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Effect of Synthetic β-Endorphin-Like Peptide Immunorphin on Human T Lymphocytes

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Abstract

β-Endorphin and the synthetic β-endorphin-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (referred to as immunorphin), corresponding to the sequence 364-373 of the CH3 domain of human immunoglobulin G heavy chain, were shown to stimulate concanavalin A-induced proliferation of T lymphocytes from the blood of healthy donors. [Met5]Enkephalin and the antagonist of opioid receptors naloxone examined in parallel were inactive. The stimulating effect of β-endorphin and immunorphin on T lymphocyte proliferation is not inhibited by naloxone. Studies on receptor binding of 125I-labeled immunorphin to T lymphocytes revealed that it binds with high affinity to naloxone-insensitive receptors (K d = 7.0 ± 0.3 nM)). Unlabeled immunorphin completely inhibits 125I-labeled β-endorphin specific binding to naloxone insensitive receptors on T lymphocytes (K i = 0.6 ± 0.1 nM)). Thus, β-endorphin and immunorphin interact with common naloxone insensitive receptors on T lymphocytes.

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Authors and Affiliations

  1. Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow Region, 142290, Russia

    E. V. Navolotskaya, N. V. Malkova, T. A. Zargarova, T. N. Lepikhova, S. B. Krasnova & V. M. Lipkin

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  1. E. V. Navolotskaya
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  2. N. V. Malkova
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  3. T. A. Zargarova
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  4. T. N. Lepikhova
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  5. S. B. Krasnova
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  6. V. M. Lipkin
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Correspondence to E. V. Navolotskaya.

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Navolotskaya, E.V., Malkova, N.V., Zargarova, T.A. et al. Effect of Synthetic β-Endorphin-Like Peptide Immunorphin on Human T Lymphocytes. Biochemistry (Moscow) 67, 357–363 (2002). https://doi.org/10.1023/A:1014844718799

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