Abstract
During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect of seeds, and natural and synthetic auxins (4-Cl-IAA, and IAA; 4-Me-IAA, 4-Et-IAA and 4-F-IAA, respectively), and auxin concentration (4-Cl-IAA) on PsGA20ox1 mRNA levels in pea pericarp were investigated over a 24 h treatment period. The ability of the 4-substituted auxins to increase PsGA20ox1 mRNA levels in deseeded pericarp was correlated with their ability to stimulate pericarp growth. The greatest increase in pericarp PsGA20ox1 mRNA levels and growth was observed when deseeded pericarps were treated with the naturally occurring pea auxin, 4-Cl-IAA; however, IAA was not effective. Silver thiosulfate, an ethylene action antagonist, did not reverse IAA's lack of stimulation of PsGA20ox1 over the control treatment. 4-Me-IAA was the second most active auxin in stimulating PsGA20ox1 and was the second most biologically active auxin. Application of the 4-substituted IAA analogs, 4-Et-IAA and 4-F-IAA, to deseeded pericarps resulted in minimal or no increase in PsGA20ox1 transcript levels or pericarp growth. Pericarp PsGA20ox1 mRNA levels increased with increasing 4-Cl-IAA concentration and showed transitory increases at low 4-Cl-IAA treatments (30 to 300 pmol). These results support a unique physiological role for the auxin 4-Cl-IAA in the regulation of GA metabolism by effecting PsGA20ox1 expression during early pea fruit growth.
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References
Ait-Ali, T., Frances, S., Weller, J.L., Reid, J.B., Kendrick, R.E. and Kamiya, Y. 1999. Regulation of gibberellin 20–oxidase and gibberellin 3β-hydroxylase transcript accumulation during de-etiolation of pea seedlings. Plant Physiol. 121: 783–791.
Ait-Ali, T., Swain, S.M., Reid, J.B., Sun, T.-P. and Kamiya, Y. 1997. The LS locus of pea encodes the gibberellin biosynthesis enzyme ent-kaurene synthase A. Plant J. 11: 443–454.
Carrera, E., Jackson, S.D. and Prat, S. 1999. Feedback control and diurnal regulation of gibberellin 20–oxidase transcript levels in potato. Plant Physiol. 119: 765–773.
Coles, J.P., Phillips, A.L., Croker, S.J., Garcia-Lepe, R., Lewis, M.J. and Hedden, P. 1999. Modification of gibberellin production and plant development in Arabidopsis by sense and antisense expression of gibberellin 20–oxidase genes. Plant J. 17: 547–556.
Eeuwens, C.J. and Schwabe, W.W. 1975. Seed and pod wall development in Pisum sativum L. in relation to extracted and applied hormones. J. Exp. Bot. 26: 1–14.
Garcia-Martinez, J.L., Santes, C., Croker, S.J. and Hedden, P. 1991. Identification, quantitation and distribution of gibberellins in fruits of Pisum sativum L. cv. Alaska during pod development. Planta 184: 53–60.
Garcia-Martinez, J.L., Lopez-Diaz, I., Sanchez-Beltran, M.J., Phillips, A.L., Ward, D.A., Gaskin, P. and Hedden, P. 1997. Isolation and transcript analysis of gibberellin 20–oxidase genes in pea and bean in relation to fruit development. Plant Mol. Biol. 33: 1073–1084.
Magnus, V., Ozga, J.A., Reinecke, D.M., Pierson, G.L., Larue, T.A., Cohen, J.D. and Brenner, M.L. 1997. 4–chloroindole-3–acetic acid and indole-3–acetic acid in Pisum sativum. Phytochemistry 46: 675–681.
Martin, D.N., Proebsting, W.M., Parks, T.D., Dougherty, W.G., Lange, T., Lewis, M.J., Gaskin, P. and Hedden, P. 1996. Feedback regulation of gibberellin biosynthesis and gene expression in Pisum sativum L. Planta 200: 159–166.
Marumo, S., Hattori, H., Abe, H. and Munakata, K. 1968. Isolation of 4–chloroindolyl-3–acetic acid from immature seeds of Pisum sativum. Nature 219: 959–960.
Ozga, J.A., Brenner, M.L. and Reinecke, D.M. 1992. Seed effects on gibberellin metabolism in pea pericarp. Plant Physiol. 100: 88–94.
Ozga, J.A. and Reinecke, D.M. 1999. Interaction of 4–chloroindole-3–acetic acid and gibberellins in early pea fruit development. Plant Growth Regul. 27: 33–38.
Phillips, A.L., Ward, D.A., Uknes, S., Appleford, N.E.J., Lange, T., Huttly, A.K., Gaskin, P., Graebe, J.E. and Hedden, P. 1995. Isolation and expression of three gibberellin 20–oxidase cDNA clones from Arabidopsis. Plant Physiol. 108: 1049–1057.
Reinecke, D.M. 1999. 4–Chloroindole-3–acetic acid and plant growth. Plant Growth Regul. 27: 3–13.
Reinecke, D.M. and Ozga, J.A. 1995. IAA and 4–Cl-IAA effect on pea pericarp growth. In: 15th International Conference on Plant Growth Substances (14–18 July 1995, Minneapolis, MN), International Plant Growth Substances Association, p. 447.
Reinecke, D.M., Ozga, J.A. and Magnus, V. 1995. Effect of halogen substitution of indole-3–acetic acid on biological activity in pea fruit. Phytochemistry 40: 1361–1366.
Reinecke, D.M., Ozga, J.A., Ilic, N., Magnus, V. and Kojic-Prodic, B. 1999. Molecular properties of 4–substituted indole-3–acetic acids affecting pea pericarp elongation. Plant Growth Regul. 27: 39–48.
Rodrigo, M.J., Garcia-Martinez, J.L., Santes, C.M., Gaskin, P. and Hedden, P. 1997. The role of gibberellins A1 and A3 in fruit growth of Pisum sativum L. and the identification of gibberellins A4 and A7 in young seeds. Planta 201: 446–455.
Ross, J.J., O'Neill, D.P., Smith, J.J., Kerckhoffs, L.H.J. and Elliott, R.C. 2000. Evidence that auxin promotes gibberellin A1 biosynthesis in pea. Plant J. 21: 547–552.
Santes, C.M. and Garcia-Martinez, J.L. 1995. Effect of the growth retardant 3,5–dioxo-4–butyryl-cyclohexane carboxylic acid ethyl ester, an acylcyclohexanedione compound, on fruit growth and gibberellin content of pollinated and unpollinated ovaries in pea. Plant Physiol. 108: 517–523.
Sponsel, V.M. 1982. Effects of applied gibberellins and naphthylacetic acid on pod development in fruits of Pisum sativum L. cv. Progress No. 9. J. Plant Growth Regul. 1: 147–152.
Sponsel, V.M. 1995. The biosynthesis and metabolism of gibberellins in higher plants. In: P.J. Davies (Ed.) Plant Hormones: Physiology, Biochemistry and Molecular Biology, Kluwer, Dordrecht, Netherlands, pp. 66–97.
Sponsel, V.M., Schmidt, F.W., Porter, S.G., Nakayama, M., Kohlstruk, S. and Estelle, M. 1997. Characterization of new gibberellin-responsive semidwarf mutants of Arabidopsis. Plant Physiol. 115: 1009–1020.
Sponsel, V.M., Khan, S., Colton, S. and Lopez, J. 2000. Gibberellinresponding semidwarf mutants of Arabidopsis: further information, including the possible role of auxins in regulating GA biosynthesis. In: Plant Biology 2000 (15–19 July 2000, San Diego, CA), American Society of Plant Physiologists, p. 727.
Swain, S.M., Reid, J.B. and Kamiya, Y. 1997. Gibberellins are required for embryo growth and seed development in pea. Plant J. 12: 1329–1338.
van Huizen, R., Ozga, J.A., Reinecke, D.M., Twitchin, B. and Mander, L.N. 1995. Seed and 4–cloroindole-3–acetic acid regulation of gibberellin metabolism in pea pericarp. Plant Physiol. 109: 1213–1217.
van Huizen, R., Ozga, J.A. and Reinecke, D.M. 1997. Seed and hormonal regulation of gibberellin 20–oxidase expression in pea pericarp. Plant Physiol. 115: 123–128.
Wu, K., Li, L., Gage, D.A. and Zeevaart, J.A.D. 1996. Molecular cloning and photoperiod-regulated expression of gibberellin 20–oxidase from the long-day plant spinach. Plant Physiol. 110: 547–554.
Xu, Y.-L., Li, L., Wu, K., Peeters, A.J.M., Gage, D.A. and Zeevaart, J.A.D. 1995. The GA5 locus of Arabidopsis thaliana encodes a multifunctional gibberellin 20–oxidase: molecular cloning and functional expression. Proc. Natl. Acad. Sci. USA 92: 6640–6644.
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Ngo, P., Ozga, J.A. & Reinecke, D.M. Specificity of auxin regulation of gibberellin 20-oxidase gene expression in pea pericarp. Plant Mol Biol 49, 439–448 (2002). https://doi.org/10.1023/A:1015522404586
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DOI: https://doi.org/10.1023/A:1015522404586