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Effect of dietary fibres on constituents of complex carbohydrates in streptozotocin induced diabetic rat tissues

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Abstract

The effect of dietary fibres on constituents of complex carbohydrates in various tissues of streptozotocin induced diabetic rats is presented by analysing different constituents of complex carbohydrates in presence and absence of dietary fibre. Wheat bran was effective in preventing the decrease (14%) in total sugars in spleen and an increase in total sugars in stomach (33%) during diabetes. Decrease in uronic acid content during diabetes in spleen was prevented to the extent of 25% by the presence of wheat bran in the diet. The other parameters which were affected by the presence of wheat bran in the diet during diabetes are amino sugar (brain and stomach), sulphates (liver) and protein (lungs and stomach). Guar gum was effective in preventing the decrease in total sugar content in spleen by 28% and sulphate content in liver by 14% during diabetes. Variation in protein content in lungs was observed in diabetes. The results indicated beneficial role of dietary fibres like wheat bran and guar gum on complex carbohydrates to varying extents in different tissues.

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References

  1. Trowell H, Burkitt D, Heaten K: In: Dietary Fibre, Fibre Depleted Foods and Diseases. Academic Press, London, 1985

    Google Scholar 

  2. Spiller GA: In: Handbook of Dietary Fibre in Human Nutrition. CRC Press, Florida, 1986

    Google Scholar 

  3. Smith JG, Yokoyama WH, German JB: Butyric acid from the diet: Actions at the level of gene expression. Crit Rev Food Sci Nutr 38: 259–297, 1998

    PubMed  Google Scholar 

  4. Plaami SP: Content of dietary fibre in foods and its physiological effects. Food Rev Intern 13: 29–76, 1997

    Google Scholar 

  5. Bourguin LD, Titgemeyer EC, Fahey GC: Fermentation of various dietary fibre sources by human fecal bacteria. Nutr Res 16: 1119–1131, 1996

    Google Scholar 

  6. Davidson LA, Lupton JR, Jiang YH, Chang WC, Aukema HM, Chapkin RS: Dietary fat and fibre alter rat colonic protein kinase C isozyme expression. J Nutr 125: 49–56, 1995

    PubMed  Google Scholar 

  7. Marsman KE, McBurney MF: Dietary fibre and short chain fatty acids affect cell proliferation and protein synthesis in isolated rat colonocytes. J Nutr 126: 1429–1437, 1996

    PubMed  Google Scholar 

  8. Shah S, Lance P, Smith TJ, Berenson SN, Cohen SA, Horvath PJ, Lan TTY, Baumann H: n-Butyrate reduces the expression of β-galactoside α-2,6–sialyl transferase in HepG2 cells. J Biol Chem 267: 10652–10658, 1992

    PubMed  Google Scholar 

  9. Jocobsson KG, Reinsenfeld J, Lindahl U: Biosynthesis of heparan: Effects of n-butyrate on mast cells. J Biol Chem 260: 12154–12159, 1985

    PubMed  Google Scholar 

  10. Rohrbach DH, Martin GR: Structure of basement membrane in normal and diabetic tissue. Ann NY Acad Sci 401: 203–209, 1982

    PubMed  Google Scholar 

  11. Garlick RL, Bunn HF, Spiro RG: Non-enzymatic glycation of basement membranes from human glomeruli and bovine sources. Effect of diabetes and age. Diabetes 37: 1144–1150, 1988

    Google Scholar 

  12. Shimomura H, Spiro RG: Studies on macro molecular components of human glomerular basement membrane and alterations in diabetes - decreased levels of heparan sulphate proteoglycan and laminin. Diabetes 36: 374–381, 1987

    PubMed  Google Scholar 

  13. Brown DM, Klein DJ, Michael AF, Oegema TR: 35S glycosaminoglycan and 35S glycopeptide metabolism by diabetic glomeruli and aorta. Diabetes 31: 418–425, 1982

    PubMed  Google Scholar 

  14. Nandini CD, Sambaiah K, Salimath PV: Effect of dietary fibre on intestinal and renal disaccharidases in diabetic rats. Nutr Res 20: 1301–1307, 2000

    Google Scholar 

  15. Hatch GM, Cao SG, Angel A: Decreases in cardiac phosphotidyl glycerol in streptozotocin-induced diabetic rats does not affect cardiolipin biosynthesis: Evidence for distinct pools of phosphotidyl glycerol in the heart. Biochem J 306: 759–764, 1995

    PubMed  Google Scholar 

  16. Dahlqvist A: Assay of intestinal disaccharidases. Anal Biochem 22: 99–107, 1968

    PubMed  Google Scholar 

  17. Miller GL: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426–428, 1959

    Google Scholar 

  18. Allalouf D, Ber A, Sharon N: Acid mucopolysaccharides in rat kidneys. Biochim Biophys Acta 83: 278–287, 1964

    PubMed  Google Scholar 

  19. McKelvy JF, Lee YC: Microheterogeneity of the carbohydrate group of Aspergillus oryzae α-amylase. Arch Biochem Biophys 132: 99–110, 1969

    PubMed  Google Scholar 

  20. Bitter T, Muir HM: A modified uronic acid carbozole reaction. Anal Biochem 4: 330–334, 1962

    PubMed  Google Scholar 

  21. Ludoweig J, Benmaman JD: Colorimetric differentiation of hexosamines. Anal Biochem 19: 80–88, 1967

    PubMed  Google Scholar 

  22. Dodgson KS: Determination of inorganic sulphate in studies on enzymic and non-enzymic hydrolyses of carbohydrates and other sulphate esters. Biochem J 78: 312–319, 1961

    PubMed  Google Scholar 

  23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with folin phenol reagent. J Biol Chem 193: 265–272, 1951

    PubMed  Google Scholar 

  24. Snedecor GW, Cochran WG: In: Statistical Methods. Ames Iowa State University Press, USA

  25. Zhang H, Osada K, Sone H, Farukawa Y: Biotin administration improves the glucose tolerance of streptoozotocin induced diabetic Wistar rats. J Nutr Sci Vitaminol 43: 271–280, 1997

    PubMed  Google Scholar 

  26. Berkman J, Rifkin H, Ross G: The serum polysaccharides in diabetic patients with and without degenerative vascular diseases. J Clin Invest 32: 414–421, 1953

    Google Scholar 

  27. Malathy K, Kurup PA: Metabolism of glycosaminoglycans in alloxan diabetic rats. Changes in tissue glycosaminoglycans. Diabetes 21: 1162–1167, 1972

    PubMed  Google Scholar 

  28. Mohanam S, Sudha M: Influence of streptozotocin and alloxan induced diabetes on the metabolism of glycosaminoglycans. Acta Diabetol Lat 21: 203–210, 1984

    PubMed  Google Scholar 

  29. Rathi AN, Vishwanathan A, Shanmugasundaram KR: Studies on protein bound components and glycosaminoglycans in experimental diabetes - effect on Gymnema sylvestre. R Br Ind J Exp Biol 19: 715–721, 1981

    Google Scholar 

  30. Premkumari K, Kurup PA: Effect of feeding cooked tapioca as compared to rice on the metabolism of glycosaminoglycans in rats. Ind J Biochem Biophys 20: 163–166, 1983

    Google Scholar 

  31. Kjellen L, Bielefeld D, Hook M: Reduced sulphation of liver heparan sulphate in experimentally diabetic rats: Diabetes 32: 337–342, 1983

    PubMed  Google Scholar 

  32. Royall D, Wolever TMS, Jeejeebhoy KN: Clinical significance of colonic fermentation. Am J Gastroenterol 85: 1307–1315, 1990

    PubMed  Google Scholar 

  33. Nandini CD, Sambaiah K, Salimath PV: Dietary fibres ameliorate the decreased synthesis of kidney heparan sulphate in diabetic rats. Paper communicated

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  1. Biochemistry and Nutrition Department, Central Food Technological Research Institute, Mysore, 570 013, India

    C.D. Nandini, P.V. Salimath & K. Sambaiah

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  1. C.D. Nandini
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  2. P.V. Salimath
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  3. K. Sambaiah
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Nandini, C., Salimath, P. & Sambaiah, K. Effect of dietary fibres on constituents of complex carbohydrates in streptozotocin induced diabetic rat tissues. Mol Cell Biochem 236, 133–138 (2002). https://doi.org/10.1023/A:1016141406084

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