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Concomitant accumulation of intracellular free calcium and arachidonic acid in the ischemic-reperfused rat heart

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Abstract

This study was designed to elucidate the relationship between enhanced cytoplasmic calcium levels (Ca2+ i) and membrane phospholipid degradation, a key step in the loss of cellular integrity during cardiac ischemia/reperfusion-induced damage. Isolated rat hearts were subjected to 15 min ischemia followed by 30 min reperfusion. Ca2+ i was estimated by the Indo-1 fluorescence ratio technique. Degradation of membrane phospholipids as indicated by the increase of tissue arachidonic acid content was assessed in tissue samples taken from the myocardium at various points of the ischemia/reperfusion period. The hemodynamic parameters showed almost complete recovery during reperfusion. Fluorescence ratio increased significantly during ischemia, but showed a considerable heart-to-heart variation during reperfusion. Based upon the type of change of fluorescence ratio during reperfusion, the hearts were allotted to two separate subgroups. Normalization of fluorescence ratio was associated with low post-ischemic arachidonic acid levels. In contrast, elevated fluorescence ratio coincided with enhanced arachidonic acid levels. This observation is suggestive for a relationship between the Ca2+-related fluorescence ratio and arachidonic acid accumulation probably due to a calcium-mediated stimulation of phospholipase A2.

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Authors and Affiliations

  1. Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary

    Tamás Ivanics, Zsuzsa Miklós, Kornélia Ikrényi, András Tóth & László Li geti

  2. Department of Vascular Pharmacology, Gedeon Richter Ltd., Budapest, Hungary

    László Dézsi

  3. Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands

    Theo H.M. Roemen & Ger J. Van der Vusse

Authors
  1. Tamás Ivanics
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  2. Zsuzsa Miklós
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  3. László Dézsi
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  4. Kornélia Ikrényi
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  5. András Tóth
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  6. Theo H.M. Roemen
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  7. Ger J. Van der Vusse
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  8. László Li geti
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Correspondence to Ger J. Van der Vusse.

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Ivanics, T., Miklós, Z., Dézsi, L. et al. Concomitant accumulation of intracellular free calcium and arachidonic acid in the ischemic-reperfused rat heart. Mol Cell Biochem 226, 119–128 (2001). https://doi.org/10.1023/A:1012739722150

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  • DOI: https://doi.org/10.1023/A:1012739722150

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