Abstract
A method has been developed for preparing recombinant baculovirus that encoded foreign protein (using β-galactosidase ofEscherichia colias an example) directly inBombyx morilarvae (silkworm) and avoids cultivation and the use of a cell line, which are very difficult and require expensive equipment and reagents. This significantly reduces the cost of the recombinant protein without loss of biological properties
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REFERENCES
K. L. Miller, Agricultural Biotechnology: Vector Systems of Molecular Cloning, Agropromizdat, Moscow (1991).
E. V. Uporova,Sh. Sh. Alimukhamedov,A. D. Lebedev,Yu. E. Ionova,A. Sh. Tashpulatov, and Sh. S. Azimova, Khim. Prir. Soedin., 684 (1998).
S. Maeda, in: Invertebrate Cell System Application, J. Mitsuhashi, ed., CRC Press, Boca Raton, V. 1, 1988, p. 167.
A. D. Lebedev,E. V. Uporova, and Sh. S. Azimova, Uzbekistan Pat. No. IDP 04398 (1999); Rasmii Akhborotnoma, No. 4 (2000).
A. V. Mazin,K. D, Kuznedelov,A. S. Kraev, et al., Methods of Molecular Genetics and Gene Engineering [in Russian], Nauka, Sibir. Otd., Novosibirsk (1990).
J. Sambrook,F. F. Fritsch, and T. Maniatis, Molecular Cloning. A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, (1989), pp. 98, 239 and 337.
O. H. Lowry and F. J. Rosenbrough, J. Biol. Chem., 193, 265 (1961).
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Uporova, E.V., Azimova, S.S. Preparation of Recombinant Baculovirus DNA Coding for β-Galactosidase of Escherichia coli and the Expression Product in Bombyx mori Larvae. Chemistry of Natural Compounds 37, 181–184 (2001). https://doi.org/10.1023/A:1012395306785
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DOI: https://doi.org/10.1023/A:1012395306785