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Simulation of the Mechanism of Liquid Stationary Phase Retention in a Rotating Coiled Column: Hydrophilic Liquid Systems

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Abstract

A mathematical model was developed which describes the behavior of various liquid systems in a rotating coiled column used in countercurrent chromatography. The conditions of retaining the liquid stationary phase were described in detail for hydrophilic (slowly settling) systems. Various forces that act on liquids in the column were considered. Equations were derived for the steady-state retention process. Mathematical expressions were obtained which make it possible to estimate the stationary-phase volume retained in a column with consideration for the physicochemical properties of the liquid system in use, the speed of column rotation, and the main design parameters of the planet centrifuge. This volume directly affects the column capacity and separation efficiency, and it is crucial for choosing liquid systems and experimental conditions.

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Authors and Affiliations

  1. Russian Academy of Sciences, Vernadsky Institute of Geochemistry and Analytical Chemistry, ul. Kosygina 19, Moscow, 119991, Russia

    P. S. Fedotov, V. A. Kronrod, T. A. Maryutina & B. Ya. Spivakov

Authors
  1. P. S. Fedotov
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  2. V. A. Kronrod
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  3. T. A. Maryutina
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  4. B. Ya. Spivakov
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Fedotov, P.S., Kronrod, V.A., Maryutina, T.A. et al. Simulation of the Mechanism of Liquid Stationary Phase Retention in a Rotating Coiled Column: Hydrophilic Liquid Systems. Journal of Analytical Chemistry 57, 145–149 (2002). https://doi.org/10.1023/A:1014003621155

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  • DOI: https://doi.org/10.1023/A:1014003621155

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