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Visualisation of metal deposition in biofilm reactors by three-dimensional magnetic resonance imaging (MRI)

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Abstract

Three-dimensional magnetic resonance imaging (MRI) was used to visualise polyurethane foam-immobilised Citrobacter after challenging with La3+ and/or Cu2+ in citrate buffer supplemented with glycerol 2-phosphate. Extensive phosphatase-mediated bioaccumulation of LaPO4 was observed but no evidence for deposition of Cu3(PO4)2 was obtained by X-ray diffraction and proton-induced X-ray emission analyses. Image analysis showed that La3+/Cu2+ is a good model system to study the function of this biofilm reactor non-invasively by MRI.

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Nott, K.P., Paterson-Beedle, M., Macaskie, L.E. et al. Visualisation of metal deposition in biofilm reactors by three-dimensional magnetic resonance imaging (MRI). Biotechnology Letters 23, 1749–1757 (2001). https://doi.org/10.1023/A:1012492216390

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