Abstract
Major pathways of carbon and sulfur metabolisms were studied in representatives of two clusters of bacteria: Leucothrix thiophila (cluster I, strains 2WS, 4WS, and 6WS) and Leucothrix sp. (cluster II, strains 1WS, 3WS, and 5WS). All strains were capable of chemoorganoheterotrophic growth, as well as of chemolithoheterotrophic growth in the presence of reduced sulfur compounds. The bacteria were found to possess a complete set of the enzymes of the tricarboxylic acid cycle and glyoxylate cycle. The dehydrogenase activity in cells of cluster I strains was an order of magnitude lower than in cluster II strains and in other known heterotrophic bacteria. Cells of bacteria of both clusters exhibited high activity levels of enzymes involved in the energy metabolism of sulfur. The oxidation of sulfur compounds and the operation of the electron-transport chain were shown to be related. Cluster II bacteria more efficiently use organic compounds in their energy metabolism, whereas cluster I bacteria are characterized by more efficient utilization of reduced sulfur compounds. During sulfite oxidation, cluster I bacteria can synthesize ATP both via substrate-level phosphorylation and oxidative phosphorylation, whereas cluster II bacteria synthesize ATP only via the latter process.
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REFERENCES
Brock, T.D., The Genus Leucothrix, The Procaryotes, 1992, pp. 3247–3255.
Dul'tseva, N.M., Dubinina, G.A., and Lysenko, A.N., Isolation of Marine Filamentous Sulfur Bacteria and Description of the New Species Leucothrix thiophila sp. nov., Mikrobiologiya, 1996, vol. 65, no. 1, pp. 89–98.
Dul'tseva, N.M., Taxonomy and Physiology of New Filamentous Sulfur Bacteria, Cand. Sci. (Biol.) Dissertation, Moscow, 1996.
Grabovich, M.Y., Muntyan, M.S., Lebedeva, V.Y., Ustiyan, V.S., and Dubinina, G.A., Lithoheterotrophic Growth and Electron Transfer Chain Components of the Filamentous Gliding Bacterium Leucothrix mucor DSM 2157 during Oxidation of Sulfur Compounds, FEMS Microbiol. Lett., 1999, vol. 178, pp. 155–161.
Grabovich, M.Yu., Dubinina, G.A., Dul'tseva, N.M., and Churikova, V.V., Peculiarities of Carbon Metabolism in the Filamentous Sulfur Bacteria Thiothrix arctophila and Leucothrix thiophila during Chemolitho-and Chemoorganoheterotrophic Growth, Mikrobiologiya, 1996, vol. 65, no. 2, pp. 149–153.
Hyllemon, P.B., Wells, J.S., Krieg, N.R., and Jannasch, H.W., Genus Spirillum: A Taxonomic Study, Int. J. Syst. Bacteriol., 1973, vol. 23, pp. 340–380.
Pfennig, N. and Lippert, K.D., Uber das Vitamin B12-Bedurfnis phototrophes Schwefelbakterien, Arch. Mikrobiol., 1966, vol. 55, pp. 245–256.
Grabovich, M.Yu., Dubinina, G.A., Churikova, V.V., Glushkov, A.F., and Churikov, S.N., Peculiarities of Carbon Metabolism in the Colorless Sulfur Bacterium Macromonas bipunctata, Mikrobiologiya, 1993, vol. 62, no. 3, pp. 421–429.
Petushkova, Yu.P. and Ivanovskii, R.N., Enzymes Involved in the Metabolism of Thiosulfate in Thiocapsa roseopersicina Grown under Various Conditions, Mikrobiologiya, 1976, vol. 45, no. 4, pp. 960–965.
Grabovich, M.Yu., A Study of the Systematics, Physiology, and Peculiarities of Sulfur Metabolism of Colorless Sulfur Bacteria, Cand. Sci. (Biol.) Dissertation, Moscow, 1984.
Reznikov, A.A., Mulikovskaya, E.P., and Sokolov, I.Yu., Metody analiza prirodnykh vod (Methods for the Analysis of Natural Waters), Moscow: Gosgeoltekhizdat, 1963, pp. 97–103.
Kelly, D.P., Chambers, I.A., and Trudinger, P.A., Cyanolysis and Spectrophotometric Estimation of Trithionate in Mixture with Thiosulfate and Tetrathionate, Anal. Chem., 1969, vol. 41, p. 898.
Morris, H.E., Lakombe, R.E., and Lane, W.H., Qualitative Determination of Elemental Sulfur in Aromatic Hydrocarbons, Anal. Chem., 1948, vol. 20, pp. 1037–1039.
Williams, W.J., Handbook of Anion Determination, London: Butteworth, 1979.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, A.J., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.
Kelly, D.P., Physiology and Biochemistry of Unicellular Sulfur Bacteria, Autotrophic Bacteria, Schlegel, H.G. and Bowien, B., Eds., Madison: Science Tech. Publ., 1989, pp. 193–217.
Grabovich, M.Yu. and Mishankova, V.V., Ecologo-Biochemical Peculiarities of the Colorless Filamentous Sulfur Bacterium Leucothrix mucor, II otkrytaya nauchnaya konferentsiya molodykh uchenykh goroda Pushchino. Tez. dokl. (II Open Scientific Conference of the Young Researchers of the Town of Pushchino: Abstracts of Papers), 1997, pp. 165–166.
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Grabovich, M.Y., Dul'tseva, N.M. & Dubinina, G.A. Carbon and Sulfur Metabolism in Representatives of Two Clusters of Bacteria of the Genus Leucothrix: A Comparative Study. Microbiology 71, 255–261 (2002). https://doi.org/10.1023/A:1015838207918
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DOI: https://doi.org/10.1023/A:1015838207918