Abstract
In the sequential courtship behavior of the German cockroach, Blattella germanica (Dictyoptera: Blattellidae), females feed on the tergal gland secretions from the male tergites. The phagostimulative components in the male secretions were composed of a complex mixture of oligosaccharides and phospholipids. Besides seven oligosaccharides previously identified, two new trisaccharides, O-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl α-D-glucopyranoside and O-α-glucopyranosyl-(1→4)-O-α-glucopyranosyl- (1→1)-myo-inositol, were characterized as the sugar components of the secretions. The activity of the sugar components was compared in combination with the lipid fraction. Individual oligosaccharides exhibited the activity either alone or synergistically with the phospholipids. A complex mixture of these primary substances in the tergal gland secretions, thus, serves as a pheromonal phagostimulant in the precopulatory behavior, strongly eliciting feeding response in the female cockroach.
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REFERENCES
Ajisaka, K. and Fujimoto, H.1990. Regioselective syntheses of trehalose-containing trisaccharides using various glycohydrolases. Carbohydr. Res. 199:227–234.
Birch, M.C.1974. Aphrodisiac pheromones in insects, pp. 115–134, inM. C. Birch (ed.). Pheromones. North-Holland, Amsterdam.
Brossut, R., Dubois, P., Rigaud, J., and Sreng, L.1975. Etude biochimique de la secretion des glandes tergales des Blattaria. Insect Biochem.5:719–732.
Dethier, V. G.1972. Feeding behaviour, pp. 208–220, inP. Barbosa and T. M. Peters (eds.). Readings in Entomology. W. B. Saunders Co., Philadelphia, Pennsylvania.
Eisner, T., Smedley, S. R., Young, D. K., Eisner, M., Roach, B., and Meinwald, J.1996. Chemical basis of courtship in a beetle (Neopyrochroa flabellata): Cantharidin as precopulatory “enticing” agent. Proc. Natl. Acad. Sci. USA93:6494–6498.
Engelmann, F.1970. Mating behavior, pp. 57–98, inF. Engelmann (ed.). The Physiology of Insect Reproduction. Pergamon Press, New York.
Gould, G. E. and Deay, H. O.1937. Notes on the bionomics of roaches inhabiting houses. Proc. Indiana Acad. Sci.47:281–284.
Gwynne, D. T.1983. Male nutritional investment and the evolution of sexual differences in Tettigoniidae and other Orthoptera, pp. 337–366, inD. T. Gwynne and G. K. Morris (eds.). Orthopteran Mating Systems: Sexual Competition in a Diverse Group of Insects. Westview Press, Boulder, Colorado.
Gwynne, D. T.1997. Glandular gifts. Sci. Am.277:46–51.
Ishii, S.1972. Sex discrimination by males of the German cockroach, Blattella germanica(L.) (Orthoptera: Blattidae). Appl. Entomol. Zool.7:226–233.
Hendrix, D. L. and Salvucci, M. E.2001. Isobemisiose: An unusual trisaccharide abundant in the silverleaf whitefly, Bemisia argentifolii. J. Insect Physiol. 47:423–432.
Koto, S., Yago, K., Zen, S., Tomonaga, F., and Shimada, S.1986. α-D-Glucosylation by 6-O-acetyl-2,3,4-tri-O-benzyl-D-glucopyranose using trimethylsilyl triflate and pyridine. Synthesis of α-maltosyl and α-isomaltosyl α-D-glucosides. Bull. Chem. Soc. Jpn. 59:411–414.
Kurimoto, M., Nishimoto, T., Nakada, T., Chaen, H., Fukuda, S., and Tsujisaka, Y.1997. Synthesis by an α-glucosidase of glycosyl-trehaloses with an isomaltosyl residue. Biosci. Biotech. Biochem. 61:699–703.
Liang, D. and Schal, C.1993. Calling behavior of the female German cockroach, Blattella germanica(Dictyoptera: Blattellidae). J. Insect Behav.6:603–614.
Nakada, T., Maruta, K., Tsusaki, K., Kubota, M., Chaen, H., Sugimoto, T., Kurimoto, M., and Tsujisaka, Y.1995. Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobactersp. Q36. Biosci. Biotech. Biochem. 59:2210–2214.
Nakada, T., Ikegami, S., Chaen, H., Kubota, M., Fukuda, S., Sugimoto, T., Kurimoto, M., and Tsujisaka, Y.1996. Purification and characterization of thermostable maltooligosyl trehalose synthase from the thermoacidophilic Archaebacterium Sulfolobus acidocaldarius. Biosci. Biotech. Biochem. 60:263–266.
Nishida, R. and Fukami, H.1983. Female sex pheromone of the German cockroach, Blattella germanica. Mem. Coll. Agric. Kyoto Univ.122:1–24.
Nojima, S., Sakuma, M., and Kuwahara, Y.1996. Polyethylene glycol film method: A test for feeding stimulants of the German cockroach, Blattella germanica(L.) (Dictyoptera: Blattellidae). Appl. Entomol. Zool.31:537–546.
Nojima, S., Nishida, R., Sakuma, M., and Kuwahara, Y.1999a. Nuptial feeding stimulants: A male courtship pheromone of the German cockroach, Blattella germanica(L.) (Dictyoptera: Blattellidae). Naturwissenschaften86:193–196.
Nojima, S., Sakuma, M., Nishida, R., and Kuwahara, Y.1999b. A glandular gift in the German cockroach, Blattella germanica(L.) (Dictyoptera: Blattellidae): The courtship feeding of a female on secretions from male tergal glands. J. Insect Behav.12:627–640.
Rice, W. R.1989. Analyzing tables of statistical tests. Evolution43:223–225.
Roth, L. M.1969. The evolution of male tergal glands in Blattaria. Ann. Entomol. Soc. Am.62:176–208.
Roth, L. M. and Dateo, G. P.1966. A sex pheromone produced by males of the cockroach Nauphoeta cinerea. J. Insect Physiol.12:255–265.
Roth, L. M. and Willis, E. R.1952. A study of cockroach behavior. Am. Midl. Nat.47:66–129.
Roth, L.M. and Willis, E. R.1954. The reproduction of cockroaches. Smithson. Misc. Coll. 122:1–49.
Sakuma, M.1998. Probit analysis of preference data. Appl. Entomol. Zool.33:339–347.
Schal, C., Burns, E. L., Jurenka, R. A., and Blomquist, G. J.1990. A new component of the female sex pheromone of Blattella germanica(L.) (Dictyoptera: Blattellidae) and interaction with other pheromone components. J. Chem. Ecol.16:1997–2008.
Sikord, H.1918. Zur Bedeutung der Rückendrüse des Männchens bei der Küchenschabe (Phyllodromia germanica). Z. Angew. Entomol.4:374–375.
Sreng, L.1979. Ultrastructure et chimie de la secretion des glandes tergales du male de Blattella germanica(L.) (Dictyoptera: Blattellidae). Int. J. Insect Morphol. Embryol.8:213–227.
Sreng, L.1993. Cockroach mating behaviors, sex pheromones, and abdominal glands (Dictyoptera: Blaberidae). J. Insect Behav. 6:715–735.
Tsuji, H.1965. Studies on the behaviour pattern of feeding of three species of cockroaches, Blattella germanica(L.), Periplaneta americanaL., and P. fuliginosaS., with special reference to their responses to some constituents of rice bran and some carbohydrates. Jpn. J. Sanit. Zool.16:255–262.
Tsuji, H.1966. Attractive and feeding stimulative effect of some fatty acids and related compounds on three species of cockroaches. Jpn. J. Sanit. Zool.17:89–97.
Wille, J.1920. Biologie und Bek¨ampfung der deutschen Schabe (Phyllodromia germanicaL.). Monogr. Angew. Entomol. Nr. 5, Z. Angew. Entomol.7:1–140.
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Nojima, S., Kugimiya, S., Nishida, R. et al. Oligosaccharide Composition and Pheromonal Activity of Male Tergal Gland Secretions of the German Cockroach, Blattella germanica (L.). J Chem Ecol 28, 1483–1494 (2002). https://doi.org/10.1023/A:1016260905653
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DOI: https://doi.org/10.1023/A:1016260905653