Abstract
Homeodomain leucine zipper (HDZip) genes encode putative transcription factors that are unique to plants. A function in regulating processes that are specific for plants is postulated, such as responses to environmental cues and developmental signals. This is supported by a growing body of evidence resulting from studies of HDZip genes in a variety of species. In addition to the previously isolated CPHB-1 and -2 genes, this paper reports the isolation of members of five families of Craterostigma plantagineum homeobox leucine zipper genes (CPHB) via a yeast one-hybrid screening approach. Based on the sequence homology and protein interactions the encoded proteins (CPHB-3/4/5/6/7) were classified into HDZip class II and I genes. Homo- and heterodimerization of CPHB proteins within the same structurally related class has been demonstrated and the DNA-binding activity of CPHB proteins to two homeodomain recognition elements (HDE1 and HDE2) has been compared in yeast. All families of CPHB genes were modulated in their expression in response to dehydration in leaves and roots. CPHB-6 and CPHB-7 transcripts accumulated in leaves during early stages of dehydration and decreased after prolonged dehydration. Both transcripts were also induced in ABA-treated callus. CPHB-3/4/5 were down-regulated by dehydration in both leaves and roots. The results support the role of HDZips in regulating programs of gene expression in C. plantagineum that lead to desiccation tolerance.
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Altschul, S.F., Gish, W., Miller, W., Meyers, E.W. and Lipman, D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403–410.
Aso, K., Kato, M., Banks, J. and Hasebe, M. 1999. Characterisation of homeodomain-leucine zipper genes in the fern Ceratopteris richardii and the evolution of the homeodomain-leucine zipper gene family in vascular plants. Mol. Biol. Evol. 16: 544–552.
Baima, S., Nobili, F., Sessa, G., Lucchetti, S., Ruberti, I. and Morelli, G. 1995. The expression of the Athb-8 homeobox gene is restricted to provascular cells in Arabidopsis thaliana. Development 121: 4171–4182.
Bartels, D., Schneider, K., Terstappen, G., Piatkowski, D. and Salamini, F. 1990. Molecular cloning of abscisic acid-modulated genes which are induced during desiccation of the resurrection plant Craterostigma plantagineum. Planta 181: 27–34.
Bray, E.A. 1997. Plant responses to water deficit. Trends Plant Sci. 4: 48–54.
Carabelli, M., Sessa, G., Baima, S., Morelli, G. and Ruberti, I. 1993. The Arabidopsis Athb-2 and-4 genes are strongly induced by far-red-rich light. Plant J 4: 469–479.
Di Cristina, M., Sessa, G., Dolan, L., Linstead, P., Baima, S., Ruberti, I. and Morelli, G. 1996. The Arabidopsis Athb-10 (GLABRA2) is a HD-zip protein required for regulation of root hair development. Plant J. 10: 393–402.
Durfee, T., Becherer, K., Chen, P., Yeh, S., Yang, Y., Kilburn, A., Lee, W. and Elledge, S.J. 1993. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555–569.
Elble, R. 1992. A simple and efficient procedure for transformation of yeast. BioTechniques 13: 18–20.
Frank, W., Phillips, J., Salamini, F. and Bartels, D. 1998. Two dehydration-inducible transcripts from the resurrection plant Craterostigma plantagineum encode interacting homeodomainleucine zipper proteins. Plant J. 15: 413–421.
Gietz, R. and Schiestl, R. 1995. Transforming yeast with DNA. Meth. Mol. Cell Biol. 5: 255–269.
Ingram, J. and Bartels, D. 1996. The molecular basis of dehydration tolerance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 377–403.
Jaglo-Otteeosen, K., Gilmour, S., Zarka, D., Schabenberger, O. and Thomashow, M. 1998. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science 280: 105–106.
Kawahara, R., Komamine, A. and Fukuda, H. 1995. Isolation and characterization of homeobox-containing genes of carrot. Plant Mol. Biol. 27: 155–164.
Lee, Y. and Chun, J. 1998. A new homeodomain-leucine zipper gene from Arabidopsis thaliana induced by water stress and abscisic acid treatment. Plant Mol. Biol. 37: 377–384.
Meijer, A., Scarpella, E., Dijk, E., Qin, L., Taal, A., Rueb, S., Harrington, S., McCouch, S., Schilperoort, R. and Hoge, J. 1997. Transcriptional repression by Oshox1, a novel homeodomain leucine zipper protein from rice. Plant J. 11: 263–276.
Meijer, A., Ouwerkerk, P. and Hoge, J. 1998. Vectors for transcription factor isolation and target gene identification by means of genetic selection in yeast. Yeast 14: 1407–1416.
Meijer, A., Kam, R., d'Erfurth, I., Shen, W. and Hoge, J. 2000. HD-Zip proteins of families I and II from rice: interactions and functional properties. Mol. Gen. Genet. 263: 12–21.
Meissner, R. and Theres, K. 1995. Isolation and characterization of the tomato homeobox gene THOM1. Planta 195: 541–547.
Piatkowski, D., Schneider, K., Salamini, F. and Bartels, D. 1990. Characterisation of five abscisic acid-responsive cDNA clones isolated from the desiccation-tolerant plant Craterostigma plantagineum and their relationship to other water-stress genes. Plant Physiol. 94: 1682–1688.
Ruberti, I., Seea, G., Lucchetti, S. and Morelli, G. 1991. A novel class of plant proteins containing a homeodomain with a closely linked leucine zipper motif. EMBO J. 10: 1787–1791.
Sambrook, J., Fritsch, E. and Maniatis, T. 1998. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Plainview, NY.
Scarpella, E., Rueb, S., Boot, K., Hoge, H. and Meijer, A. 2000. A role for the rice homeobox gene Oshox 1 in provascular cell fate commitment. Development 127: 3655–2669.
Sessa, F., Carabelli, M. and Ruberti, I. 1993. Identification of distinct families of HDZip proteins in Arabidopsis thaliana. In: G. Corruzzi and P. Puigdomènech (Eds.) Plant Molecular Biology, NATO ASI Series vol. H81, Springer-Verlag, Berlin/Heidelberg, pp. 412–426.
Shinozaki, K. and Yamaguchi-Shinozaki, K. 1997. Gene expression and signal transduction in water-stress response. Plant Physiol. 115: 327–334.
Söderman, E., Mattsson, J., Svenson, M., Borkird, C. and Engstrom, P. 1994. Expression patterns of novel genes encoding homeodomain leucine-zipper proteins in Arabidopsis thaliana. Plant Mol. Biol. 26: 145–154.
Söderman, E., Mattsson, J. and Engstrom, P. 1996. The Arabidopsis homeobox gene ATHB-7 is induced by water deficit and by abscisic acid. Plant J. 10: 375–381.
Söderman, E., Hjellstron, N., Fahleson, J. and Engstrom, P. 1999. The HDZip gene ATHB 6 in Arabidopsis is expressed in developing leaves, roots, and carpels and up-regulated by water deficit conditions. Plant Mol. Biol. 40: 1073–1083.
Tornero, P., Conejero, V. and Vera, P. 1996. Phloem-specific expression of a plant homeobox gene during secondary phases of vascular development. Plant J. 9: 639–648.
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Deng, X., Phillips, J., Meijer, A.H. et al. Characterization of five novel dehydration-responsive homeodomain leucine zipper genes from the resurrection plant Craterostigma plantagineum . Plant Mol Biol 49, 601–610 (2002). https://doi.org/10.1023/A:1015501205303
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DOI: https://doi.org/10.1023/A:1015501205303