Abstract
Accumulation of two peripheral membrane polypeptides (20 and 28 kDa) in roots of Al-sensitive (cv. Alfor) and Al-resistant (cv. Bavaria) barley cultivars were analysed during Al stress. Both cultivars were subjected to Al concentration ranging from 0 to 150 µM for 24, 48, 72 and 96 h. Accumulation of both polypeptides was determined 24 h after exposure of plants to Al and content of both polypeptides showed only small depedence upon Al concentration and duration of Al treatment. Although, based on root growth test, Bavaria showed significantly greater resistance to Al than Alfor, analysis of 20 and 28 kDa polypeptide pattern has not revealed significant difference between the two cultivars. However, accumulation of 20 and 28 kDa polypeptides in Alfor was selectively induced by Al treatment because different pH of the root media (pH 3.5 to 6.5) or application of other metals (Cu, Co, or Cd) failed to induce these two bands. On the other hand, accumulation of these polypeptides in Bavaria was induced not only by Al, but also by Cd and in a lesser extent by Co treatment.
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Basu, A., Basu, U., Taylor, G.J.: Induction of microsomal membrane proteins in roots of an aluminium-resistant cultivar of Triticum aestivum L. under conditions of aluminium stress.-Plant Physiol. 104: 1007–1013, 1994a.
Basu, U., Basu, A., Taylor, G.J.: Different exudation of polypeptides by roots of aluminum-resistant and aluminum-sensitive cultivars of Triticum aestivum L. in response to aluminum stress.-Plant Physiol. 106: 151–158, 1994b.
Budíková, S., Mistrík, I.: Cultivar characterisation of aluminium tolerance of barley seedlings by root growth, aluminium and callose distribution.-Biologia (Bratislava) 54: 447–451, 1999.
Cacmak, I., Horst, W.J.: Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max).-Physiol. Plant. 83: 463–468, 1991.
Cruz-Ortega, R., Ownby, J.D.: A protein similar to PR (pathogenesis-related) proteins is elicited by metal toxicity in wheat roots.-Physiol. Plant. 89: 211–219, 1993.
Didierjean, L., Frendo, P., Nasser, W., Genot, G., Marivet, J., Burkard, G.: Heavy-metal-responsive genes in maize: identification and comparison of their expression upon various forms of abiotic stress.-Planta 199: 1–8, 1996.
Espino, F.J., González-Jean, M.T., Ibánez, J., Sendino, A.M., Vázquez, A.M.: Aluminum and low pH effects on translatable RNA population from bean calli.-Protoplasma 201: 85–91, 1998.
Ezaki, B., Gardner, C.R., Ezaki, Y., Kondo, H., Matsumoto, H.: Protective roles of two aluminum (Al)-induced genes, HSP150 and SEDI of Saccharomyces cerevisiae, in Al and oxidative stresses.-FEMS Microbiol. Lett. 159: 99–105, 1998.
Ezaki, B., Tsugita, S., Matsumoto, H.: Expression of moderately anionic peroxidase is induced by aluminum treatment in tobacco cells: Possible involvement of peroxidase isozymes in aluminum ion stress.-Physiol. Plant. 96: 21–28, 1996.
Ezaki, B., Yamamoto, Y., Matsumoto, H.: Cloning and sequencing of the cDNA induced by aluminium treatment and Pi starvation in cultured tobacco cells.-Physiol. Plant. 93: 11–18, 1995.
Hamel, F., Breton, C., Houde, M.: Isolation and characterization of wheat aluminum-regulated genes: possible involvement of aluminum as a pathogenesis response elicitor.-Planta 205: 531–538, 1998.
Heukesloven, J., Dernick, R.: Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining.-Electrophoresis 6: 103–112, 1985.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4.-Nature 277: 680–685, 1970.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.
Mistrík, I., Tamás, L., Huttová, J.: Quantitative changes in maize membrane proteins induced by aluminium.-Biol. Plant. 43: 85–91, 2000.
Ownby, J.D., Hruschka, W.R.: Quantitative changes in cytoplasmic and microsomal proteins associated with aluminium toxicity in two cultivars of winter wheat.-Plant Cell Environ. 14: 303–309, 1991.
Richards, K.D., Gardner, R.C.: The effect of aluminium treatment on wheat roots: expression of heat shock, histone and SHH genes.-Plant Sci. 98: 37–45, 1994.
Richards, K.D., Schott, J., Sharma, Y.K., Davis, K.R., Gardner, R.C.: Aluminum induces oxidative stress genes in Arabidopsis thaliana.-Plant Physiol. 116: 409–418, 1998.
Snowden, K.C., Gardner, R.C.: Five genes induced by aluminum in wheat (Triticum aestivum L.) roots.-Plant Physiol. 103: 855–861, 1993.
Snowden, K.C., Richards, K.D., Gardner, R.C.: Aluminium-induced genes. Induction by toxic metals, low calcium, and wounding and pattern of expression in root tips.-Plant Physiol. 107: 341–348, 1995.
Takahashi, Y., Nagata, T.: parB: An auxin-regulated genome encoding glutathione S-transferase.-Proc. nat. Acad. Sci. USA 89: 56–59, 1992.
Tamás, L., Mistrík, I., Huttová, J.: Protein profiles in root of aluminium sensitive and resistant barley cultivars after aluminium treatment.-Biologia (Bratislava) 54: 459–465, 1999.
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Mistík, I., Tamás, L., Huttová, J. et al. Accumulation of Microsomal Polypeptides in Barley Roots during Aluminium Stress. Biologia Plantarum 45, 417–421 (2002). https://doi.org/10.1023/A:1016225902796
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DOI: https://doi.org/10.1023/A:1016225902796