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Integral caa3-Cytochrome c Oxidase from Thermus thermophilus: Purification and Crystallization

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Protein Self-Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2039))

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Abstract

Cytochrome c oxidase is a respiratory enzyme catalyzing the energy-conserving reduction of molecular oxygen to water—a fundamental biological process of cell respiration. The first crystal structures of the type A cytochrome c oxidases, bovine heart and Paracoccus denitrificans cytochrome c oxidases, were published in 1995 and contributed immensely to the understanding of the enzyme’s mechanism of action. The senior author’s research focus was directed toward understanding the structure and function of the type B cytochrome c oxidases, ba3-oxidase and type A2 caa3-oxidase, both from the extreme thermophilic bacterium Thermus thermophilus. While the ba3-oxidase structure was published in 2000 and functional characterization is well-documented in the literature, we recently successfully solved the structure of the caa3-nature made enzyme-substrate complex. This chapter is dedicated to the purification and crystallization process of caa3-cytochrome c oxidase.

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Authors and Affiliations

  1. Department of Chemical Sciences, Bernal Institute, University of Limerick, Limerick, Ireland

    Orla Slattery, Sabri Cherrak & Tewfik Soulimane

  2. Department of Biopharmaceutical and Medical Science, Galway-Mayo Institute of Technology, Galway, Ireland

    Orla Slattery

  3. Department of Biology, University Abou-Bekr Belkaid, Tlemcen, Algeria

    Sabri Cherrak

Authors
  1. Orla Slattery
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  2. Sabri Cherrak
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  3. Tewfik Soulimane
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Corresponding author

Correspondence to Orla Slattery .

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Editors and Affiliations

  1. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland

    Jennifer J. McManus

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Slattery, O., Cherrak, S., Soulimane, T. (2019). Integral caa3-Cytochrome c Oxidase from Thermus thermophilus: Purification and Crystallization. In: McManus, J. (eds) Protein Self-Assembly. Methods in Molecular Biology, vol 2039. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9678-0_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9678-0_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9677-3

  • Online ISBN: 978-1-4939-9678-0

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