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Changing the `Denkstil' – A Case Study in the History of Molecular Genetics

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Abstract

The importance of experimental systems for the analysis of historical processes in science has grown in recent years. In this paper interactions between experimental systems and modes of interpretation are discussed. The discovery of the `base pairing principle' by Erwin Chargaff serves as an example to show how established laboratory routines and their development interact with the underlying theoretical framework and the `Denkstil' (style of thought) of a collective of researchers as it can be reconstructed from their publications. Unexpected evidence frequently produced by the experimental system initially escapes a satisfactory explanation and tends to initiate a process of self-conviction accompanied by a change of concepts and explanatory models so that they finally fit into interpretations conforming with the `Denkstil'.

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References

  • Abir-Am, P.: 1980, ‘From Biochemistry to Molecular Biology: DNA and the Acculturated Journey of the Critic of Science Erwin Chargaff’, History and Philosophy of Life Sciences 2, 3-60.

    Google Scholar 

  • Avery, O.T., MacLeod, C.M. & McCarthy, M.: 1944, ‘Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types’, J. Exp. Med 79, 137-158.

    Google Scholar 

  • Bendich, A., Pahl, H.B. & Beiser, S.M: 1956, ‘Chromatographic Fractionation of Desoxyribonucleic Acid with Special Emphasis on the Transforming Factor’, Cold Spring Harbour Symp. Quant. Biol. 21, 31-48.

    Google Scholar 

  • Chargaff, E.: 1944, ‘Lipoproteins’, Adv. Protein Chemistry 1, 1-24.

    Google Scholar 

  • Chargaff, E.: 1947, ‘On the Nucleoproteins and Nucleic Acids of Microorganisms’S, Cold Spring Harbor Symp. Quant. Biol. 12, 28-34.

    Google Scholar 

  • Chargaff, E.: 1950, ‘Chemical Specifity of Nucleic Acids and Mechanism of their Enzymatic Degradation’, Experientia 6(6), 201-240.

    Google Scholar 

  • Chargaff, E.: 1951a, ‘Structure and Function of Nucleic Acid as Cell Constituent’, Federation Proceedings 10, 654-659.

    Google Scholar 

  • Chargaff, E.: 1951b, ‘Some Recent Studies on the Composition and Structure of Nucleic Acids’, J. Cell. Comp. Physiol. 38 (Suppl.), 41-59.

    Google Scholar 

  • Chargaff, E.: 1955a, ‘The Isolation and Composition of Desoxypentose Nucleic Acid and of the Corresponding Nucleoproteins’, in E. Chargaff & J.N. Davidson (eds), The Nucleic Acids Chemistry and Biology, Vol. 1, Chap. 10, pp. 307-371.

  • Chargaff, E.: 1955b, ‘Desoxypentose Nucleoproteins and their Prosthetic Groups’, Symp. Soc. Exp. Biol. 9, 32-48.

    Google Scholar 

  • Chargaff, E.: 1955c, ‘On the Chemistry and Function of Nucleoproteins and Nucleic Acids’, Istituto Lombardo (Rend. Sc.) 89, 101-115.

    Google Scholar 

  • Chargaff, E.: 1978, Heraclitean Fire: Sketches from a Life before Nature, The Rockefeller University Press, New York.

    Google Scholar 

  • Chargaff, E. & Vischer, E.: 1948, ‘Nucleoproteins, Nucleic Acids, and Related Substances’, Ann. Rev. Biochem. 17, 201-226.

    Google Scholar 

  • Chargaff, E. & Zamenhof, S.: 1948a, ‘The Isolation of Highly Polymerized Desoxypentose Nucleic Acid from Yeast Cells’, J. Biol. Chem. 173, 327-335.

    Google Scholar 

  • Chargaff, E. & Zamenhof, S.: 1948b, ‘Desoxypentosenuclease in Yeast and Specific Nature of its Cellular Regulation’, Science 108, 628-629.

    Google Scholar 

  • Chargaff, E. & Saidel, H.F.: 1949, ‘On Nucleoproteins of Avian Tubercle Bacilli’, J. Biol. Chem. 177, 417-428.

    Google Scholar 

  • Chargaff, E. & Lipshitz, R.: 1953, ‘Composition of Mammalian Desoxyribonucleic Acid’, Amer. Chem. Soc. J. 75, 3658-3661.

    Google Scholar 

  • Chargaff, E., Vischer, E., Doniger, R. Green, C. & Misani, F.: 1949a, ‘The Composition of the Desoxypentose Nucleic Acid of Thymus and Spleen’, J. Biol. Chem. 177, 405-416.

    Google Scholar 

  • Chargaff, E., Magasanik, B., Doniger, R. & Vischer, E.: 1949b, ‘The Nucleotide Composition of Ribonucleic Acid’, Amer. Chem. Soc. J. 71, 1513-1514.

    Google Scholar 

  • Chargaff, E., Magasanik, B., Vischer, E., Green, C., Doniger, R. & Elson, D.: 1950a, ‘Nucleotide Composition of Pentose Nucleic Acids from Yeast and Mammalian Tissues’, J. Biol. Chem. 186, 51-67.

    Google Scholar 

  • Chargaff, E., Zamenhof, S. & Green, C.: 1950b, ‘Composition of Human Desoxypentose Nucleic Acid’, Nature 165, 756-757.

    Google Scholar 

  • Chargaff, E., Zamenhof, S., Brawerman, G. & Kerin, L.: 1950c, ‘Bacterial Desoxypentose Nucleic Acid of Unusual Composition’, Amer. Chem. Soc. J. 72, 3825.

    Google Scholar 

  • Chargaff, E., Lipshitz, R., Green, C. & Hodes, M.E.: 1951, ‘The Composition of the Desoxypentose Nucleic Acids of Salmon Sperm’, J. Biol. Chem. 192, 223-230.

    Google Scholar 

  • Chargaff, E., Lipshitz, R. & Green, C.: 1952, ‘Composition of the Desoxypentose Nucleic Acids of Four Genera of Sea-Urchin’, J. Biol. Chem. 195, 155-160.

    Google Scholar 

  • Chargaff, E., Crampton, C. F. & Lipshitz, R.: 1953, ‘Separation of Calf Thymus Desoxypentose Nucleic Acid into Fractions of Different Composition’, Nature 172, 289-292.

    Google Scholar 

  • Crampton, C.F., Lipshitz, R. & Chargaff, E.: 1954, ‘Studies on Nucleoproteins–II. Fractionation of Deoxyribonucleic Acids through Fractional Dissociation of their Complexes with Basic Proteins’, J. Biol. Chem. 211, 125-142.

    Google Scholar 

  • Elson, D. & Chargaff, E.: 1955, ‘Evidence of Common Regularities in the Composition of Pentose Nucleic Acids’, Biochim. Biophys. Acta 17, 367-376.

    Google Scholar 

  • Elson, D., Trent, L.W. & Chargaff, E.: 1955, ‘The Nucleotide Composition of Pentose Nucleic Acids in Different Cellular Fractions’, Biochim. Biophys. Acta 17, 362-366.

    Google Scholar 

  • Fleck, L.: 1980, Entstehung und Entwicklung einer wissenschaftlichen Tatsache. Suhrkamp Verlag, Frankfurt a.M. (Engl. edition: Fleck, L.: 1979, The Genesis and Development of a Scientific Fact, T.J. Trenn & R.K. Merton (eds), Chicago).

    Google Scholar 

  • Gandelman, B., Zamenhof, S. & Chargaff, E.: 1952, ‘The Desoxypentose Nucleic Acid of Three Strains of Escherichia Coli’, Biochim. Biophys. Acta 9, 399-401.

    Google Scholar 

  • Gulland, J.M., Baker, G.R. & Jordan, D.O.: 1945, ‘The Chemistry of Nucleic Acids and Nucleoproteins’, Ann. Rev. Biochem. 14, 175-206.

    Google Scholar 

  • Gulland, J.M., Jordan, D.O. & Taylor, H.F.W.: 1947, J. Chem. Soc. 1131-1141.

  • Judson, H.F.: 1980, ‘Reflections on the Historiography of Molecular Biology’, Minerva 18, 369-421.

    Google Scholar 

  • Magasanik, B. & Chargaff, E.: 1951, ‘Studies on the Structure of Ribonucleic Acids’, Biochim. Biophys. Acta 7, 396-412.

    Google Scholar 

  • Magasanik, B., Vischer, E., Doniger, R., Elson, D. & Chargaff, E.: 1950, ‘The Separation and Estimation of Ribonucleotides in Minute Quantities’, J. Biol. Chem. 186, 37-50.

    Google Scholar 

  • Rheinberger, H.-J.: 1997, Toward a History of Epistemic Things–Synthesizing Proteins in the Test Tube, Stanford University Press, Stanford California.

    Google Scholar 

  • Rheinberger, H.-J.: 1998, ‘Kurze Geschichte der Molekularbiologie’, in I. Jahn (ed.), Geschichte der Biologie, 3. Aufl. G. Fischer, Jena, pp. 642-663.

    Google Scholar 

  • Tamm, C. & Chargaff, E.: 1953, ‘Physical and Chemical Properties of the Apurinic Acid of Calf Thymus’, J. Biol. Chem. 203, 689-694.

    Google Scholar 

  • Tamm, C., Hodes, M.E. & Chargaff, E.: 1952, ‘The Formation of Apurinic Acid from Desoxyribo-nucleic Acid of Calf Thymus’, J. Biol. Chem. 195, 49-63.

    Google Scholar 

  • Tamm, C., Shapiro, H.S., Lipshitz, R. & Chargaff, E.: 1953, ‘Distribution Density of Nucleotides within a Desoxyribonucleic Acid Chain’, J. Biol. Chem. 203, 673-688.

    Google Scholar 

  • Vischer, E. & Chargaff, E.: 1947, ‘The Separation and Characterisation of Purines in Minute Amounts of Nucleic Acid Hydrolysates’, J. Biol. Chem. 168, 781-782.

    Google Scholar 

  • Vischer, E. & Chargaff, E.: 1948a, ‘The Separation and Quantitative Estimation of Purines and Pyrimidines in Minute Amounts’, J. Biol. Chem. 176, 703-714.

    Google Scholar 

  • Vischer, E. & Chargaff, E.: 1948b, ‘The Composition of the Pentose Nucleic Acid of Yeast and Pancreas’, J. Biol. Chem. 176, 715-734.

    Google Scholar 

  • Vischer, E., Zamenhof, S. & Chargaff, E.: 1949a, ‘Microbial Nucleic Acids: Desoxypentose Nucleic Acids of Avian Tubercle Bacilli and Yeast’, J. Biol. Chem. 177, 429-438.

    Google Scholar 

  • Vischer, E., Magasanik, B. & Chargaff, E.: 1949b, ‘Chromatographic Separation of Ribonucleotides and Its Application to Study Ribonucleic Acid Structure’, Federation Proceedings 8, 263-264.

    Google Scholar 

  • Watson, J.D. & Crick, F.H.C.: 1953, ‘Molecular Structure of Nucleic Acids’, Nature 171(4356), 737-738.

    Google Scholar 

  • Zamenhof, S. & Chargaff, E.: 1949, ‘Evidence of the Existence of a Core in Desoxypentose Nucleic Acid’, J. Biol. Chem. 178, 531-532.

    Google Scholar 

  • Zamenhof, S. & Chargaff, E.: 1950a, ‘Studies on the Diversity and the Native State of Desoxypentose Nucleic Acid’, J. Biol. Chem. 186, 207-219.

    Google Scholar 

  • Zamenhof, S. & Chargaff, E.: 1950b, ‘Dissymmetry in Nucleotide Sequence of Desoxypentose Nucleic Acid’, J. Biol. Chem. 187, 1-14.

    Google Scholar 

  • Zamenhof, S., Shettles, L.B. & Chargaff, E.: 1950, ‘Isolation of Desoxypentose Nucleic Acid from Human Sperm’, Nature 165, 756.

    Google Scholar 

  • Zamenhof, S., Brawerman, G. & Chargaff, E.: 1952, ‘On the Desoxypentose Nucleic Acid from Several Microorganisms’, Biochim. Biophys. Acta 9, 402-405.

    Google Scholar 

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Ohly, K.P. Changing the `Denkstil' – A Case Study in the History of Molecular Genetics. Science & Education 11, 155–167 (2002). https://doi.org/10.1023/A:1014400903653

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