Skip to main content
SpringerLink
Log in

Al behavior in tobacco cells by NAA and staining method

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

In the study of aluminum toxicity for plant, it is important to analyzethe behavior of Al in cells. We present how Al is taken up by tobacco cellsthrough lumogallion staining method developed by our laboratory. The fluorescenceintensity from the cell was increased rapidly between 4 and 8 hours of 1 mMAl treatment. To calibrate the fluorescence intensity from Al-lumogallioncomplex, Al amount in the cell was determined by NAA. When the same samplewas analyzed by ICP-AES, Al amounts in all the samples were 13% lower thanthose measured by NAA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L. V. Kochian, Annu. Rev. Plant Physiol. Mol. Biol., 46 (1995) 237.

    Article  CAS  Google Scholar 

  2. Z. Rengel, New Physiol., 134 (1996) 389.

    Article  CAS  Google Scholar 

  3. G. J. Taylor, Metal Ions Biol. Systems, 24 (1988) 123.

    CAS  Google Scholar 

  4. T. Kataoka,M. Mori,T. M. Nakanishi,S. Matsumoto,A. Uchiumi, J. Plant Res., 110 (1997) 305.

    Article  CAS  Google Scholar 

  5. T. Kataoka,H. Iikura,T. M. Nakanishi, Plant Nut. for Sustainable Food Production and Environment, 1997, p. 427.

  6. Y. Yamamoto,S. Rikiishi,Y. C. Chang,K. Ono,M. Kasai,H. Matsumoto, Plant Cell Physiol., 35 (1994) 575.

    Article  CAS  Google Scholar 

  7. T. Nagata,K. Okada,I. Takabe, Plant Cell Rep., 1 (1982) 250.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, Japan, 113-8657

    H. Iikura, K. Tanoi & T. M. Nakanishi

Authors
  1. H. Iikura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Tanoi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. M. Nakanishi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Iikura.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Iikura, H., Tanoi, K. & Nakanishi, T.M. Al behavior in tobacco cells by NAA and staining method. Journal of Radioanalytical and Nuclear Chemistry 249, 499–502 (2001). https://doi.org/10.1023/A:1013265308371

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1013265308371

Keywords

Search

Navigation