Skip to main content
SpringerLink
Log in

Preparation and characterization of a bovine serum albumin-catalase conjugate

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A bovine serum albumin-catalase conjugate was prepared using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The purified conjugate had an M r of 380 kDa. The half-life time of the conjugate was 6, 69 and 65 min after heat treatment, urea treatment and trypsin hydrolysis, respectively, whereas that of native catalase was 3, 22, 28 min, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Atalla SL, Toledo-Pereyra LH, MacKenzie GH (1985) Influence of oxygen-derived free radical scavengers on ischemic livers. Transplantation 40: 584–590.

    Google Scholar 

  • Beckman JS, Minor Rl, White CW (1988) Superoxide dismutase and catalase conjugated to polyethylene glycol increased endothelial enzyme activity and oxidant resistance. J. Biol. Chem. 263: 6884–6892.

    Google Scholar 

  • Carlsson J, Drevin H, Axen R (1978) Protein thiolation and reversible protein-protein conjugation. Biochem. J. 173: 723–733.

    Google Scholar 

  • Komatsu T, Hamamatsu K, Takeoka S, Nishide H, Tsuchida E (1998) Human serum albumin-bound synthetic hemes as an oxygen carriers: determination of equilibrium constants for heme binding to host albumin. Art. Cells Blood Subs. Immob. Biotechnol. 26: 519–527.

    Google Scholar 

  • Mao GD, Poznansky MJ (1989) Superoxide dismutase: improving its pharmacological properties by conjugation with human serum albumin. Biomater. Artif. Cells Artif. Organs 7: 229–244.

    Google Scholar 

  • Mehvar R (2000) Modulation of the pharmacokinetics and pharmacodynamics of proteins by polyethylene glycol conjugation. J. Pharm. Pharm. Sci. 3: 125–136.

    Google Scholar 

  • Nguyen WD, Kim DH, Alam HB, Provido HS, Kirkpatrick JR (1999) Polyethylene glycol-superoxide dismutase inhibits lipid peroxidation in hepatic ischemia/reperfusion injury. Crit. Care 3: 127–130.

    Google Scholar 

  • Yokoigawa K, Tanizawa K, Soda K (1989) Chemical modification of urokinase with human serum albumin fragments. Agric. Biol. Chem. 53: 2887–2893.

    Google Scholar 

  • Zimmerman BJ, Granger DN (1992) Reperfusion injury. Surg. Clin. North Amer. 72: 65–83.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, P.O. Box 353, P. R. China

    Tao Hu & Zhiguo Su

Authors
  1. Tao Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhiguo Su
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hu, T., Su, Z. Preparation and characterization of a bovine serum albumin-catalase conjugate. Biotechnology Letters 24, 1027–1030 (2002). https://doi.org/10.1023/A:1015641416286

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1015641416286

Search

Navigation