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Helicobacter pylori Peptidyl Prolyl Isomerase Expression Is Associated with the Severity of Gastritis

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Abstract

Purpose

Helicobacter pylori secretory peptidyl prolyl isomerase, HP0175, is progressively identified as a pro-inflammatory and pro-carcinogenic protein, which serves to link H. pylori infection to its more severe clinical outcomes. Here, we have analyzed host HP0175-specific antibody responses in relation to the severity of gastritis.

Methods

The HP0175 gene fragment was PCR-amplified, cloned, expressed and purified by Ni-NTA affinity chromatography. Serum antigen-specific antibody responses of non-ulcer dyspeptic patients (N = 176) against recombinant HP0175 were detected by western blotting. The infection status of these subjects was determined by rapid urease test, culture, histology, and serology. The grade of inflammation and stage of atrophy were scored blindly according to the OLGA staging system.

Results

The recombinant HP0175 (rHP0175) was expressed as a ~35 kDa protein and its identity was confirmed by western blotting using anti-6X His tag antibody and pooled H. pylori-positive sera. Serum IgG antibodies against rHP0175 segregated our patients into two similar-sized groups of sero-positives (90/176, 51.1 %) and sero-negatives (86/176, 48.9 %). The former presented with higher grades of gastric inflammation (OR = 4.4, 95 % CI = 1.9–9.9, P = 0.001) and stages of gastric atrophy (OR = 18.3, 95 %CI = 1.4-246.6, P = 0.028).

Conclusion

Our findings lend further support to the pro-inflammatory nature of H. pylori peptidyl prolyl isomerase (HP0175) and recommends this antigen as a non-invasive serum biomarker of the severity of H. pylori-associated gastritis.

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Acknowledgments

This study was generously supported by a technical assistance grant (IRN-072), which was co-funded by the Islamic Development Bank, Saudi Arabia, and Pasteur Institute of Iran.

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Authors and Affiliations

  1. HPGC Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran

    Akbar Oghalaie, Samaneh Saberi, Maryam Esmaeili, Fatemeh Ebrahimzadeh & Marjan Mohammadi

  2. Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran

    Farzaneh Barkhordari

  3. Fungal Biotechnology Lab, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran

    Abdolreza Ghamarian & Vahid Khalaj

  4. Department of Gastroenterology, Amiralam Hospital, Tehran University of Medical Sciences, Tehran, 1145765111, Iran

    Mohammad Tashakoripoor & Mahmoud Eshagh Hosseini

  5. Cancer Institute, Tehran University of Medical Sciences, Tehran, 141979733141, Iran

    Afshin Abdirad

Authors
  1. Akbar Oghalaie
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  2. Samaneh Saberi
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  3. Maryam Esmaeili
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  4. Fatemeh Ebrahimzadeh
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  6. Abdolreza Ghamarian
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  9. Mahmoud Eshagh Hosseini
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  10. Vahid Khalaj
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  11. Marjan Mohammadi
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Corresponding author

Correspondence to Marjan Mohammadi.

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The authors declare no conflict of interest.

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Akbar Oghalaie and Samaneh Saberi share first authorship.

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Oghalaie, A., Saberi, S., Esmaeili, M. et al. Helicobacter pylori Peptidyl Prolyl Isomerase Expression Is Associated with the Severity of Gastritis. J Gastrointest Canc 47, 375–380 (2016). https://doi.org/10.1007/s12029-016-9849-x

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  • DOI: https://doi.org/10.1007/s12029-016-9849-x

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