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Addition of nickel to Murashige and Skoog medium in plant tissue culture activates urease and may reduce metabolic stress

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Abstract

Nickel has been identified as an essential element for plant growth. The lack of nickel and the inclusion of cobalt leads to absence of urease activity in plants grown on MS medium in tissue culture. To avoid leaf damage and metabolic stress, nickel should be included in tissue culture growth media.

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Authors and Affiliations

  1. Unit of Plant Biochemistry, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

    Claus-Peter Witte, Sarah A. Tiller, Mark A. Taylor & Howard V. Davies

Authors
  1. Claus-Peter Witte
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  2. Sarah A. Tiller
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  3. Mark A. Taylor
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  4. Howard V. Davies
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Correspondence to Claus-Peter Witte.

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Witte, CP., Tiller, S.A., Taylor, M.A. et al. Addition of nickel to Murashige and Skoog medium in plant tissue culture activates urease and may reduce metabolic stress. Plant Cell, Tissue and Organ Culture 68, 103–104 (2002). https://doi.org/10.1023/A:1012966218478

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  • DOI: https://doi.org/10.1023/A:1012966218478

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