Abstract
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme that was reported to contain 1 mol of covalently bound FAD and 1 mol of non-covalently-bound FAD. This work describes the result of reinvestigation of the cofactors in this enzyme. The enzyme was found to contain 1 mol of non-covalently-bound NAD+, 1 mol of non-covalently-bound FAD, and 1 mol of covalent FMN. The covalent FMN was identified by the mass and amino acid sequence analyses of the flavin peptide.
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Mukouyama, E.B., Ohsawa, H. & Suzuki, H. Cofactors in Sarcosine Oxidase from Corynebacterium sp. U-96. J Protein Chem 21, 59–64 (2002). https://doi.org/10.1023/A:1014135216860
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DOI: https://doi.org/10.1023/A:1014135216860