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Improved long-term culture of hepatocytes in a hydrogel containing Arg-Gly-Asp (RGD)

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Abstract

Freshly harvested primary rat hepatocytes, which had been entrapped in a synthetic extracellular matrix, were examined for differentiated morphology and enhanced liver-specific functions for long-term culture. A copolymer of N-isopropylacrylamide (98 mol % in the feed) and acrylic acid [poly(NiPAAm-co-AAc)], and the adhesion molecule, Arg-Gly-Asp (RGD), incorporated into a hydrogel, were used to entrap hepatocytes. Over 28 days' culture, the hepatocytes in the RGD-incorporated gel maintained higher viability and produced albumin and urea at constant rates, while there was lower cell viability and less albumin secretion by hepatocytes in poly(NiPAAm-co-AAc). Hepatocytes cultured in the gel with RGD incorporated into it constitutes a potentially useful three-dimensional cell system for application in a bio-artificial liver device.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University, 28-2 Yeongun-dong, Jongro-ku, Seoul, 110-749, Korea

    Keun-Hong Park

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  1. Keun-Hong Park
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Park, KH. Improved long-term culture of hepatocytes in a hydrogel containing Arg-Gly-Asp (RGD). Biotechnology Letters 24, 1131–1135 (2002). https://doi.org/10.1023/A:1016138115977

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  • DOI: https://doi.org/10.1023/A:1016138115977

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