Abstract
The genetic differentiation and phylogenetic relationships among five species of the Mugilidae family (Mugil cephalus, Chelon labrosus, Liza aurata, Liza ramada, and Liza saliens) were investigated at the mtDNA level, on samples taken from Messolongi lagoon-Greece. RFLP analysis of three PCR-amplified mtDNA gene segments (12s rRNA, 16s rRNA, and CO I) was used. Ten, eight, and nine restriction enzymes were found to have at least one recognition site at 12s rRNA, 16s rRNA, and CO I genes, respectively. Several fragment patterns were revealed to be species-specific, and thus they could be useful in species taxonomy as diagnostic markers, as well as for further evolutionary studies. Seven different haplotypes were detected. The greatest amount of genetic differentiation was observed at the interspecific level, while little variation was revealed at the intraspecific level. The highest values of nucleotide sequence divergence were observed between M. cephalus and all the other species, while the lowest was found between C. labrosus and L. saliens. Dendrograms obtained by the three different methods (UPGMA, Neighbor-Joining, and Dollo parsimony), were found to exhibit in all cases the same topology. According to this, the most distinct species is M. cephalus, while the other species are clustered in two separate groups, the first one containing L. aurata and L. ramada, the other L. saliens and C. labrosus. This last clustering makes the monophyletic origin of the genus Liza questionable.
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REFERENCES
Apostolidis, A. P., Karakousis, Y., and Triantaphyllidis, C. (1996). Genetic differentiation and phylogenetic relationships among Greek Salmo trutta L. (brown trout) populations as revealed by RFLP analysis of PCR amplified mitochondrial DNA segments. Heredity 77:608.
Autem, M., and Bonhomme, F. (1980). Elements de syst´ematique biochimique chez les mugilid´es de M´editerran´ee. Biochem. Syst. Ecol. 8:305.
Avise, J. C. (1991). Ten unorthodox perspectives on evolution prompted by comparative population genetic findings on mitochondrial DNA. Annu. Rev. Genet. 25:45.
Avise, J. C. (1994). Molecular Markers, Natural History and Evolution, Chapman and Hall, New York.
Bernatchez, L., and Osinov, A. (1995). Genetic diversity of trout (genus Salmo) from its most eastern native range based on mitochondrial DNA and nuclear gene variation. Mol. Ecol. 4:285.
Birky, C.W., Fuerst, P., and Maruyama, T. (1989). Organelle gene diversity under migration, mutation, and drift: Equilibrium expectations, approach to equilibrium, effects of heteroplasmic cells, and comparison to nuclear genes. Genetics 121:613.
Birky, C. W., Maruyama, T., and Fuerst, P. (1983). An approach to population and evolution genetic theory for genes in mitochondria and chloroplasts, and some results. Genetics 103:513.
Caldara, F., Bargelloni, L., Ostellari, L., Penzo, E., Colombo, L., and Patarnello, T. (1996). Molecular phylogeny of grey mullets based on mitochondrial DNA sequence analysis: Evidence of differential rate of evolution at the intrafamily level. Mol. Phylogenet. Evol. 6:416.
Cataudella, S., Civitelli, M.V., and Capanna, E. (1974). Chromosome complement of the Mediterranean mullets (Pisces, perciformes). Caryologia 27:93.
Crosetti, D., Nelson, W. S., and Avise, J. C. (1994). Pronounced genetic structure of mitochondrial DNA among populations of the circumglobally distributed grey mullet (Mugil cephalus). J. Fish Biol. 44:47.
Delgado, J. V., Molina, A., Lobillo, J., Alonso, A., and Camacho, M. E. (1992). Morphometrical study on the chromosomes of three species of mullet (Teleostei: Mugilidae). Caryologia 45:263.
Duvernell, D. D., and Aspinwall, N. (1995). Introgression of Luxilus cornutus mtDNA into allopatric populations of Luxilus chrysocephalus (Teleostei: Cyprinidae) in Missouri and Arkansas. Mol. Ecol. 4:173.
Echelle, A. A., and Dowling, T. E. (1992). Mitochondrial DNA variation and evolution of the death valley pupfishes (Cyprinodon, Cyprinodontidae). Evolution 46:193.
Farris, J. S. (1977). Phylogenetic analysis under Dollo's law. Syst. Zool. 26:77.
Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783.
Felsenstein, J. (1993). PHYLIP (Phylogeny Inference Package), Version 3.5C, Department of Genetics, University of Washington, Seattle, WA.
Ferguson, M. M., Danzmann, R. G., and Hutchings, J. A. (1991). Incongruent estimates of population differentiation among brook charr, Salvelinus fontinalis, from Cape Race, Newfoundland, Canada, based upon allozyme and mitochondrial DNA variation. J. Fish Biol. 39:79.
Gonzalez-Villasenor, L. I., and Powers, D. A. (1990). Mitochondrial restriction-site polymorphism's in the teleost Fundulus heteroclitus support secondary integration. Evolution 44:27.
Harrison, I. J., and Howes, G. J. (1991). The pharyngobranchial organ of mugilid fishes; its structure, variability, ontogeny, possible function and taxonomic utility. Bull. Br. Mus. Nat. Hist. (Zool) 57:111.
Jackson, D. P., Hayden, J. D., and Quirke, P. (1991). Extraction of nucleic acid from fresh and archival material. In McPherson, M. J., Quirke, P., and Taylor, G. R. (eds.), PCR, A Practical Approach, IRL Press, Oxford, pp. 29-50.
Kessler, L. G., and Avise, J. C. (1984). Systematic relationships among waterfowl (Anatidae) inferred from restriction endonuclease analysis of mitochondrial DNA. Syst. Zool. 33:370.
Kocher, T. D., Thomas, W. K., Meyer, A., Edwards, S.V., Paabo, S., Villablanca, F. X., and Wilson, A. C. (1989). Dynamics of mitochondrialDNAevolution in animals: Amplification and sequencing with conserved primers. Proc. Natl. Acad. Sci. U.S.A. 86:6196.
Mantel, N. A. (1967). The detection of disease clustering and a generalized regression approach. Cancer Res. 27:209.
Martin, A. P., and Palumbi, S. R. (1993). Body size, metabolic rate, generation time, and the molecular clock. Proc. Natl. Acad. Sci. U.S.A. 90:4087.
McElroy, D., Moran, P., Bermingham, E., and Kornfield, J. (1991). The Restriction Enzyme Analysis Package, version 4.0, University of Maine, Orono, ME.
Meyer, A., Kocher, T. D., Basasilbwa, P., and Wilson, A. C. (1990). Monophyletic origin of Lake Victoria cichlid fishes suggested by mitochondrial DNA sequences. Nature 347:550.
Moran, P., Kornfield, I., and Reinthal, P. N. (1994). Molecular systematics and radiation of the Haplochromine Cichlids (Teleostei: Perciformes) of Lake Malawi. Copeia 2:274.
Moritz, C., Dowling, T. E., and Brown, W. M. (1987). Evolution of animal mitochondrial DNA: Relevance for population biology and systematics. Ann. Rev. Ecol. Syst. 18:269.
Nash, C. E., and Shehadeh, Z. H. (1980). Review of the breeding and propagation techniques for grey mullet, Mugil cephalus. Stud. Rev. 3:87.
Nei, M. (1987). Molecular Evolutionary Genetics, Columbia University Press, New York.
Nelson, J. S. (1994). Fishes of the World, Wiley, New York.
Normark, B. B., McCune, A. R., and Harrison, R. C. (1991). Phylogenetic relationships of neopterygian fish, inferred from mitochondrial DNA sequences. Mol. Biol. Evol. 8:819.
Page, R. D. M. (1996). TREEVIEW: An application to display phylogenetic trees on personal computers. Comp. Appl. Biosci. 12:357.
Palumbi, S., Martin, A., Romano, S., McMillan, W. O., Stice, L., and Grabowski, G. (1991). The Simple Fools Guide to PCR, version II, University of Hawaii, Honolulu.
Papasotiropoulos, V., Klossa-Kilia, E., Kilias, G., and Alahiotis, S. (2001). Genetic divergence and phylogenetic relationships in grey Mullets (Teleostei: Mugilidae) using allozyme data. Biochem. Genet. 39(5/6):155.
Patarnello, T., Bargelloni, L., Caldara, F., and Colombo, L. (1994). Cytochrome b and 16S rRNA sequence variation in the Salmo trutta (Salmonidae, Teleostei) species complex. Mol. Phylogenet. Evol. 3:69.
Patterson, C., Williams, D. M., and Humphries, C. J. (1993). Congruence between molecular and morphological phylogenies. Annu. Rev. Ecol. Syst. 24:153.
Rizzotti, M. (1993). Fish hemoglobins: The family Mugilidae (Perciformes). Trends Comp. Biochem. Physiol. 1:385.
Rohlf, J. (1990). NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, Exeter Software, New York.
Rossi, A. R., Capula, M., Crosetti, D., Campton, D. E., and Sola, L. (1998). Genetic divergence and phylogenetic inferences in five species of Mugilidae (Pisces: Perciformes). Mar. Biol. 131:213.
Saiki, R., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1988). Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 239:487.
Saitou, N., and Nei, M. (1987). The neighbour-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406.
Schultz, L. P. (1946).Arevision of the genera of mullets, fishes of the family Mugilidae with description of three new genera. Proc. U.S.A. Natl. Mus. 96:377.
Senou, H., Randall, J. E., and Okiyama, M. (1996). Chelon persicus, a new species of mullets (Perciformes: Mugilidae) from the persian Gulf. Bull. Kanagawa Prefecture Mus. Nat. Sci. 25:71.
Sneath, P. H. A., and Sokal, R. R. (1973). Numerical Taxonomy-The Principles and Practice of Numerical Classification, Freeman, San Francisco.
Stiassny, M. L. J. (1993). What are grey mullets? Bull. Mar. Sci. 52:197.
Sturmbauer, C., and Meyer, A. (1992). Genetic divergence, speciation and morphological stasis in a lineage of African cichlid fishes. Nature 358:578.
Thomson, J. M. (1981). The taxonomy of grey mullets. In Oren, O. H. (ed.), Aquaculture of Grey Mullets, Cambridge University Press, Cambridge, pp. 1-16.
Thomson, J. M. (1997). The Mugilidae of the world. Mem. Queensland Mus. 41(3):457.
Tortonese, E. (1975). Fauna d' Italia. Osteichthyes (Pessi Ossei). Parte Seconda, Edizioni Calderini, Bologna, pp. 11-26.
Trewavas, E., and Ingham, S. E. (1972). A key to species of Mugilidae in the northeastern Atlantic and Mediterranean, with explanatory notes. J. Zool. Lond. 167:15.
Wilson, A. C., Cann, R. L., Carr, S. M., George, M., Gyllensten, U. B., Helm-Bychowski, K. M., Higuchi, R. G., Palumbi, S. R., Prager, E. M., Sage, R. D., and Stoneking, M. (1985). Mitochondrial DNA and two perspectives on evolutionary genetics. Biol. J. Linn. Soc. 26:376.
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Papasotiropoulos, V., Klossa-Kilia, E., Kilias, G. et al. Genetic Divergence and Phylogenetic Relationships in Grey Mullets (Teleostei: Mugilidae) Based on PCR–RFLP Analysis of mtDNA Segments. Biochem Genet 40, 71–86 (2002). https://doi.org/10.1023/A:1015162304934
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DOI: https://doi.org/10.1023/A:1015162304934