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5,7-Dihydroxytryptamine: Regional Brain Concentrations Following Intraventricular Administration to Rats

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Abstract

The concentrations of 5,7-dihydroxytryptamine (5,7-DHT) and serotonin (5-HT) were measured in brainstem, hypothalamus and cerebral cortex 0, 2, 6, 12, and 24 hours following the bilateral, lateral ventricular injection of 5,7-DHT (100 μg/each ventricle) into adult male rats. At 6 hours, 5,7-DHT levels had decreased 99% from 0 hr values in all brain regions. Thereafter, 5,7-DHT levels continued to decline in cortex, but not in hypothalamus or brainstem; at 24 hr, but not 48 hr, 5,7-DHT peaks were still measurable in each brain region examined. Serotonin levels in all three regions also fell markedly by 2-6 hours after 5,7-DHT administration. At 24 hours, hypothalamus and brainstem 5HT levels had declined >70% and cerebral cortex ∼50% below control values. The relevance of these findings to the protective action of monoamine reuptake blockers is discussed.

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REFERENCES

  1. Bjorklund, A., Nobin, A., and Stenevi, U. 1973. Regeneration of central serotonin neurons after axonal degeneration induced by 5,6-dihydroxytryptamine. Brain Res. 50:214–220.

    Google Scholar 

  2. Baumgarten, H. G. and Zimmerman, B. 1994. Neurotoxic phenylalkylamines and indolealkylamines. Pages 225–291, in Herken, H., Hucho, F. (eds.), Selective Neurotoxicity, Springer-Verlag, Berlin.

    Google Scholar 

  3. Towle, A. C., Breese, G. R., Mueller, R. A., Coyle, S., and Lauder, J. M. 1984. Early postnatal administration of 5,7-DHT: Effects on serotonergic neurons and terminals. Brain Res. 310:67–75.

    Google Scholar 

  4. Frankfurt, M. and Azmitia, E. 1983. The effect of intracerebral injections of 5,7-dihydroxytryptamine and 6-hydroxydopamine on the serotonin-immunoreactive cell bodies and fibers in the adult rat hypothalamus. Brain Res. 261:91–99.

    Google Scholar 

  5. Bjorklund, A., Baumgarten, H.-G., and Nobin, A. 1974. Chemical lesioning of central monoamine axons by means of 5,6-dihydroxytryptamine and 5,7-dihydroxytryptamine. Adv. Biochem. Psychopharmacol. 10:13–33.

    Google Scholar 

  6. Fuller, R. W. 1978. Neurochemical effects of serotonin neurotoxins: An introduction. Ann. NY Acad. Sci. 305:178–181.

    Google Scholar 

  7. Bjorklund, A., Baumgarten, H. G., and Rensch, A. 1975. 5,7-dihydroxytryptamine: Improvement of its selectivity for serotonin neurons in the CNS by pretreatment with desipramine. J. Neurochem. 24:833–835.

    Google Scholar 

  8. Breese, G. R. and Cooper, B. R. 1975. Behavioral and biochemical interactions of 5,7-dihydroxytryptamine with various drugs when administered intracisternally to adult and developing rats. Brain Res. 98:517–527.

    Google Scholar 

  9. Baumgarten, H. G., Lachenmayer, L., and Bjorklund, A. 1977. Chemical lesioning of indoleamine pathways. Pages 47–98, in Myers, R. D. (eds.), Methods in Psychobiology: Advanced laboratory techniques in neuropsychology and neurobiology, Academic Press, New York.

    Google Scholar 

  10. Creveling, C. R. and Rotman, A. 1978. Mechanism of action of dihydroxytryptamines. Ann. NY Acad. Sci. 305:57–73.

    Google Scholar 

  11. Paxinos, G. and Watson, C. 1998. The Rat Brain in Stereotaxic Coordinates. Academic Press, San Diego, California.

    Google Scholar 

  12. O'Connell, M. T., Portas, C. M., Sarna, G. S., and Curzon, G. 1991. Effect of p-chlorophenylalanine on release of 5-hydroxytryptamine from the rat frontal cortex in vivo. Br. J. Pharmacol. 102:831–836.

    Google Scholar 

  13. Lowry, O. H., Rosebrough, M., Farr, A., and Randall, R. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  14. Baumgarten, H. G., Klemm, H. P., and Lachenmayer, L. 1978. Mode and mechanism of action of neurotoxic indoleamines: A review and a progress report. Ann. NY Acad. Sci. 305:3–24.

    Google Scholar 

  15. Klemm, H. P., Baumgarten, H. G., and Schlossberger, H. G. 1980. Polarographic measurements of spontaneous and mitochondria-promoted oxidation of 5,6-and 5,7-dihydroxytryptamine. J. Neurochem. 35:1400–1408.

    Google Scholar 

  16. Creveling, C. R., Lundstrom, J., McNeal, E. T., Tice, L., and Daly, J. W. 1975. Dihydroxytryptamines: Effects on noradrenergic function in mouse heart in vivo. Mol. Pharmacol. 11:211–222.

    Google Scholar 

  17. Daniel, W. and Melzacka, M. 1992. A comparative study on desipramine pharmacokinetics in the rat brain after administration of desipramine or imipramine. J. Pharm. Pharmacol. 44:429–432.

    Google Scholar 

  18. Argenti, D. and D'Mello, A. P. 1994. The pharmacodynamics of desipramine and desmethyldesipramine in rats. J. Pharmacol. Exp. Therap. 270:512–519.

    Google Scholar 

  19. Caccia, S., Cappi, M., Fracasso, C., and Garattini, S. 1990. Influence of dose and route of administration on the kinetics of fluoxetine and its metabolite norfluoxetine in the rat. Psychopharmacol. 100:509–514.

    Google Scholar 

  20. Bjorklund, A., Horn, A. S., Baumgarten, H. G., Nobin, A., and Schlossberger, H. G. 1975. Neurotoxicity of hydroxylated tryptamines: Structure-activity relationships. 2. In vitro studies on monoamine uptake inhibition and uptake impairment. Acta Physiol. Scand. 429(Suppl):29–60.

    Google Scholar 

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Authors and Affiliations

  1. Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA

    SuJean Choi & Vaishali Patil

  2. Pharmacology and Neuroscience, University of Pittsburgh School of Medicine, Pittsburgh, PA

    John D. Fernstrom

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  1. SuJean Choi
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  2. Vaishali Patil
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  3. John D. Fernstrom
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Correspondence to SuJean Choi.

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Choi, S., Patil, V. & Fernstrom, J.D. 5,7-Dihydroxytryptamine: Regional Brain Concentrations Following Intraventricular Administration to Rats. Neurochem Res 26, 1145–1149 (2001). https://doi.org/10.1023/A:1012374923662

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