecular heterogeneity in porcine ovaries. Acta Endocrinol 106: 254–259.
O'Carroll AM, Krempels K (1995) Widespread distribution of somatostatin messenger ribonucleic acid in rat pituitary. Endocrinology 136:5224–5227.
Osman P (1985) Rate and course of atresia during follicular development in the adult cyclic rat. J Reprod Fertil 73: 261–270.
Patel YC (1990) Somatostatin. In: Becker K, ed. Principles and Practice of Endocrinology and Metabolism. Philadelphia:Lippincott, pp. 1297–1301.
Patel YC (1992) General aspects of the biology and function of somatostatin. In: Weil C, Muller EE, Thorner MO, eds. Basic and Clinical Aspects of Neuroscience, Somatostatin. Vol. 4, New York: Springer,pp. 1–16.
Prelević MG, Wurzburger IM, Balint-Perić Lj, NošIć JS (1990) Inhibitory effect of sandostatin on secretion of luteinizing hormone and ovarian steroids in polycystic ovary sindrome. Lancet 336: 900–903.
Rajkumar K, Kerr DE, Kirkwood RN, Laarveld B (1992) Inhibitory action of somatostatin-14 on hormone-stimulated cyclic adenosine monophosphate induction in porcine granulosa and luteal cells. J Endocrinol 134: 297–306.
Rebuffat P, Robba C, Mazzocchi G, Nussdorfer GG (1984) Inhibitory effect of somatostatin on the growth and steroidogenic capacity of rat adrenal zona glomerulosa. J Steroid Biochem 21: 387–390. 702 N. Nestorović et al.
Reichlin S (1983) Somatostatin. Parts 1 and 2. N Engl J Med 309: 1495–1501; 1556–1563.
Reubi JC, Horisberger U, Klijn JG, Foekens JA (1991) Somatostatin receptors in differentiated ovarian tumors. Am J Pathol 138:1267–1272.
Richards JS (1980) Maturation of ovarian follicles: action and interactions of pituitary and ovarian hormones on follicular cell development. Recent Prog Horm Res 32: 477–527.
Samson WK, Aguila MC, Lumpkin MD, McCann SM (1988) Somatostatin: Recent studies on hypothalamic secretion and local bioaction. In: Negro-Villar A, Conn PM, eds. Peptide Hormones: Effects and Mechanisms of Action. Boca-Raton: FL, CRC Press, pp. 67–77.
Samuels MH, Henry P, Ridgway EC (1992) Effects of dopamine and somatostatin on pulsatile pituitary glycoprotein secretion. J Clin Endocrinol Metab 74: 217–222.
Sekulić M, Lovren M, Milošević V(2000) Somatostatin acts by inhibiting pituitary TSH cells in female rats. Acta Vet 50: 3–12.
Srkalovic G, Cai RZ, Schally AV (1990) Evaluation of receptors for somatostatin in various tumors using different analogs. J Clin Endocrinol Metab 70: 661–669.
Starčević V, Milošević V, Brkić B, Severs WB(2000) Effects of centrallyapplied somatostatin on pituitary adrenocorticotropes in female rats. Pharmacology 60: 203–207.
Sternberger LA, Hardy PH Jr, Cuculis JJ, Mayer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333.
Sy RA, Bernstein R, Chynn KY, Kourides IA (1992) Reduction in size of thyrotrophin-and gonadotrophin-secreting pituitary adenomas treated with octreotide acetate (somatostatin analog). J Clin Endocrinol Metab 74: 690–694.
Van der Meer M, De Boer JA, Hompes PG, Schoemaker J (1998) Octreotide, a somatostatin analogue, alters ovarian sensitivity to gonadotrophin stimulation as measured by the follicle stimulating threshold in polycystic ovary syndrome. Human Reprod 13:1465–1469.
Wang HS, Chard T (1999) IGFs and IGF-binding proteins in the regulation of human ovarian and endometrial function. J Endocrinol 161:1–13.
Weibel ER (1979) Stereological methods: Practical Methods of Biological Morphometry. Vol. 1, London: Academic Press, pp. 1–415.
Yu WH, Kimura M, McCann SM (1997) Effect of somatostatin on the release of gonadotropins in male rats. PSEBM 214: 83–86.
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Nestorovi'c, N., Lovren, M., Sekuli'c, M. et al. Effects of Multiple Somatostatin Treatment on rat Gonadotrophic Cells and Ovaries. Histochem J 33, 695–702 (2001). https://doi.org/10.1023/A:1016366704630
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DOI: https://doi.org/10.1023/A:1016366704630