Abstract
We examined IgA and IgM responses in parotid saliva from human immunodeficiency virus-1 (HIV-1)-infected individuals. Compared to the uninfected controls, levels of salivary secretory IgA2 were significantly increased in HIV-1-infected subjects, with higher levels in those who displayed oral manifestations of HIV-1 infection. Assessed by enzyme immunoassay, about two thirds of the HIV-1-infected individuals tested positive for salivary HIV-1-specific IgA antibodies but not for salivary IgM. No clear correlations between the amount of HIV-1-specific IgA and CD4 counts or plasma viral loads were found. The proportions of HIV-1-specific IgA did not correlate with the levels of total IgA. Determined by Western blot, IgA1 accounted for the majority of anti-HIV-1 IgA antibodies in saliva. Comparisons between the specificities of plasma and salivary IgA directed to HIV-1 proteins revealed the absence of salivary anti-gp41 IgA antibodies, and lower HIV-1-specific reactivity of IgA and IgM were determined in saliva than in plasma.
Similar content being viewed by others
REFERENCES
Mestecky J, Moro I, Underdown BJ: Mucosal Immunoglobulins. In Mucosal Immunology, 2nd Ed, PL Ogra, J Mestecky, ME Lamm, W Strober, J Bienenstock, JR McGhee (eds). New York, Academic Press, 1999, pp 133–152
Mestecky J, Lue C, Russell MW: Selective transport of IgA: Cellular and molecular aspects. Gastroenterol Clin North Am 20:441–471, 1991
McGhee JR, Mestecky J: In defense of surfaces: Development of novel vaccines for IgA responses protective at portals of entry for microbial pathogens. Infect Dis Clin N Am 4:315–341, 1990
Nathanson N, Mathieson BJ: Biological considerations in development of a human immunodeficiency virus vaccine. J Infect Dis 182:579–589, 2000
Kozlowski PA, Jackson S: Serum IgA subclasses and molecular forms in HIV infection: Selective increases in monomer and apparent restriction of the antibody response to IgA1 antibodies mainly directed at env glycoproteins. AIDS Res Hum Retroviruses 8:1773–1780, 1992
Kozlowski PA, Chen D, Eldridge JH, Jackson S: Contrasting IgA and IgG neutralization capacities and responses to HIV type 1 gp120 V3 loop in HIV-infected individuals. AIDS Res Hum Retroviruses 10:813–822, 1994
Cartry O, Moja P, Quesnel A, Pozzetto B, Lucht FR, Genin C: Quantification of IgA and IgG and specificities of antibodies to viral proteins in parotid saliva at different stages of HIV-1 infection. Clin Exp Immunol 109:47–53, 1997
Matsuda S, Oka S, Honda M, Takebe Y, Takemori T: Characteristics of IgA antibodies against HIV-1 in sera and saliva from HIV-seropositive individuals in different clinical stages. Scand J Immunol 38:428–434, 1993
Poignard P, Klasse PJ, Sattentau QJ: Antibody neutralization of HIV-1. Immunol Today 17:239–246, 1996
Sattentau QJ: Neutralization of HIV-1 by antibody. Curr Opin Immunol 8:540–545, 1996
Sattentau QJ, Moore JP: Human immunodeficiency virus type 1 neutralization is determined by epitope exposure on the gp120 oligomer. J Exp Med 182:185–196, 1995
Burnett P, VanCott T, Polonis V, Redfield R, Birx D: Serum IgA-mediated neutralization of HIV type 1. J Immunol 152: 4642–4648, 1994
Black KP, Cummins JE Jr., Jackson S: Serum IgA from HIVseropositive individuals mediates antibody dependent cellular cytotoxicity against HIV-infected cells. Clin Immunol Immunopathol 81:182–190, 1996
Moja P, Tranchat C, Tchou I, Pozzetto B, Lucht F, Desgranges C, Genin C: Neutralization of human immunodeficiency virus type 1 (HIV-1) mediated by parotid IgA of HIV-1-infected patients. J Infect Dis 181:1607–1613, 2000
Raux M, Finkielsztejn L, Salmon-Céron D, Bouchez H, Excler JL, Dulioust E, Grouin JM, Sicard D, Blondeau C: Comparison of the distribution of IgG and IgA antibodies in serum and various mucosal fluids of HIV type 1-infected subjects. AIDS Res Hum Retroviruses 15:1365–1376, 1999
Lü XS, Delfraissy JF, Grangeot-Keros L, Rannou MT, Pillot J: Rapid and constant detection of HIV antibody response in saliva of HIV-infected patients; selective distribution of anti-HIV activity in the IgG isotype. Res Virol 145:369–377, 1994
Archibald DW, Barr CE, Torosian JP, McLane MF, Essex M: Secretory IgA antibodies to human immunodeficiency virus in the parotid saliva of patients with AIDS and AIDS-related complex. J Infect Dis 155:793–796, 1987
Renom G, Bouquety JC, Lanckriet C, Georges AJ, Siopathis MR, Martin PMV: HIV-specific IgA antibodies in tears of children with AIDS or at risk of AIDS. Res Virol 141:355–363, 1990
Bélec L, Bouquety JC, Georges AJ, Siopathis MR, Martin PMV: Antibodies to human immunodeficiency virus in the breast milk of healthy seropositive women. Pediatrics 85:1022–1026, 1990
Van de Perre P, Simonon A, Hitimana DG, Dabis F, Msellati P, Mukamabano B, Butera JB, Van Goethem C, Karita E, Lepage P: Infective and anti-infective properties of breast milk from HIV-1-infected women. Lancet 341:914–918, 1993
Janoff EN, Jackson S, Wahl SM, Thomas K, Peterman JH, Smith PD: Intestinal mucosal immunoglobulins during human immunodeficiency virus type 1 infection. J Infect Dis 170:299–307, 1994
Bélec L, Georges AJ, Steenman G, Martin PMV: Antibodies to human immunodeficiency virus in vaginal secretions of heterosexual women. J Infect Dis 160:385–391, 1989
O'Shea S, Cordery M, Barrett WY, Richman DD, Bradbeer C, Banatvala JE: HIV excretion patterns and specific antibody response in body fluids. J Med Virol31:291–296, 1990
Lü XS, Bélec L, Pillot J: Anti-gp160 IgG and IgA antibodies associated with a large increase in total IgG in cervicovaginal secretions from human immunodeficiency virus type 1-infected women. J Infect Dis 167:1189–1192, 1993
Archibald DW, Witt DJ, Craven DE, Vogt MW, Hirsch MS, Essex M: Antibodies to human immunodeficiency virus in cervical secretions from women at risk for AIDS. J Infect Dis 156: 240–241, 1987
Bélec L, Georges AJ, Steenman G, Martin PMV: Antibodies to human immunodeficiency virus in the semen of heterosexual men. J Infect Dis 159:324–327, 1989
Wolff H, Mayer K, Seage G, Politch J, Horsburgh CR, Anderson D: A comparison of HIV-1 antibody classes, titers, and specificities in paired semen and blood samples from HIV-1 seropositive men. J Acquir Immune Defic Syndr 5:65–69, 1992
Mohamed OA, Ashley R, Goldstein A, McElrath J, Dalessio J, Corey L: Detection of rectal antibodies to HIV-1 by a sensitive chemiluminescent western blot immunodetection method. J Acquir Immune Defic Syndr 7:375–380, 1994
Jackson S: Secretory and serum IgA are inversely altered in AIDS patients. In Advances in Mucosal Immunology, TT MacDonald, SJ Challacombe, PW Bland, CR Stokes, RV Heatley, AM Mowat (eds). London, Kluwer Academic, 1990, pp 665–668
Müller F, Frøland SS, Hvatum M, Radl J, Brandtzaeg P: Both IgA subclasses are reduced in parotid saliva from patients with AIDS. Clin Exp Immunol 83:203–209, 1991
Sweet SP, Rahman D, Challacombe SJ: IgA subclasses in HIV disease: Dichotomy between raised levels in serum and decreased secretion rates in saliva. Immunology 86:556–559, 1995
Grimoud AM, Arnaud C, Dellamonica P, Lodter JP: Salivary defence factor concentrations in relation to oral and general parameters in HIV positive patients. Eur J Oral Sci 106:979–985, 1998
Coogan MM, Sweet SP, Challacombe SJ: Immunoglobulin A (IgA), IgA1, and IgA2 antibodies to Candida albicans in whole and parotid saliva in human immunodeficiency virus infection and AIDS. Infect Immun 62:892–896, 1994
Mergener K, Enzensberger W, Rübsamen-Waigmann H, Briesen HV, Doerr HW: Immunoglobulin class-and subclass-specific HIV antibody detection in serum and CSF specimens by ELISA and western blot. Infection 15:317–322, 1987
Khalife J, Guy B, Capron M, Kieny MP, Ameisen JC, Montagnier L, Lecocq JP, Capron A: Isotype restriction of the antibody response to human immunodeficiency virus. AIDS Res Hum Retroviruses 4:3–9, 1988
Müller F, Müller KH: Detection of anti-HIV-1 immunoglobulin M antibodies in patients with serologically proved HIV-1 infection. Infection 16:115–118, 1988
Lashley KS: Reflex secretion of human parotid gland. J Exp Psychol 1:461–493, 1916
Kabir S: Jacalin: A jackfruit (Artocarpus heterophyllus) seedderived lectin of versatile applications in immunobiological research. J Immunol Methods 212:193–211, 1998
Wu X, Jackson S: Plasma and salivary IgG subclasses in HIV type 1 infection: Evidence of both transudation and local synthesis of IgG in parotid saliva. AIDS Res Hum Retroviruses 16:1423–1431,2000
Klasse PJ, Pipkorn R, Blomberg J: Presence of antibodies to a putatively immunosuppressive part of human immunodeficiency virus (HIV) envelope glycoprotein gp41 is strongly associated with health among HIV-positive subjects.Proc Natl Acad Sci USA 85:5225–5229, 1988
Lal RB, Heiba IM, Dhawan RR, Smith ES, Perine PL: IgG subclass responses to human immunodeficiency virus-1 antigens: Lack of IgG2 response to gp41 correlates with clinical manifestation of disease. Clin Immunol Immunopathol 58:267–277, 1991
Levy JA: Pathogenesis of human immunodeficiency virus infection. Microbiol Rev 57:183–289, 1993
Lane HC, Masur H, Edgar LC, Whalen G, Rook AH, Fauci AS: Abnormalities of B-cell activation and immunoregulation in patients with the acquired immunodeficiency syndrome. N Engl J Med 309:453–458, 1983
Reimer CB, Black CM, Holman RC, Wells TW, Ramirez RM, Sa-Ferreira JA, Nicholson JK, McDougal JS: Hypergammaglobulinemia associated with human immunodeficiency virus infection. Monogr Allergy 23:83–96, 1988
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wu, X., Jackson, S. Plasma and Salivary IgA Subclasses and IgM in HIV-1-Infected Individuals. J Clin Immunol 22, 106–115 (2002). https://doi.org/10.1023/A:1014435920321
Issue Date:
DOI: https://doi.org/10.1023/A:1014435920321