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Microsatellite primed-PCR to select molecular markers for Tuber species

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Abstract

The direct microsatellite-primed PCR and the RAMPO techniques were applied to detect inter-specific polymorphisms in Tuber species and to select species specific fragments. A T. borchii marker was identified and specific primers were selected.

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References

  • Amicucci A, Rossi I, Potenza L, Agostini D, Stocchi V (1997) Use of sequence characterised amplified region and RAPD markers in the identification of the white truffle Tuber magnatum Pico. Biotechnol. Tech. 11: 149-154.

    Google Scholar 

  • Amicucci A, Zambonelli A, Giomaro G, Potenza L, Stocchi V (1998) Identification of ectomycorrhizal fungi of the genus Tuber by species-specific ITS primers. Mol. Ecol. 7: 273-277.

    Google Scholar 

  • Bachman K (1993) Molecular markers in plant ecology. New Phytol. 126: 403-418.

    Google Scholar 

  • Bertini L, Potenza L, Zambonelli A, Amicucci A, Stocchi V (1998) Restriction fragment length polymorphism species-specific patterns in the identification of white truffles. FEMS Microbiol. Lett. 164: 397-401.

    Google Scholar 

  • Brown TA (1991) In: Hames BD, Rickwood D, eds. Molecular Biology LabFax. Oxford: Information Press, Ltd.

    Google Scholar 

  • Don RH, Cox PT, Wainright BI, Baker K, Mattick JS (1991) Touch-down PCR to circumvent spurious priming during gene amplification. Nucl. Acid Res. 79: 4008.

    Google Scholar 

  • Longato S, Bonfante P (1997) Molecular identification of mycorrhizal fungi by direct amplification of microsatellite regions. Mycol. Res. 101: 425-432.

    Google Scholar 

  • Mello A, Garnero L, Bonfante P (1999) Specific PCR-primers as a reliable tool for the detection of white truffles in mycorrhizal roots. New Phytol. 141: 511-516.

    Google Scholar 

  • Pegler DN, Spooner BM, Young TWK (1993) British Truffles. A Revision of British Hypogeous Fungi. Kew: Royal Botanic Garden.

    Google Scholar 

  • Potenza L, Amicucci A, Rossi I, Palma F, De Bellis R, Cardoni P, Stocchi V (1994) Identification of Tuber magnatum Pico DNA markers by RAPD analysis. Biotechnol. Tech. 8: 93-98.

    Google Scholar 

  • Richardson T, Cato S, Ramser J, Kahl G, Weising K (1995) Hybridization of microsatellites to RAPD: a new source of polymorphic markers. Nucl. Acids Res. 23: 3798-3799.

    Google Scholar 

  • Rubini A, Paolocci F, Granetti B Arcioni S (1998) Single step molecular characterization of morphologically similar black truffle species. FEMS Microbiol. Lett. 164: 7-12.

    Google Scholar 

  • Weising K, Atkinson RG, Gardner RC (1995) Genomic fingerprinting by microsatellite-primed PCR: a critical evaluation. PCR Meth. Appl. 4: 249-255.

    Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingley SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 22: 6531-6535.

    Google Scholar 

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Amicucci, A., Guidi, C., Potenza, L. et al. Microsatellite primed-PCR to select molecular markers for Tuber species. Biotechnology Letters 24, 263–267 (2002). https://doi.org/10.1023/A:1014080527613

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