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Collagenase production in an Antarctic strain of Arthrobotrys tortor Jarowaja

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Abstract

This paper describes the results of a comparative screening between the nematophagous Antarctic fungus Arthrobotrys tortor and other species of that genus for the production of extracellular collagenases. The nematode species used in this study was Caenorhabditis elegans, feeding on Escherichia coli cultures. Determination of collagenase activity was made using insoluble collagen from bovine Achilles tendon and determining the amount of solubilized hydroxyproline produced. The results show that the total amount of collagenase produced by the Antarctic strain of A. tortor was about threefold higher than that observed for the other species. In the Antarctic strain, collagenase was shown to be a constitutive enzyme.

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Authors and Affiliations

  1. Dipartimento di Ecologia del Territorio e degli Ambienti Terrestri Sezione di Micologia, Universitá di Pavia, Italy, via S. Epifanio 14, 27100

    Solveig Tosi & Ilaria Tosi

  2. Dipartimento di Biochimica, Universitá di Pavia, Italy, via Taramelli 3/B

    Laura Annovazzi, Paolo Iadarola & Giuseppe Caretta

Authors
  1. Solveig Tosi
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  2. Laura Annovazzi
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  3. Ilaria Tosi
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  4. Paolo Iadarola
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  5. Giuseppe Caretta
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Tosi, S., Annovazzi, L., Tosi, I. et al. Collagenase production in an Antarctic strain of Arthrobotrys tortor Jarowaja. Mycopathologia 153, 157–162 (2002). https://doi.org/10.1023/A:1014511105803

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