Abstract
The concentrations of platelet-activating factor(PAF), leukotriene-B4 (LTB4), andtumour necrosis factor-α (TNF-α) inhomogenate supernatants of gastric mucosal biopsyspecimens and in gastric juice from Helicobacter pylori-positive (N =21) and-negative children (N = 14) were investigated inorder to determine whether these lipid mediators and thecytokine are involved in the inflammatory reaction of H. pylori-associated gastritis. PAF andLTB4 concentrations were measured afterhigh-performance liquid chromatography (HPLC)purification by specific radioimmunoassay, andTNF-α concentrations were determined by using an enzyme-linkedimmunosorbent assay. The concentrations of PAF, LTB, andTNF-α measured in gastric juice and biopsyhomogenate supernatants of children with H. pyloripositive gastritis were found to be statisticallyelevated and in positive correlation with each other.This study suggested that increased local mucosalproduction of potent proinflammatory agents such as PAF, LTB4, and TNF-α may beimplicated in the pathogenesis of H. pylori-associatedgastritis in childhood.
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Huseyinov, A., Kutukculer, N., Aydogdu, S. et al. Increased Gastric Production of Platelet-Activating Factor, Leukotriene-B4, and Tumor Necrosis Factor-α in Children with Helicobacter pylori Infection. Dig Dis Sci 44, 675–679 (1999). https://doi.org/10.1023/A:1012941020790
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DOI: https://doi.org/10.1023/A:1012941020790