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Genetic analysis of resistance of Bt rice to stripe stem borer (Chilo suppressalis)

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Abstract

Two transgenic Bt rice lines, KMD1 and KMD2, both containing a synthetic cry1Ab gene from Bt, were crossed with conventional rice varieties. The inheritance of resistance to SSB of KMD1 and KMD2was investigated through LSB and field examination of their progenies, e.g. F1, BC1 and F2 populations. In LSBs, 100.0% of newly hatched SSB larvae died on the second day after feeding on leaf tissues of F1 and GUS positive BC1 plants, of which the area of leaf tissues consumed by SSB is also similar to that of transgenic parents. These results imply that the resistance of Bt rice to SSB is dominantly controlled and could be easily exploited in hybrid rice production. Field evaluation showed that segregation ratios for SSB resistance to susceptibility in BC1 populations fit the ratio of 1:1, which was also confirmed by LSBs. However, in F2 populations, the ratio was significantly smaller than 3:1 for resistant to susceptible plants in all 6 indica × japonica (KMD1 and KMD2) crosses, though it fitted 3:1 in all 4 japonica × japonica crosses. The results implied that the resistance of Bt rice to SSB was controlled by a dominant gene which was present in a homozygous condition in both KMD1 and KMD2, but the inheritance could be affected by other factors. Assays for Cry1Ab protein showed that, in most crosses, the content of Cry1Ab is significantly higher in leaves of GUS positive F1, BC1 and F2 plants than that in transgenic Bt parent plants, which accounts for the high resistance observed in these plants to SSB.

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Authors and Affiliations

  1. Institute of Nuclear Agricultural Sciences, Zhejiang University, Hua Jia Chi Campus, Hangzhou, 310029, China

    Zhonghua Wang, Qingyao Shu, Hairui Cui, Dianxing Wu & Yingwu Xia

  2. Institute of Entomology, Department of Plant Protection, Zhejiang University, Hua Jia Chi Campus, Hangzhou, 310029, China

    Gongyin Ye

  3. Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, KIN 6N5, Canada

    Illimar Altosaar

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  1. Zhonghua Wang
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  2. Qingyao Shu
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  3. Gongyin Ye
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  4. Hairui Cui
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  5. Dianxing Wu
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  6. Illimar Altosaar
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  7. Yingwu Xia
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Wang, Z., Shu, Q., Ye, G. et al. Genetic analysis of resistance of Bt rice to stripe stem borer (Chilo suppressalis). Euphytica 123, 379–386 (2002). https://doi.org/10.1023/A:1015096628777

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