Skip to main content
SpringerLink
Log in

Liquid-liquid extraction of lectin from Cratylia mollis seeds using reversed micelles

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Extraction of lectin from seeds ofCratylia mollis, camaratu bean, with reversed micelles of 100 mM sodium di(2-ethylhexyl) sulfosuccinate/isooctane was performed firstly with affinity-purified lectin. The best conditions were extraction of the seed extract at pH 5 and back-extraction at pH 10, giving yields of 38% and 100%, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beltrão EIC, Correia MTS, Figueredo-Silva J, Coelho LCBB (1998) Binding evaluation of isoform 1 from Cratylia mollis lectin to human mammary tissues. Appl. Biochem. Biotechnol. 74: 125-134.

    Google Scholar 

  • Carneiro-da-Cunha MG, Melo EP, Cabral JMS, Aires-Barros MR (1996) Liquid-liquid extraction of a recombinant cutinase from fermentation media with AOT reversed micelles. Bioprocess Eng. 15: 151-157.

    Google Scholar 

  • Coelho LCBB, Silva MBR (2000) Simple method to purify milligram quantities of the galactose-specific lectin from the leaves of Bauhinia monandra. Phytochem. Anal. 11: 1-6.

    Google Scholar 

  • Correia MTS, Coelho LCBB (1995) Purification of a glucose/ manose specific lectin, isoform 1, from seeds of Cratylia mollis Mart. (Camaratu bean). Appl. Biochem. Biotech. 55: 261-273.

    Google Scholar 

  • Pires MJL, Prazeres DM, Cabral JMS (1993) Protein assay in reversed micelles solutions. Biotechnol. Tech. 7: 293-294.

    Google Scholar 

  • Sharon N, Lis H (2001) The structural basis for carbohydrate recognition by lectins. In: Wu, ed. The Molecular Immunology of Complex Carbohydrates-2. Taiwan: Kluwer Academic/Plenum Publishers, pp. 1-16.

    Google Scholar 

  • Souza SR, Correia MTS, Pessoa MMA, Kennedy JF, Lima-Filho JL, Coelho LCBB (2001) A novel model to characterize the electric double layer of lectins from Cratylia mollis (Camaratu bean) and Canavalia ensiformis adsorved on metallic surface. Carbohyd. Polym. 46: 191-193.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Bioquímica, Centro de Cîencias Biológicas, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego s/n, 50.670-420, Recife, PE, Brazil

    C.O. Nascimento, L.C.B.B. Coelho, M.T.S. Correia & M.G. Carneiro-da-Cunha

  2. Laboratório de Imunopatologia Keizo Asami – LIKA/UFPE, Recife, PE, Brazil

    C.O. Nascimento & M.G. Carneiro-da-Cunha

Authors
  1. C.O. Nascimento
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L.C.B.B. Coelho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M.T.S. Correia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M.G. Carneiro-da-Cunha
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nascimento, C., Coelho, L., Correia, M. et al. Liquid-liquid extraction of lectin from Cratylia mollis seeds using reversed micelles. Biotechnology Letters 24, 905–907 (2002). https://doi.org/10.1023/A:1015557802326

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1015557802326

Search

Navigation