Skip to main content
SpringerLink
Log in

Superresolution Chromatography

  • Published:
Instruments and Experimental Techniques Aims and scope Submit manuscript

Abstract

A method for improving the resolution of the chromatographic analysis based on deriving the point-spread function of a chromatographic column, i.e., a chromatogram of an individual compound, is described. A system of two functions—a chromatogram of the substance analyzed and a point-spread function of the chromatographic column—in combination with noise statistics enables the application of a RECOVERY signal-reconstruction software package in order to obtain a superresolution chromatogram. The superresolution means a better resolution than that determined by the width of the point-spread function. The proposed method is tested with a bovine serum albumin chromatography with the use of gel filtration. The resolution obtained exceeds that reached with the use of high-performance liquid chromatography (with a lower cost of the instrument system by a factor of 15–20).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Sakodynskii, K.I., Brazhnikov, V.V., Volkov, S.A., et al., Analiticheskaya khromatografiya (Analytical Chromatography), Moscow: Khimiya, 1993, pp. 16–21.

    Google Scholar 

  2. Friefelder, D., Physical Biochemistry, San Francisco: Freeman, 1976. Translated under the title Fizicheskaya biokhimiya, Moscow: Mir, 1980, pp. 200–201.

    Google Scholar 

  3. Kosarev, E.L., Prib. Tekh. Eksp., 1989, no. 4, p. 84.

  4. Kosarev, E.L., Radiotekh. Elektron. (Moscow), 1990, vol. 35, no. 1, p. 68.

    Google Scholar 

  5. Gel'fgat, V.I., Kosarev, E.L., and Podolyak, E.R., Prib. Tekh. Eksp., 1991, no. 5, p. 86; Available from VINITI, 1991, Moscow, no. 2635-V91.

  6. Gelfgat, V.I., Kosarev, E.L., and Podolyak, E.R., Comput. Phys. Commun., 1993, vol. 74, no. 3, p. 335.

    Google Scholar 

  7. Linnik, Yu.V., Metod naimen'shikh kvadratov i osnovy teorii obrabotki nablyudenii (Least Squares Method and Principles of Data Processing), Moscow: Fizmatgiz, 1962, ch. 3, sect. 3.

    Google Scholar 

  8. Kosarev, E.L., Peskov, V.D., and Podolyak, E.R., Zh. Tekh. Fiz., 1983, vol. 53, no. 6, p. 1101.

    Google Scholar 

  9. Korotkikh, V.L., Kosarev, E.L., Ormont, A.B., and Korotkikh, A.V., Prib. Tekh. Eksp., 1994, no. 6, p. 88.

  10. Kaminskii, A.S., Kosarev, E.L., and Lavrov, E.V., Prib. Tekh. Eksp., 1997, no. 3, p. 57.

  11. Kolganova, E.A., Kosarev, E.L., and Ososkov, G.A., Nucl. Instrum. Methods Phys. Res. A, 2000, vol. 443, p. 464.

    Google Scholar 

  12. Kirillov, V.I., Prib. Tekh. Eksp., 1981, no. 4, p. 89.

  13. Laemmli, U.K., Nature, 1970, vol. 227, no. 259, p. 680.

    Google Scholar 

  14. TSK-Gel Toyopearl, TOYO SODA Manufacturing Co., LTD.

  15. Arakawa, T. and Kita, Y., J. Pharm. Sci., 2000, vol. 89, p. 646.

    Google Scholar 

  16. Sigma-Aldrich 2000-2001, Sigma-Aldrich Co.

  17. Ross, S., LabPlus Int., 2000, vol. 14, no. 4, p. 26.

    Google Scholar 

  18. Squire, P.G., Moser, P.O., and Konski, C.T., Biochemistry, 1968, vol. 7, no. 12, p. 4261.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kapitsa Institute for Physical Problems, Russian Academy of Sciences, ul. Kosygina 2, Moscow, 117334, Russia

    E. L. Kosarev

  2. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia

    K. O. Muranov

Authors
  1. E. L. Kosarev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. O. Muranov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kosarev, E.L., Muranov, K.O. Superresolution Chromatography. Instruments and Experimental Techniques 44, 638–643 (2001). https://doi.org/10.1023/A:1012345507134

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1012345507134

Keywords

Search

Navigation