Abstract
An efficient liquid culture system for plant regeneration from leaflessstem–root axes of Cryptanthus sinuosus L. B. Smith(Bromeliaceae) was established. High regeneration rates (93%) were achieved inMurashige and Skoog's medium without growth regulators. Whole plants wereobtained in a single-step procedure, resulting in the production of 25.3± 3.6 plants/explant after 6 months of culture. Incubationof plant material at 35 ± 3 °C resulted in an increaseof 60% in the regeneration efficiency compared with tissues incubated at 28± 2 °C. Moreover, after 5–6 sub-cultures in thesame medium, the axes originated bud clusters that could be continuouslymultiplied and gave rise to 19.4 ± 3.2 whole plants per gram of freshmatter. It was estimated that the liquid culture system described is potentiallyable to produce about 4500 plants/explant/year. Rates of 98% acclimatizationwere achieved. The use of plants produced following this method for populationreinforcement and for in vitro preservation programs ofendangered populations is suggested.
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Arrabal, R., Amancio, F., Carneiro, L. et al. Micropropagation of endangered endemic Brazilian bromeliad Cryptanthus sinuosus (L.B. Smith) for in vitro preservation. Biodiversity and Conservation 11, 1081–1089 (2002). https://doi.org/10.1023/A:1015860804695
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DOI: https://doi.org/10.1023/A:1015860804695