Abstract
A new polymer-stabilized nematic liquid crystal has been characterized for the measurement of biomolecular residual dipolar couplings. Filamentous Pf1 phage were embedded in a polyacrylamide matrix that fixes the orientation of the particles. The alignment was characterized by the quadrupolar splitting of the 2H NMR water signal and by the measurement of 1H-15N residual dipolar couplings (RDC) in the archeal translation elongation factor 1β. Protein dissolved in the polymer-stabilized medium orients quantitatively as in media without polyacrylamide. We show that the quadrupolar splitting and RDCs are zero in media in which the Pf1 phage particles are aligned at the magic angle. This allows measurement of J and dipolar couplings in a single sample.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Al-Hashimi, H.M., Valafar, H., Terrell, M., Zartler, E.R. and Eidsness, M. K. (2000) J. Magn. Reson., 143, 402–406.
Barrientos, L.G., Dolan, C. and Gronenborn, A.M. (2000) J. Biomol. NMR, 16, 329–337.
Bradley, D.E. (1973) Can. J. Microbiol., 19, 623–631.
Brünger, A.T., Adams, P.D., Clore, G.M., DeLano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.S., Kuszewski, J., Nilges, M., Pannu, N.S., Read, R.J., Rice, L.M., Simonson, T. and Warren, G.L. (1998) Acta. Crystallogr. D Biol. Crystallogr., 54, 905–921.
Cavagnero, S., Dyson, H.J. and Wright, P.E. (1999) J. Biomol. NMR, 13, 387–391.
Clore, G.M., Starich, M.R. and Gronenborn, A.M. (1998) J. Am. Chem. Soc., 120, 10571–10572.
Fleming, K., Gray, D., Prasannan, S. and Matthews, S. (2000) J. Am. Chem. Soc., 122, 5224–5225.
Hansen, M.R., Mueller, L. and Pardi, A. (1998a) Nat. Struct. Biol., 5, 1065–1074.
Hansen, M.R., Rance, M. and Pardi, A. (1998b) J. Am. Chem. Soc., 120, 11210–11211.
Koenig, B.W., Hu, J.-S., Ottiger, M., Bose, S., Hendler, R.W. and Bax, A. (1999) J. Am. Chem. Soc., 121, 1385–1386.
Kozlov, G., Ekiel, I., Beglova, N., Yee, A., Dharamsi, A., Engel, A., Siddiqui, N., Nong, A. and Gehring, K. (2000) J. Biomol. NMR, 17, 187–194.
Kraulis, P.J. (1991) J. Appl. Crystallogr., 24, 946–950.
Losonczi, J.A. and Prestegard, J.H. (1998) J. Biomol. NMR, 12, 447–451.
Ottiger, M. and Bax, A. (1998) J. Biomol. NMR, 12, 361–372.
Ottiger, M. and Bax, A. (1999) J. Biomol. NMR, 13, 187–191.
Prosser, R.S., Losonczi, J.A. and Shiyanovskaya, I. V. (1998) J. Am. Chem. Soc., 120, 11010–11011.
Sass, H.-J., Musco, G., Stahl, S.J., Wingfield, P.T. and Grzesiek, S. (2000) J. Biomol. NMR, 18, 303–309.
Sass, J., Cordier, F., Hoffmann, A., Rogowski, M., Cousin, A., Omichinski, J.G., Löwen, H. and Grzesiek, S. (1999) J. Am. Chem. Soc., 121, 2047–2055.
Saupe, A. and Englert, G. (1963) Phys. Rev. Lett., 11, 462–465.
Tian, F., Losonczi, J.A., Fischer, M.W. and Prestegard, J.H. (1999) J. Biomol. NMR, 15, 145–150.
Tjandra, N. and Bax, A. (1997) Science, 278, 1111–1114.
Tolman, J.R., Al-Hashimi, H.M., Kay, L.E. and Prestegard, J.H. (2001) J. Am. Chem. Soc., 123, 1416–1424.
Tycko, R., Blanco, F.J. and Ishii, Y. (2000) J. Am. Chem. Soc., 122, 9340–9341.
Zandomeneghi, G., Tomaselli, M., van Beek, J.D. and Meier, B.H. (2001) J. Am. Chem. Soc., 123, 910–913.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Trempe, JF., Morin, F.G., Xia, Z. et al. Characterization of polyacrylamide-stabilized Pf1 phage liquid crystals for protein NMR spectroscopy. J Biomol NMR 22, 83–87 (2002). https://doi.org/10.1023/A:1013832422428
Issue Date:
DOI: https://doi.org/10.1023/A:1013832422428