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Analytical Methodologies for Biofilm Research pp 243–266Cite as

Nuclear Magnetic Resonance (NMR) and Surface Plasmon Resonance (SPR) Imaging as an Advanced Tool for Examining Biofilm Matrix (Structure, Composition, and Dynamics)

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Abstract

Despite a surge in biofilm characterization research in recent years, it is still an incompletely understood conundrum, especially due to its highly complex, temporally and spatially variable intrinsic parameters. In fact, the increased persistence and resistance of biofilm is governed by a critical ensemble of chemical, physical and mechanical properties such as extracellular polymeric substances (EPS) composition, nutrient gradient, metabolite content, shear stress, hydrodynamics, mass transport etc. Surface Plasmon Resonance and Nuclear Magnetic Resonance imaging uniquely provide label-free tracking of quantitative, local changes caused by movement, adhesion, flow and removal of bacterial cells and other components. The greatest advantage of them over other super resolution imaging and microscopy techniques is their probe independent, non-invasive mode of working in real time scale based on population level analysis of the biofilms. In this chapter, nuclear magnetic resonance and surface plasmon resonance approaches in biological, biomedical, industrial, environmental research are summarized with detailed insight into the fundamentals and working principles of these techniques, case specific applications, suitability and advantages of the methods. Furthermore, possibilities and limitations are recapitulated and analyzed with respect to open research problems and recent technical and methodical developments. This would help interdisciplinary researchers’ in crafting experimental strategy according to their study objectives, standardizing sample selection, parameter choice, data collection and analysis in biofilm structure, composition and dynamics imaging.

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Authors and Affiliations

  1. Department of Biotechnology, Govt of India, Laboratory of NanoChemistry and Chemical Biology, Regional Centre for Biotechnology, Faridabad, India

    Madhurima Mitra

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  1. Madhurima Mitra
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  1. Department of Biotechnology, University of Engineering & Management, Kolkata, West Bengal, India

    Moupriya Nag

  2. Department of Biotechnology, University of Engineering & Management, Kolkata, West Bengal, India

    Dibyajit Lahiri

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Mitra, M. (2021). Nuclear Magnetic Resonance (NMR) and Surface Plasmon Resonance (SPR) Imaging as an Advanced Tool for Examining Biofilm Matrix (Structure, Composition, and Dynamics). In: Nag, M., Lahiri, D. (eds) Analytical Methodologies for Biofilm Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-1378-8_11

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