Abstract
Cotyledonary mesophyll cells of Lycopersicon esculentummay be induced to form vessels with exogenously applied 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine. As a basis for determining the molecular events involved in the induction of competence for xylogenesis, we have determined that the entry of the exogenous plant bioregulators into the explant is through both the cut surfaces and intact epidermis; the mesophyll cells adjacent to the vascular system are the first to be induced to form vessels, appearing to be pre-programmed for the first phase of the genetic programme for vascular differentiation and so forming one set of channels of plant bioregulator flow for the determination of some of the subsequently transformed cells; and the remainder of the mesophyll cells are induced through the exogenously applied plant bioregulators entering through the surface of the cotyledon.
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Wang, L., Wyndaele, R. & Gahan, P. Induction of vessels in cotyledons of Lycopersicon esculentum by exogenously applied plant bioregulators. Plant Cell, Tissue and Organ Culture 70, 213–221 (2002). https://doi.org/10.1023/A:1016308207438
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DOI: https://doi.org/10.1023/A:1016308207438